Cargando…

The Ethanolic Stem-Bark Extract of Antrocaryon micraster Inhibits Carrageenan-Induced Pleurisy and Pedal Oedema in Murine Models of Inflammation

We investigated the antioxidant and anti-inflammatory effects of a 70% v/v ethanol extract of the stem bark of Antrocaryon micraster on murine models of carrageenan-induced pleurisy and paw oedema. Rat pleural fluid was analysed for volume, protein content, and leucocytes, while lung histology was a...

Descripción completa

Detalles Bibliográficos
Autores principales: Essel, Leslie B., Obiri, David D., Osafo, Newman, Antwi, Aaron O., Duduyemi, Babatunde M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535746/
https://www.ncbi.nlm.nih.gov/pubmed/28798953
http://dx.doi.org/10.1155/2017/6859230
_version_ 1783253900024872960
author Essel, Leslie B.
Obiri, David D.
Osafo, Newman
Antwi, Aaron O.
Duduyemi, Babatunde M.
author_facet Essel, Leslie B.
Obiri, David D.
Osafo, Newman
Antwi, Aaron O.
Duduyemi, Babatunde M.
author_sort Essel, Leslie B.
collection PubMed
description We investigated the antioxidant and anti-inflammatory effects of a 70% v/v ethanol extract of the stem bark of Antrocaryon micraster on murine models of carrageenan-induced pleurisy and paw oedema. Rat pleural fluid was analysed for volume, protein content, and leucocytes, while lung histology was assessed for damage. Lung tissue homogenates were assayed for glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and myeloperoxidase (MPO). Phytochemical analysis was carried out on the stem bark. Acute toxicity studies were conducted in rats. In the pleurisy model the extract (30–300 mg/kg) significantly reduced the volume and amount of proteins and leucocytes in the exudate and also protected against lung injury. Tissue level of GSH and SOD and CAT expression were increased while MDA level and MPO activity were reduced. The peak and total oedema responses were significantly suppressed when given both preemptively and curatively in the mice paw oedema test. Saponins, alkaloids, triterpenoids, and tannins were present in the stem bark. A. micraster extract exhibited no apparent acute toxicity. We conclude that the ethanolic stem-bark extract of A. micraster has antioxidant action and exhibits significant anti-inflammatory activity through suppression of pleurisy and paw oedema induced with carrageenan.
format Online
Article
Text
id pubmed-5535746
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-55357462017-08-10 The Ethanolic Stem-Bark Extract of Antrocaryon micraster Inhibits Carrageenan-Induced Pleurisy and Pedal Oedema in Murine Models of Inflammation Essel, Leslie B. Obiri, David D. Osafo, Newman Antwi, Aaron O. Duduyemi, Babatunde M. Int Sch Res Notices Research Article We investigated the antioxidant and anti-inflammatory effects of a 70% v/v ethanol extract of the stem bark of Antrocaryon micraster on murine models of carrageenan-induced pleurisy and paw oedema. Rat pleural fluid was analysed for volume, protein content, and leucocytes, while lung histology was assessed for damage. Lung tissue homogenates were assayed for glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and myeloperoxidase (MPO). Phytochemical analysis was carried out on the stem bark. Acute toxicity studies were conducted in rats. In the pleurisy model the extract (30–300 mg/kg) significantly reduced the volume and amount of proteins and leucocytes in the exudate and also protected against lung injury. Tissue level of GSH and SOD and CAT expression were increased while MDA level and MPO activity were reduced. The peak and total oedema responses were significantly suppressed when given both preemptively and curatively in the mice paw oedema test. Saponins, alkaloids, triterpenoids, and tannins were present in the stem bark. A. micraster extract exhibited no apparent acute toxicity. We conclude that the ethanolic stem-bark extract of A. micraster has antioxidant action and exhibits significant anti-inflammatory activity through suppression of pleurisy and paw oedema induced with carrageenan. Hindawi 2017-07-17 /pmc/articles/PMC5535746/ /pubmed/28798953 http://dx.doi.org/10.1155/2017/6859230 Text en Copyright © 2017 Leslie B. Essel et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Essel, Leslie B.
Obiri, David D.
Osafo, Newman
Antwi, Aaron O.
Duduyemi, Babatunde M.
The Ethanolic Stem-Bark Extract of Antrocaryon micraster Inhibits Carrageenan-Induced Pleurisy and Pedal Oedema in Murine Models of Inflammation
title The Ethanolic Stem-Bark Extract of Antrocaryon micraster Inhibits Carrageenan-Induced Pleurisy and Pedal Oedema in Murine Models of Inflammation
title_full The Ethanolic Stem-Bark Extract of Antrocaryon micraster Inhibits Carrageenan-Induced Pleurisy and Pedal Oedema in Murine Models of Inflammation
title_fullStr The Ethanolic Stem-Bark Extract of Antrocaryon micraster Inhibits Carrageenan-Induced Pleurisy and Pedal Oedema in Murine Models of Inflammation
title_full_unstemmed The Ethanolic Stem-Bark Extract of Antrocaryon micraster Inhibits Carrageenan-Induced Pleurisy and Pedal Oedema in Murine Models of Inflammation
title_short The Ethanolic Stem-Bark Extract of Antrocaryon micraster Inhibits Carrageenan-Induced Pleurisy and Pedal Oedema in Murine Models of Inflammation
title_sort ethanolic stem-bark extract of antrocaryon micraster inhibits carrageenan-induced pleurisy and pedal oedema in murine models of inflammation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535746/
https://www.ncbi.nlm.nih.gov/pubmed/28798953
http://dx.doi.org/10.1155/2017/6859230
work_keys_str_mv AT esselleslieb theethanolicstembarkextractofantrocaryonmicrasterinhibitscarrageenaninducedpleurisyandpedaloedemainmurinemodelsofinflammation
AT obiridavidd theethanolicstembarkextractofantrocaryonmicrasterinhibitscarrageenaninducedpleurisyandpedaloedemainmurinemodelsofinflammation
AT osafonewman theethanolicstembarkextractofantrocaryonmicrasterinhibitscarrageenaninducedpleurisyandpedaloedemainmurinemodelsofinflammation
AT antwiaarono theethanolicstembarkextractofantrocaryonmicrasterinhibitscarrageenaninducedpleurisyandpedaloedemainmurinemodelsofinflammation
AT duduyemibabatundem theethanolicstembarkextractofantrocaryonmicrasterinhibitscarrageenaninducedpleurisyandpedaloedemainmurinemodelsofinflammation
AT esselleslieb ethanolicstembarkextractofantrocaryonmicrasterinhibitscarrageenaninducedpleurisyandpedaloedemainmurinemodelsofinflammation
AT obiridavidd ethanolicstembarkextractofantrocaryonmicrasterinhibitscarrageenaninducedpleurisyandpedaloedemainmurinemodelsofinflammation
AT osafonewman ethanolicstembarkextractofantrocaryonmicrasterinhibitscarrageenaninducedpleurisyandpedaloedemainmurinemodelsofinflammation
AT antwiaarono ethanolicstembarkextractofantrocaryonmicrasterinhibitscarrageenaninducedpleurisyandpedaloedemainmurinemodelsofinflammation
AT duduyemibabatundem ethanolicstembarkextractofantrocaryonmicrasterinhibitscarrageenaninducedpleurisyandpedaloedemainmurinemodelsofinflammation