Cargando…

Treatment of Epidermal Growth Factor (EGF) enhances Nuclear Maturation of Porcine Oocytes and Stimulates Expression of ER/Golgi Transport Proteins

This study was conducted to investigate stimulatory effect of epidermal growth factor (EGF) on nuclear maturation and the expression level of EGF-receptor (EGFR), GM-130 (a marker of Golgi apparatus), transport protein Sec61 subunit beta (Sec61β), and coatomer protein complex subunit gamma 2 (COPG2)...

Descripción completa

Detalles Bibliográficos
Autores principales: Yong, Hwangbo, Oh, Hae-In, Lee, Sang-Hee, Cheong, Hee-Tae, Yang, Boo-Keun, Park, Choon-Keun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Developmental Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5532305/
https://www.ncbi.nlm.nih.gov/pubmed/28785734
http://dx.doi.org/10.12717/DR.2017.21.2.131
_version_ 1783253429838151680
author Yong, Hwangbo
Oh, Hae-In
Lee, Sang-Hee
Cheong, Hee-Tae
Yang, Boo-Keun
Park, Choon-Keun
author_facet Yong, Hwangbo
Oh, Hae-In
Lee, Sang-Hee
Cheong, Hee-Tae
Yang, Boo-Keun
Park, Choon-Keun
author_sort Yong, Hwangbo
collection PubMed
description This study was conducted to investigate stimulatory effect of epidermal growth factor (EGF) on nuclear maturation and the expression level of EGF-receptor (EGFR), GM-130 (a marker of Golgi apparatus), transport protein Sec61 subunit beta (Sec61β), and coatomer protein complex subunit gamma 2 (COPG2) in porcine oocytes. The cumulus-oocyte complexes were collected from follicle with 3-6 mm in diameter. They were incubated in medium with/without EGF for 22 h (IVMⅠ) and subsequently incubated hormone-free medium with/without EGF for 22 h (IVMⅡ). Nuclear maturation state was checked by aceto-orcein stain. Protein expression of EGFR, GM-130, Sec61β, and COPG2 were measured by immunofluorescence. In results, nuclear maturation of oocytes in EGF non-treated oocytes were significantly lower than EGF-treated groups at IVMⅠ or IVMⅡ stage (P<0.05), whereas maturational rate in EGF treatment groups at both of IVM stage was higher in among the all treatment groups (P<0.05). EGFR, GM-130, Sec61β and COPG2 were expressed in the cytoplasm of oocytes. Especially, GM-130 and EGFR were strongly expressed, but Sec61β and COPG2 were weakly expressed in cortical area of cytoplasm. The protein level of GM-130, Sec61β, and COPG2 were significantly higher in the EGF-treated groups (P<0.05). However EGFR was no difference between non EGF-treated groups and control. In conclusion, EGF plays an important role in the systems for oocyte maturation with endoplasmic reticulum and Golgi apparatus. In addition, the protein levels of Sec61β and COPG2 could be changed by EGF in the porcine oocytes during maturation.
format Online
Article
Text
id pubmed-5532305
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher The Korean Society of Developmental Biology
record_format MEDLINE/PubMed
spelling pubmed-55323052017-08-07 Treatment of Epidermal Growth Factor (EGF) enhances Nuclear Maturation of Porcine Oocytes and Stimulates Expression of ER/Golgi Transport Proteins Yong, Hwangbo Oh, Hae-In Lee, Sang-Hee Cheong, Hee-Tae Yang, Boo-Keun Park, Choon-Keun Dev Reprod Original Research Paper This study was conducted to investigate stimulatory effect of epidermal growth factor (EGF) on nuclear maturation and the expression level of EGF-receptor (EGFR), GM-130 (a marker of Golgi apparatus), transport protein Sec61 subunit beta (Sec61β), and coatomer protein complex subunit gamma 2 (COPG2) in porcine oocytes. The cumulus-oocyte complexes were collected from follicle with 3-6 mm in diameter. They were incubated in medium with/without EGF for 22 h (IVMⅠ) and