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The Influence of Copolymer Composition on PLGA/nHA Scaffolds’ Cytotoxicity and In Vitro Degradation

The influence of copolymer composition on Poly(Lactide-co-Glycolide)/nanohydroxyapatite (PLGA/nHA) composite scaffolds is studied in the context of bone tissue engineering and regenerative medicine. The composite scaffolds are fabricated by thermally-induced phase separation and the effect of bioact...

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Detalles Bibliográficos
Autores principales: Díaz, Esperanza, Puerto, Igor, Ribeiro, Silvie, Lanceros-Mendez, Senentxu, Barandiarán, José Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535239/
https://www.ncbi.nlm.nih.gov/pubmed/28684725
http://dx.doi.org/10.3390/nano7070173
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author Díaz, Esperanza
Puerto, Igor
Ribeiro, Silvie
Lanceros-Mendez, Senentxu
Barandiarán, José Manuel
author_facet Díaz, Esperanza
Puerto, Igor
Ribeiro, Silvie
Lanceros-Mendez, Senentxu
Barandiarán, José Manuel
author_sort Díaz, Esperanza
collection PubMed
description The influence of copolymer composition on Poly(Lactide-co-Glycolide)/nanohydroxyapatite (PLGA/nHA) composite scaffolds is studied in the context of bone tissue engineering and regenerative medicine. The composite scaffolds are fabricated by thermally-induced phase separation and the effect of bioactive nanoparticles on their in vitro degradation in phosphate-buffered solution at 37 °C is analyzed over eight weeks. The indirect cytotoxicity evaluation of the samples followed an adaptation of the ISO 10993-5 standard test method. Based on the measurement of their molecular weight, molar mass, pH, water absorption and dimensions, the porous scaffolds of PLGA with a lower lactide/glycolide (LA/GA) molar ratio degraded faster due to their higher hydrophilicity. All of the samples without and with HA are not cytotoxic, demonstrating their potential for tissue engineering applications.
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spelling pubmed-55352392017-08-04 The Influence of Copolymer Composition on PLGA/nHA Scaffolds’ Cytotoxicity and In Vitro Degradation Díaz, Esperanza Puerto, Igor Ribeiro, Silvie Lanceros-Mendez, Senentxu Barandiarán, José Manuel Nanomaterials (Basel) Article The influence of copolymer composition on Poly(Lactide-co-Glycolide)/nanohydroxyapatite (PLGA/nHA) composite scaffolds is studied in the context of bone tissue engineering and regenerative medicine. The composite scaffolds are fabricated by thermally-induced phase separation and the effect of bioactive nanoparticles on their in vitro degradation in phosphate-buffered solution at 37 °C is analyzed over eight weeks. The indirect cytotoxicity evaluation of the samples followed an adaptation of the ISO 10993-5 standard test method. Based on the measurement of their molecular weight, molar mass, pH, water absorption and dimensions, the porous scaffolds of PLGA with a lower lactide/glycolide (LA/GA) molar ratio degraded faster due to their higher hydrophilicity. All of the samples without and with HA are not cytotoxic, demonstrating their potential for tissue engineering applications. MDPI 2017-07-06 /pmc/articles/PMC5535239/ /pubmed/28684725 http://dx.doi.org/10.3390/nano7070173 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Díaz, Esperanza
Puerto, Igor
Ribeiro, Silvie
Lanceros-Mendez, Senentxu
Barandiarán, José Manuel
The Influence of Copolymer Composition on PLGA/nHA Scaffolds’ Cytotoxicity and In Vitro Degradation
title The Influence of Copolymer Composition on PLGA/nHA Scaffolds’ Cytotoxicity and In Vitro Degradation
title_full The Influence of Copolymer Composition on PLGA/nHA Scaffolds’ Cytotoxicity and In Vitro Degradation
title_fullStr The Influence of Copolymer Composition on PLGA/nHA Scaffolds’ Cytotoxicity and In Vitro Degradation
title_full_unstemmed The Influence of Copolymer Composition on PLGA/nHA Scaffolds’ Cytotoxicity and In Vitro Degradation
title_short The Influence of Copolymer Composition on PLGA/nHA Scaffolds’ Cytotoxicity and In Vitro Degradation
title_sort influence of copolymer composition on plga/nha scaffolds’ cytotoxicity and in vitro degradation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535239/
https://www.ncbi.nlm.nih.gov/pubmed/28684725
http://dx.doi.org/10.3390/nano7070173
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