DNA Origami offers a versatile method for quantifying protein copy-number in super-resolution

Single-molecule-based super-resolution microscopy offers a unique opportunity for quantifying protein copy-number with nanoscale resolution. However, while fluorescent proteins have been characterized for quantitative imaging using calibration standards, similar calibration tools for immunofluoresce...

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Detalles Bibliográficos
Autores principales: Zanacchi, Francesca Cella, Manzo, Carlo, Alvarez, Angel Sandoval, Derr, Nathan D., Parajo, Maria Garcia, Lakadamyali, Melike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5534338/
https://www.ncbi.nlm.nih.gov/pubmed/28650478
http://dx.doi.org/10.1038/nmeth.4342
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author Zanacchi, Francesca Cella
Manzo, Carlo
Alvarez, Angel Sandoval
Derr, Nathan D.
Parajo, Maria Garcia
Lakadamyali, Melike
author_facet Zanacchi, Francesca Cella
Manzo, Carlo
Alvarez, Angel Sandoval
Derr, Nathan D.
Parajo, Maria Garcia
Lakadamyali, Melike
author_sort Zanacchi, Francesca Cella
collection PubMed
description Single-molecule-based super-resolution microscopy offers a unique opportunity for quantifying protein copy-number with nanoscale resolution. However, while fluorescent proteins have been characterized for quantitative imaging using calibration standards, similar calibration tools for immunofluorescence with small organic fluorophores are lacking. Here, we show that DNA origami in combination with GFP antibodies is a versatile platform for calibrating fluorophore and antibody labeling efficiency to quantify protein copy-number in cellular contexts using super-resolution microscopy.
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spelling pubmed-55343382017-12-26 DNA Origami offers a versatile method for quantifying protein copy-number in super-resolution Zanacchi, Francesca Cella Manzo, Carlo Alvarez, Angel Sandoval Derr, Nathan D. Parajo, Maria Garcia Lakadamyali, Melike Nat Methods Article Single-molecule-based super-resolution microscopy offers a unique opportunity for quantifying protein copy-number with nanoscale resolution. However, while fluorescent proteins have been characterized for quantitative imaging using calibration standards, similar calibration tools for immunofluorescence with small organic fluorophores are lacking. Here, we show that DNA origami in combination with GFP antibodies is a versatile platform for calibrating fluorophore and antibody labeling efficiency to quantify protein copy-number in cellular contexts using super-resolution microscopy. 2017-06-26 2017-08 /pmc/articles/PMC5534338/ /pubmed/28650478 http://dx.doi.org/10.1038/nmeth.4342 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Zanacchi, Francesca Cella
Manzo, Carlo
Alvarez, Angel Sandoval
Derr, Nathan D.
Parajo, Maria Garcia
Lakadamyali, Melike
DNA Origami offers a versatile method for quantifying protein copy-number in super-resolution
title DNA Origami offers a versatile method for quantifying protein copy-number in super-resolution
title_full DNA Origami offers a versatile method for quantifying protein copy-number in super-resolution
title_fullStr DNA Origami offers a versatile method for quantifying protein copy-number in super-resolution
title_full_unstemmed DNA Origami offers a versatile method for quantifying protein copy-number in super-resolution
title_short DNA Origami offers a versatile method for quantifying protein copy-number in super-resolution
title_sort dna origami offers a versatile method for quantifying protein copy-number in super-resolution
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5534338/
https://www.ncbi.nlm.nih.gov/pubmed/28650478
http://dx.doi.org/10.1038/nmeth.4342
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