subsequently incubated hormone-free medium with/without EGF for 22 h (IVMⅡ). Nuclear maturation state was checked by aceto-orcein stain. Protein expression of EGFR, GM-130, Sec61β, and COPG2 were measured by immunofluorescence. In results, nuclear maturation of oocytes in EGF non-treated oocytes were significantly lower than EGF-treated groups at IVMⅠ or IVMⅡ stage (P<0.05), whereas maturational rate in EGF treatment groups at both of IVM stage was higher in among the all treatment groups (P<0.05). EGFR, GM-130, Sec61β and COPG2 were expressed in the cytoplasm of oocytes. Especially, GM-130 and EGFR were strongly expressed, but Sec61β and COPG2 were weakly expressed in cortical area of cytoplasm. The protein level of GM-130, Sec61β, and COPG2 were significantly higher in the EGF-treated groups (P<0.05). However EGFR was no difference between non EGF-treated groups and control. In conclusion, EGF plays an important role in the systems for oocyte maturation with endoplasmic reticulum and Golgi apparatus. In addition, the protein levels of Sec61β and COPG2 could be changed by EGF in the porcine oocytes during maturation. The Korean Society of Developmental Biology 2017-06 2017-06-30 /pmc/articles/PMC5532305/ /pubmed/28785734 http://dx.doi.org/10.12717/DR.2017.21.2.131 Text en ⓒ Copyright an Official Journal of the Korean Society of Developmental Biology. All Rights Reserved http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Paper
Yong, Hwangbo
Oh, Hae-In
Lee, Sang-Hee
Cheong, Hee-Tae
Yang, Boo-Keun
Park, Choon-Keun
Treatment of Epidermal Growth Factor (EGF) enhances Nuclear Maturation of Porcine Oocytes and Stimulates Expression of ER/Golgi Transport Proteins
title Treatment of Epidermal Growth Factor (EGF) enhances Nuclear Maturation of Porcine Oocytes and Stimulates Expression of ER/Golgi Transport Proteins
title_full Treatment of Epidermal Growth Factor (EGF) enhances Nuclear Maturation of Porcine Oocytes and Stimulates Expression of ER/Golgi Transport Proteins
title_fullStr Treatment of Epidermal Growth Factor (EGF) enhances Nuclear Maturation of Porcine Oocytes and Stimulates Expression of ER/Golgi Transport Proteins
title_full_unstemmed Treatment of Epidermal Growth Factor (EGF) enhances Nuclear Maturation of Porcine Oocytes and Stimulates Expression of ER/Golgi Transport Proteins
title_short Treatment of Epidermal Growth Factor (EGF) enhances Nuclear Maturation of Porcine Oocytes and Stimulates Expression of ER/Golgi Transport Proteins
title_sort treatment of epidermal growth factor (egf) enhances nuclear maturation of porcine oocytes and stimulates expression of er/golgi transport proteins
topic Original Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5532305/
https://www.ncbi.nlm.nih.gov/pubmed/28785734
http://dx.doi.org/10.12717/DR.2017.21.2.131
work_keys_str_mv AT yonghwangbo treatmentofepidermalgrowthfactoregfenhancesnuclearmaturationofporcineoocytesandstimulatesexpressionofergolgitransportproteins
AT ohhaein treatmentofepidermalgrowthfactoregfenhancesnuclearmaturationofporcineoocytesandstimulatesexpressionofergolgitransportproteins
AT leesanghee treatmentofepidermalgrowthfactoregfenhancesnuclearmaturationofporcineoocytesandstimulatesexpressionofergolgitransportproteins
AT cheongheetae treatmentofepidermalgrowthfactoregfenhancesnuclearmaturationofporcineoocytesandstimulatesexpressionofergolgitransportproteins
AT yangbookeun treatmentofepidermalgrowthfactoregfenhancesnuclearmaturationofporcineoocytesandstimulatesexpressionofergolgitransportproteins
AT parkchoonkeun treatmentofepidermalgrowthfactoregfenhancesnuclearmaturationofporcineoocytesandstimulatesexpressionofergolgitransportproteins