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Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita
Meloidogyne incognita is a common root-knot nematode with a wide range of plant hosts. We aimed to study the metabolites produced at each stage of the nematode life cycle to understand its development. Metabolites of Meloidogyne incognita were extracted at egg, J2, J3, J4, and female stages and 110...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535844/ https://www.ncbi.nlm.nih.gov/pubmed/28672815 http://dx.doi.org/10.3390/ijms18071351 |
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author | Subramanian, Parthiban Oh, Byung-Ju Mani, Vimalraj Lee, Jae Kook Lee, Chang-Muk Sim, Joon-Soo Koo, Ja Choon Hahn, Bum-Soo |
author_facet | Subramanian, Parthiban Oh, Byung-Ju Mani, Vimalraj Lee, Jae Kook Lee, Chang-Muk Sim, Joon-Soo Koo, Ja Choon Hahn, Bum-Soo |
author_sort | Subramanian, Parthiban |
collection | PubMed |
description | Meloidogyne incognita is a common root-knot nematode with a wide range of plant hosts. We aimed to study the metabolites produced at each stage of the nematode life cycle to understand its development. Metabolites of Meloidogyne incognita were extracted at egg, J2, J3, J4, and female stages and 110 metabolites with available standards were quantified using CE-TOF/MS. Analyses indicated abundance of stage-specific metabolites with the exception of J3 and J4 stages which shared similar metabolic profiles. The egg stage showed increased abundance in glycolysis and energy metabolism related metabolites while the J2 metabolites are associated with tissue formation, motility, and neurotransmission. The J3 and J4 stages indicated amino acid metabolism and urea cycle- related metabolites. The female stage was characterized with polyamine synthesis, antioxidant activity, and synthesis of reproduction related metabolites. Such metabolic profiling helps us understand the dynamic physiological changes related to each developmental stage of the root-knot nematode life cycle. |
format | Online Article Text |
id | pubmed-5535844 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-55358442017-08-04 Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita Subramanian, Parthiban Oh, Byung-Ju Mani, Vimalraj Lee, Jae Kook Lee, Chang-Muk Sim, Joon-Soo Koo, Ja Choon Hahn, Bum-Soo Int J Mol Sci Communication Meloidogyne incognita is a common root-knot nematode with a wide range of plant hosts. We aimed to study the metabolites produced at each stage of the nematode life cycle to understand its development. Metabolites of Meloidogyne incognita were extracted at egg, J2, J3, J4, and female stages and 110 metabolites with available standards were quantified using CE-TOF/MS. Analyses indicated abundance of stage-specific metabolites with the exception of J3 and J4 stages which shared similar metabolic profiles. The egg stage showed increased abundance in glycolysis and energy metabolism related metabolites while the J2 metabolites are associated with tissue formation, motility, and neurotransmission. The J3 and J4 stages indicated amino acid metabolism and urea cycle- related metabolites. The female stage was characterized with polyamine synthesis, antioxidant activity, and synthesis of reproduction related metabolites. Such metabolic profiling helps us understand the dynamic physiological changes related to each developmental stage of the root-knot nematode life cycle. MDPI 2017-06-24 /pmc/articles/PMC5535844/ /pubmed/28672815 http://dx.doi.org/10.3390/ijms18071351 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Subramanian, Parthiban Oh, Byung-Ju Mani, Vimalraj Lee, Jae Kook Lee, Chang-Muk Sim, Joon-Soo Koo, Ja Choon Hahn, Bum-Soo Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita |
title | Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita |
title_full | Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita |
title_fullStr | Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita |
title_full_unstemmed | Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita |
title_short | Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita |
title_sort | differential metabolic profiles during the developmental stages of plant-parasitic nematode meloidogyne incognita |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535844/ https://www.ncbi.nlm.nih.gov/pubmed/28672815 http://dx.doi.org/10.3390/ijms18071351 |
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