Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy

The polarity of microtubules is thought to be involved in spindle assembly, cytokinesis or active molecular transport. However, its exact role remains poorly understood, mainly because of the challenge to measure microtubule polarity in intact cells. We report here the use of fast Interferometric Se...

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Autores principales: Bancelin, S., Couture, C.-A., Pinsard, M., Rivard, M., Drapeau, P., Légaré, F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5533768/
https://www.ncbi.nlm.nih.gov/pubmed/28754928
http://dx.doi.org/10.1038/s41598-017-06648-4
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author Bancelin, S.
Couture, C.-A.
Pinsard, M.
Rivard, M.
Drapeau, P.
Légaré, F.
author_facet Bancelin, S.
Couture, C.-A.
Pinsard, M.
Rivard, M.
Drapeau, P.
Légaré, F.
author_sort Bancelin, S.
collection PubMed
description The polarity of microtubules is thought to be involved in spindle assembly, cytokinesis or active molecular transport. However, its exact role remains poorly understood, mainly because of the challenge to measure microtubule polarity in intact cells. We report here the use of fast Interferometric Second Harmonic Generation microscopy to study the polarity of microtubules forming the mitotic spindles in a zebrafish embryo. This technique provides a powerful tool to study mitotic spindle formation and may be directly transferable for investigating the kinetics and function of microtubule polarity in other aspects of subcellular motility or in native tissues.
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spelling pubmed-55337682017-08-03 Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy Bancelin, S. Couture, C.-A. Pinsard, M. Rivard, M. Drapeau, P. Légaré, F. Sci Rep Article The polarity of microtubules is thought to be involved in spindle assembly, cytokinesis or active molecular transport. However, its exact role remains poorly understood, mainly because of the challenge to measure microtubule polarity in intact cells. We report here the use of fast Interferometric Second Harmonic Generation microscopy to study the polarity of microtubules forming the mitotic spindles in a zebrafish embryo. This technique provides a powerful tool to study mitotic spindle formation and may be directly transferable for investigating the kinetics and function of microtubule polarity in other aspects of subcellular motility or in native tissues. Nature Publishing Group UK 2017-07-28 /pmc/articles/PMC5533768/ /pubmed/28754928 http://dx.doi.org/10.1038/s41598-017-06648-4 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bancelin, S.
Couture, C.-A.
Pinsard, M.
Rivard, M.
Drapeau, P.
Légaré, F.
Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy
title Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy
title_full Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy
title_fullStr Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy
title_full_unstemmed Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy
title_short Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy
title_sort probing microtubules polarity in mitotic spindles in situ using interferometric second harmonic generation microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5533768/
https://www.ncbi.nlm.nih.gov/pubmed/28754928
http://dx.doi.org/10.1038/s41598-017-06648-4
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