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DNA Microarray Analysis of Estrogen Responsive Genes in Ishikawa Cells by Glabridin

Based on a previous study, glabridin displayed a dose-dependent increase in estrogenic activity and cell proliferative activity in Ishikawa cells. However, when treated in combination with 17β-E(2), synergistic estrogenic effect was observed but without the same synergistic increase in cell prolifer...

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Detalles Bibliográficos
Autores principales: Melissa, Poh Su Wei, Phelim, Yong Voon Chen, Navaratnam, Visweswaran, Yoke Yin, Chia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5533267/
https://www.ncbi.nlm.nih.gov/pubmed/28804245
http://dx.doi.org/10.1177/1178626417721676
Descripción
Sumario:Based on a previous study, glabridin displayed a dose-dependent increase in estrogenic activity and cell proliferative activity in Ishikawa cells. However, when treated in combination with 17β-E(2), synergistic estrogenic effect was observed but without the same synergistic increase in cell proliferative effect. This study aimed to identify the estrogen and nonestrogen-regulated activities induced by glabridin and in combination with 17β-E(2) in comparison with 17β-E(2). The results showed that 10 µM glabridin and the combination treatment of 100 nM glabridin with 1 nM 17β-E(2) regulated both the genomic and nongenomic estrogen pathways to possibly provide benefits of estrogens in cardiovascular, circulatory, and vasculature systems. Meanwhile, the combination of 100 nM glabridin with 1 nM 17β-E(2) seems to be more suitable to be used as an estrogen replacement. Finally, the results of this study have added on to the present knowledge of glabridin’s function as a phytoestrogen and suggested new ideas for the usage of glabridin.