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The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy
Silver nanoparticles (AgNPs) have been demonstrated to restrain bacterial growth, while maintaining minimal risk in development of bacterial resistance and human cell toxicity that conventional silver compounds exhibit. Several physical and chemical methods have been reported to synthesize AgNPs. Ho...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535244/ https://www.ncbi.nlm.nih.gov/pubmed/28698511 http://dx.doi.org/10.3390/nano7070178 |
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author | Skandalis, Nicholas Dimopoulou, Anastasia Georgopoulou, Anthie Gallios, Nikolaos Papadopoulos, Dimitrios Tsipas, Dimitrios Theologidis, Ioannis Michailidis, Nikolaos Chatzinikolaidou, Maria |
author_facet | Skandalis, Nicholas Dimopoulou, Anastasia Georgopoulou, Anthie Gallios, Nikolaos Papadopoulos, Dimitrios Tsipas, Dimitrios Theologidis, Ioannis Michailidis, Nikolaos Chatzinikolaidou, Maria |
author_sort | Skandalis, Nicholas |
collection | PubMed |
description | Silver nanoparticles (AgNPs) have been demonstrated to restrain bacterial growth, while maintaining minimal risk in development of bacterial resistance and human cell toxicity that conventional silver compounds exhibit. Several physical and chemical methods have been reported to synthesize AgNPs. However, these methods are expensive and involve heavy chemical reduction agents. An alternative approach to produce AgNPs in a cost-effective and environmentally friendly way employs a biological pathway using various plant extracts to reduce metal ions. The size control issue, and the stability of nanoparticles, remain some of the latest challenges in such methods. In this study, we used two different concentrations of fresh leaf extract of the plant Arbutus unedo (LEA) as a reducing and stabilizing agent to produce two size variations of AgNPs. UV-Vis spectroscopy, Dynamic Light Scattering, Transmission Electron Microscopy, and zeta potential were applied for the characterization of AgNPs. Both AgNP variations were evaluated for their antibacterial efficacy against the gram-negative species Escherichia coli and Pseudomonas aeruginosa, as well as the gram-positive species Bacillus subtilis and Staphylococcus epidermidis. Although significant differences have been achieved in the nanoparticles’ size by varying the plant extract concentration during synthesis, the antibacterial effect was almost the same. |
format | Online Article Text |
id | pubmed-5535244 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-55352442017-08-04 The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy Skandalis, Nicholas Dimopoulou, Anastasia Georgopoulou, Anthie Gallios, Nikolaos Papadopoulos, Dimitrios Tsipas, Dimitrios Theologidis, Ioannis Michailidis, Nikolaos Chatzinikolaidou, Maria Nanomaterials (Basel) Article Silver nanoparticles (AgNPs) have been demonstrated to restrain bacterial growth, while maintaining minimal risk in development of bacterial resistance and human cell toxicity that conventional silver compounds exhibit. Several physical and chemical methods have been reported to synthesize AgNPs. However, these methods are expensive and involve heavy chemical reduction agents. An alternative approach to produce AgNPs in a cost-effective and environmentally friendly way employs a biological pathway using various plant extracts to reduce metal ions. The size control issue, and the stability of nanoparticles, remain some of the latest challenges in such methods. In this study, we used two different concentrations of fresh leaf extract of the plant Arbutus unedo (LEA) as a reducing and stabilizing agent to produce two size variations of AgNPs. UV-Vis spectroscopy, Dynamic Light Scattering, Transmission Electron Microscopy, and zeta potential were applied for the characterization of AgNPs. Both AgNP variations were evaluated for their antibacterial efficacy against the gram-negative species Escherichia coli and Pseudomonas aeruginosa, as well as the gram-positive species Bacillus subtilis and Staphylococcus epidermidis. Although significant differences have been achieved in the nanoparticles’ size by varying the plant extract concentration during synthesis, the antibacterial effect was almost the same. MDPI 2017-07-10 /pmc/articles/PMC5535244/ /pubmed/28698511 http://dx.doi.org/10.3390/nano7070178 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Skandalis, Nicholas Dimopoulou, Anastasia Georgopoulou, Anthie Gallios, Nikolaos Papadopoulos, Dimitrios Tsipas, Dimitrios Theologidis, Ioannis Michailidis, Nikolaos Chatzinikolaidou, Maria The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy |
title | The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy |
title_full | The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy |
title_fullStr | The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy |
title_full_unstemmed | The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy |
title_short | The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy |
title_sort | effect of silver nanoparticles size, produced using plant extract from arbutus unedo, on their antibacterial efficacy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535244/ https://www.ncbi.nlm.nih.gov/pubmed/28698511 http://dx.doi.org/10.3390/nano7070178 |
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