PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping

We developed a novel method, PyroTyping, for discrimination of Mycobacterium tuberculosis isolates combining pyrosequencing and IS6110 polymorphism. A total of 100 isolates were analysed with IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, mycobacterial interspersed repetitive...

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Autores principales: Molina-Moya, B., Lacoma, A., García-Sierra, N., Blanco, S., Haba, L., Samper, S., Ruiz-Manzano, J., Prat, C., Arnold, C., Domínguez, J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5533701/
https://www.ncbi.nlm.nih.gov/pubmed/28754991
http://dx.doi.org/10.1038/s41598-017-06760-5
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author Molina-Moya, B.
Lacoma, A.
García-Sierra, N.
Blanco, S.
Haba, L.
Samper, S.
Ruiz-Manzano, J.
Prat, C.
Arnold, C.
Domínguez, J.
author_facet Molina-Moya, B.
Lacoma, A.
García-Sierra, N.
Blanco, S.
Haba, L.
Samper, S.
Ruiz-Manzano, J.
Prat, C.
Arnold, C.
Domínguez, J.
author_sort Molina-Moya, B.
collection PubMed
description We developed a novel method, PyroTyping, for discrimination of Mycobacterium tuberculosis isolates combining pyrosequencing and IS6110 polymorphism. A total of 100 isolates were analysed with IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, mycobacterial interspersed repetitive units – variable number tandem repeats (MIRU-VNTR), and PyroTyping. PyroTyping results regarding clustering or discrimination of the isolates were highly concordant with the other typing methods performed. PyroTyping is more rapid than RFLP and presents the same discriminatory power, thus, it may be useful for taking timely decisions for tuberculosis control.
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spelling pubmed-55337012017-08-03 PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping Molina-Moya, B. Lacoma, A. García-Sierra, N. Blanco, S. Haba, L. Samper, S. Ruiz-Manzano, J. Prat, C. Arnold, C. Domínguez, J. Sci Rep Article We developed a novel method, PyroTyping, for discrimination of Mycobacterium tuberculosis isolates combining pyrosequencing and IS6110 polymorphism. A total of 100 isolates were analysed with IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, mycobacterial interspersed repetitive units – variable number tandem repeats (MIRU-VNTR), and PyroTyping. PyroTyping results regarding clustering or discrimination of the isolates were highly concordant with the other typing methods performed. PyroTyping is more rapid than RFLP and presents the same discriminatory power, thus, it may be useful for taking timely decisions for tuberculosis control. Nature Publishing Group UK 2017-07-28 /pmc/articles/PMC5533701/ /pubmed/28754991 http://dx.doi.org/10.1038/s41598-017-06760-5 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Molina-Moya, B.
Lacoma, A.
García-Sierra, N.
Blanco, S.
Haba, L.
Samper, S.
Ruiz-Manzano, J.
Prat, C.
Arnold, C.
Domínguez, J.
PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping
title PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping
title_full PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping
title_fullStr PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping
title_full_unstemmed PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping
title_short PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping
title_sort pyrotyping, a novel pyrosequencing-based assay for mycobacterium tuberculosis genotyping
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5533701/
https://www.ncbi.nlm.nih.gov/pubmed/28754991
http://dx.doi.org/10.1038/s41598-017-06760-5
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