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Usefulness of Intravital Multiphoton Microscopy in Visualizing Study of Mouse Cochlea and Volume Changes in the Scala Media

Conventional microscopy has limitations in viewing the cochlear microstructures due to three-dimensional spiral structure and the overlying bone. But these issues can be overcome by imaging the cochlea in vitro with intravital multiphoton microscopy (MPM). By using near-infrared lasers for multiphot...

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Autores principales: Ju, Hyun Mi, Lee, Sun Hee, Kong, Tae Hoon, Kwon, Seung-Hae, Choi, Jin Sil, Seo, Young Joon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535263/
https://www.ncbi.nlm.nih.gov/pubmed/28824523
http://dx.doi.org/10.3389/fneur.2017.00332
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author Ju, Hyun Mi
Lee, Sun Hee
Kong, Tae Hoon
Kwon, Seung-Hae
Choi, Jin Sil
Seo, Young Joon
author_facet Ju, Hyun Mi
Lee, Sun Hee
Kong, Tae Hoon
Kwon, Seung-Hae
Choi, Jin Sil
Seo, Young Joon
author_sort Ju, Hyun Mi
collection PubMed
description Conventional microscopy has limitations in viewing the cochlear microstructures due to three-dimensional spiral structure and the overlying bone. But these issues can be overcome by imaging the cochlea in vitro with intravital multiphoton microscopy (MPM). By using near-infrared lasers for multiphoton excitation, intravital MPM can detect endogenous fluorescence and second harmonic generation of tissues. In this study, we used intravital MPM to visualize various cochlear microstructures without any staining and non-invasively analyze the volume changes of the scala media (SM) without removing the overlying cochlear bone. The intravital MPM images revealed various tissue types, ranging from thin membranes to dense bone, as well as the spiral ganglion beneath the cochlear bone. The two-dimensional, cross-sectional, and serial z-stack intravital MPM images also revealed the spatial dilation of the SM in the temporal bone of pendrin-deficient mice. These findings suggest that intravital MPM might serve as a new method for obtaining microanatomical information regarding the cochlea, similar to standard histopathological analyses in the animal study for the cochlea. Given the capability of intravital MPM for detecting an increase in the volume of the SM in pendrin-deficient mice, it might be a promising new tool for assessing the pathophysiology of hearing loss in the future.
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spelling pubmed-55352632017-08-18 Usefulness of Intravital Multiphoton Microscopy in Visualizing Study of Mouse Cochlea and Volume Changes in the Scala Media Ju, Hyun Mi Lee, Sun Hee Kong, Tae Hoon Kwon, Seung-Hae Choi, Jin Sil Seo, Young Joon Front Neurol Neuroscience Conventional microscopy has limitations in viewing the cochlear microstructures due to three-dimensional spiral structure and the overlying bone. But these issues can be overcome by imaging the cochlea in vitro with intravital multiphoton microscopy (MPM). By using near-infrared lasers for multiphoton excitation, intravital MPM can detect endogenous fluorescence and second harmonic generation of tissues. In this study, we used intravital MPM to visualize various cochlear microstructures without any staining and non-invasively analyze the volume changes of the scala media (SM) without removing the overlying cochlear bone. The intravital MPM images revealed various tissue types, ranging from thin membranes to dense bone, as well as the spiral ganglion beneath the cochlear bone. The two-dimensional, cross-sectional, and serial z-stack intravital MPM images also revealed the spatial dilation of the SM in the temporal bone of pendrin-deficient mice. These findings suggest that intravital MPM might serve as a new method for obtaining microanatomical information regarding the cochlea, similar to standard histopathological analyses in the animal study for the cochlea. Given the capability of intravital MPM for detecting an increase in the volume of the SM in pendrin-deficient mice, it might be a promising new tool for assessing the pathophysiology of hearing loss in the future. Frontiers Media S.A. 2017-07-31 /pmc/articles/PMC5535263/ /pubmed/28824523 http://dx.doi.org/10.3389/fneur.2017.00332 Text en Copyright © 2017 Ju, Lee, Kong, Kwon, Choi and Seo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Ju, Hyun Mi
Lee, Sun Hee
Kong, Tae Hoon
Kwon, Seung-Hae
Choi, Jin Sil
Seo, Young Joon
Usefulness of Intravital Multiphoton Microscopy in Visualizing Study of Mouse Cochlea and Volume Changes in the Scala Media
title Usefulness of Intravital Multiphoton Microscopy in Visualizing Study of Mouse Cochlea and Volume Changes in the Scala Media
title_full Usefulness of Intravital Multiphoton Microscopy in Visualizing Study of Mouse Cochlea and Volume Changes in the Scala Media
title_fullStr Usefulness of Intravital Multiphoton Microscopy in Visualizing Study of Mouse Cochlea and Volume Changes in the Scala Media
title_full_unstemmed Usefulness of Intravital Multiphoton Microscopy in Visualizing Study of Mouse Cochlea and Volume Changes in the Scala Media
title_short Usefulness of Intravital Multiphoton Microscopy in Visualizing Study of Mouse Cochlea and Volume Changes in the Scala Media
title_sort usefulness of intravital multiphoton microscopy in visualizing study of mouse cochlea and volume changes in the scala media
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535263/
https://www.ncbi.nlm.nih.gov/pubmed/28824523
http://dx.doi.org/10.3389/fneur.2017.00332
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