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Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita

Meloidogyne incognita is a common root-knot nematode with a wide range of plant hosts. We aimed to study the metabolites produced at each stage of the nematode life cycle to understand its development. Metabolites of Meloidogyne incognita were extracted at egg, J2, J3, J4, and female stages and 110...

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Autores principales: Subramanian, Parthiban, Oh, Byung-Ju, Mani, Vimalraj, Lee, Jae Kook, Lee, Chang-Muk, Sim, Joon-Soo, Koo, Ja Choon, Hahn, Bum-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535844/
https://www.ncbi.nlm.nih.gov/pubmed/28672815
http://dx.doi.org/10.3390/ijms18071351
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author Subramanian, Parthiban
Oh, Byung-Ju
Mani, Vimalraj
Lee, Jae Kook
Lee, Chang-Muk
Sim, Joon-Soo
Koo, Ja Choon
Hahn, Bum-Soo
author_facet Subramanian, Parthiban
Oh, Byung-Ju
Mani, Vimalraj
Lee, Jae Kook
Lee, Chang-Muk
Sim, Joon-Soo
Koo, Ja Choon
Hahn, Bum-Soo
author_sort Subramanian, Parthiban
collection PubMed
description Meloidogyne incognita is a common root-knot nematode with a wide range of plant hosts. We aimed to study the metabolites produced at each stage of the nematode life cycle to understand its development. Metabolites of Meloidogyne incognita were extracted at egg, J2, J3, J4, and female stages and 110 metabolites with available standards were quantified using CE-TOF/MS. Analyses indicated abundance of stage-specific metabolites with the exception of J3 and J4 stages which shared similar metabolic profiles. The egg stage showed increased abundance in glycolysis and energy metabolism related metabolites while the J2 metabolites are associated with tissue formation, motility, and neurotransmission. The J3 and J4 stages indicated amino acid metabolism and urea cycle- related metabolites. The female stage was characterized with polyamine synthesis, antioxidant activity, and synthesis of reproduction related metabolites. Such metabolic profiling helps us understand the dynamic physiological changes related to each developmental stage of the root-knot nematode life cycle.
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spelling pubmed-55358442017-08-04 Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita Subramanian, Parthiban Oh, Byung-Ju Mani, Vimalraj Lee, Jae Kook Lee, Chang-Muk Sim, Joon-Soo Koo, Ja Choon Hahn, Bum-Soo Int J Mol Sci Communication Meloidogyne incognita is a common root-knot nematode with a wide range of plant hosts. We aimed to study the metabolites produced at each stage of the nematode life cycle to understand its development. Metabolites of Meloidogyne incognita were extracted at egg, J2, J3, J4, and female stages and 110 metabolites with available standards were quantified using CE-TOF/MS. Analyses indicated abundance of stage-specific metabolites with the exception of J3 and J4 stages which shared similar metabolic profiles. The egg stage showed increased abundance in glycolysis and energy metabolism related metabolites while the J2 metabolites are associated with tissue formation, motility, and neurotransmission. The J3 and J4 stages indicated amino acid metabolism and urea cycle- related metabolites. The female stage was characterized with polyamine synthesis, antioxidant activity, and synthesis of reproduction related metabolites. Such metabolic profiling helps us understand the dynamic physiological changes related to each developmental stage of the root-knot nematode life cycle. MDPI 2017-06-24 /pmc/articles/PMC5535844/ /pubmed/28672815 http://dx.doi.org/10.3390/ijms18071351 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Subramanian, Parthiban
Oh, Byung-Ju
Mani, Vimalraj
Lee, Jae Kook
Lee, Chang-Muk
Sim, Joon-Soo
Koo, Ja Choon
Hahn, Bum-Soo
Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita
title Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita
title_full Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita
title_fullStr Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita
title_full_unstemmed Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita
title_short Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita
title_sort differential metabolic profiles during the developmental stages of plant-parasitic nematode meloidogyne incognita
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535844/
https://www.ncbi.nlm.nih.gov/pubmed/28672815
http://dx.doi.org/10.3390/ijms18071351
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