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High-throughput mutagenesis using a two-fragment PCR approach

Site-directed scanning mutagenesis is a powerful protein engineering technique which allows studies of protein functionality at single amino acid resolution and design of stabilized proteins for structural and biophysical work. However, creating libraries of hundreds of mutants remains a challenging...

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Detalles Bibliográficos
Autores principales:	Heydenreich, Franziska M., Miljuš, Tamara, Jaussi, Rolf, Benoit, Roger, Milić, Dalibor, Veprintsev, Dmitry B.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5533798/ https://www.ncbi.nlm.nih.gov/pubmed/28754896 http://dx.doi.org/10.1038/s41598-017-07010-4

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