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The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy

Silver nanoparticles (AgNPs) have been demonstrated to restrain bacterial growth, while maintaining minimal risk in development of bacterial resistance and human cell toxicity that conventional silver compounds exhibit. Several physical and chemical methods have been reported to synthesize AgNPs. Ho...

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Autores principales: Skandalis, Nicholas, Dimopoulou, Anastasia, Georgopoulou, Anthie, Gallios, Nikolaos, Papadopoulos, Dimitrios, Tsipas, Dimitrios, Theologidis, Ioannis, Michailidis, Nikolaos, Chatzinikolaidou, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535244/
https://www.ncbi.nlm.nih.gov/pubmed/28698511
http://dx.doi.org/10.3390/nano7070178
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author Skandalis, Nicholas
Dimopoulou, Anastasia
Georgopoulou, Anthie
Gallios, Nikolaos
Papadopoulos, Dimitrios
Tsipas, Dimitrios
Theologidis, Ioannis
Michailidis, Nikolaos
Chatzinikolaidou, Maria
author_facet Skandalis, Nicholas
Dimopoulou, Anastasia
Georgopoulou, Anthie
Gallios, Nikolaos
Papadopoulos, Dimitrios
Tsipas, Dimitrios
Theologidis, Ioannis
Michailidis, Nikolaos
Chatzinikolaidou, Maria
author_sort Skandalis, Nicholas
collection PubMed
description Silver nanoparticles (AgNPs) have been demonstrated to restrain bacterial growth, while maintaining minimal risk in development of bacterial resistance and human cell toxicity that conventional silver compounds exhibit. Several physical and chemical methods have been reported to synthesize AgNPs. However, these methods are expensive and involve heavy chemical reduction agents. An alternative approach to produce AgNPs in a cost-effective and environmentally friendly way employs a biological pathway using various plant extracts to reduce metal ions. The size control issue, and the stability of nanoparticles, remain some of the latest challenges in such methods. In this study, we used two different concentrations of fresh leaf extract of the plant Arbutus unedo (LEA) as a reducing and stabilizing agent to produce two size variations of AgNPs. UV-Vis spectroscopy, Dynamic Light Scattering, Transmission Electron Microscopy, and zeta potential were applied for the characterization of AgNPs. Both AgNP variations were evaluated for their antibacterial efficacy against the gram-negative species Escherichia coli and Pseudomonas aeruginosa, as well as the gram-positive species Bacillus subtilis and Staphylococcus epidermidis. Although significant differences have been achieved in the nanoparticles’ size by varying the plant extract concentration during synthesis, the antibacterial effect was almost the same.
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spelling pubmed-55352442017-08-04 The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy Skandalis, Nicholas Dimopoulou, Anastasia Georgopoulou, Anthie Gallios, Nikolaos Papadopoulos, Dimitrios Tsipas, Dimitrios Theologidis, Ioannis Michailidis, Nikolaos Chatzinikolaidou, Maria Nanomaterials (Basel) Article Silver nanoparticles (AgNPs) have been demonstrated to restrain bacterial growth, while maintaining minimal risk in development of bacterial resistance and human cell toxicity that conventional silver compounds exhibit. Several physical and chemical methods have been reported to synthesize AgNPs. However, these methods are expensive and involve heavy chemical reduction agents. An alternative approach to produce AgNPs in a cost-effective and environmentally friendly way employs a biological pathway using various plant extracts to reduce metal ions. The size control issue, and the stability of nanoparticles, remain some of the latest challenges in such methods. In this study, we used two different concentrations of fresh leaf extract of the plant Arbutus unedo (LEA) as a reducing and stabilizing agent to produce two size variations of AgNPs. UV-Vis spectroscopy, Dynamic Light Scattering, Transmission Electron Microscopy, and zeta potential were applied for the characterization of AgNPs. Both AgNP variations were evaluated for their antibacterial efficacy against the gram-negative species Escherichia coli and Pseudomonas aeruginosa, as well as the gram-positive species Bacillus subtilis and Staphylococcus epidermidis. Although significant differences have been achieved in the nanoparticles’ size by varying the plant extract concentration during synthesis, the antibacterial effect was almost the same. MDPI 2017-07-10 /pmc/articles/PMC5535244/ /pubmed/28698511 http://dx.doi.org/10.3390/nano7070178 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Skandalis, Nicholas
Dimopoulou, Anastasia
Georgopoulou, Anthie
Gallios, Nikolaos
Papadopoulos, Dimitrios
Tsipas, Dimitrios
Theologidis, Ioannis
Michailidis, Nikolaos
Chatzinikolaidou, Maria
The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy
title The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy
title_full The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy
title_fullStr The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy
title_full_unstemmed The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy
title_short The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy
title_sort effect of silver nanoparticles size, produced using plant extract from arbutus unedo, on their antibacterial efficacy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535244/
https://www.ncbi.nlm.nih.gov/pubmed/28698511
http://dx.doi.org/10.3390/nano7070178
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