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Analysis of Hypericin-Mediated Effects and Implications for Targeted Photodynamic Therapy

The phototoxic effect of hypericin can be utilized for Photodynamic Therapy (PDT) of cancer. After intravenous application and systemic distribution of the drug in the patient’s body, the tumor site is exposed to light. Subsequently, toxic reactive oxygen species (ROS) are generated, inducing tumor...

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Autores principales: Mühleisen, Laura, Alev, Magdalena, Unterweger, Harald, Subatzus, Daniel, Pöttler, Marina, Friedrich, Ralf P., Alexiou, Christoph, Janko, Christina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535881/
https://www.ncbi.nlm.nih.gov/pubmed/28661430
http://dx.doi.org/10.3390/ijms18071388
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author Mühleisen, Laura
Alev, Magdalena
Unterweger, Harald
Subatzus, Daniel
Pöttler, Marina
Friedrich, Ralf P.
Alexiou, Christoph
Janko, Christina
author_facet Mühleisen, Laura
Alev, Magdalena
Unterweger, Harald
Subatzus, Daniel
Pöttler, Marina
Friedrich, Ralf P.
Alexiou, Christoph
Janko, Christina
author_sort Mühleisen, Laura
collection PubMed
description The phototoxic effect of hypericin can be utilized for Photodynamic Therapy (PDT) of cancer. After intravenous application and systemic distribution of the drug in the patient’s body, the tumor site is exposed to light. Subsequently, toxic reactive oxygen species (ROS) are generated, inducing tumor cell death. To prevent unwanted activation of the drug in other regions of the body, patients have to avoid light during and after the treatment cycles, consequently impairing quality of life. Here, we characterize toxicity and hypericin-mediated effects on cancer cells in vitro and confirm that its effect clearly depends on concentration and illumination time. To reduce side effects and to increase therapy success, selective accumulation of hypericin in the tumor region is a promising solution. Loading hypericin on superparamagnetic iron oxide nanoparticles (SPIONs) and guiding them to the desired place using an external magnetic field might accomplish this task (referred to as Magnetic Drug Targeting (MDT)). Thus, using a double targeting strategy, namely magnetic accumulation and laser induced photoactivation, might improve treatment effectivity as well as specificity and reduce toxic side effects in future clinical applications.
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spelling pubmed-55358812017-08-04 Analysis of Hypericin-Mediated Effects and Implications for Targeted Photodynamic Therapy Mühleisen, Laura Alev, Magdalena Unterweger, Harald Subatzus, Daniel Pöttler, Marina Friedrich, Ralf P. Alexiou, Christoph Janko, Christina Int J Mol Sci Article The phototoxic effect of hypericin can be utilized for Photodynamic Therapy (PDT) of cancer. After intravenous application and systemic distribution of the drug in the patient’s body, the tumor site is exposed to light. Subsequently, toxic reactive oxygen species (ROS) are generated, inducing tumor cell death. To prevent unwanted activation of the drug in other regions of the body, patients have to avoid light during and after the treatment cycles, consequently impairing quality of life. Here, we characterize toxicity and hypericin-mediated effects on cancer cells in vitro and confirm that its effect clearly depends on concentration and illumination time. To reduce side effects and to increase therapy success, selective accumulation of hypericin in the tumor region is a promising solution. Loading hypericin on superparamagnetic iron oxide nanoparticles (SPIONs) and guiding them to the desired place using an external magnetic field might accomplish this task (referred to as Magnetic Drug Targeting (MDT)). Thus, using a double targeting strategy, namely magnetic accumulation and laser induced photoactivation, might improve treatment effectivity as well as specificity and reduce toxic side effects in future clinical applications. MDPI 2017-06-29 /pmc/articles/PMC5535881/ /pubmed/28661430 http://dx.doi.org/10.3390/ijms18071388 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mühleisen, Laura
Alev, Magdalena
Unterweger, Harald
Subatzus, Daniel
Pöttler, Marina
Friedrich, Ralf P.
Alexiou, Christoph
Janko, Christina
Analysis of Hypericin-Mediated Effects and Implications for Targeted Photodynamic Therapy
title Analysis of Hypericin-Mediated Effects and Implications for Targeted Photodynamic Therapy
title_full Analysis of Hypericin-Mediated Effects and Implications for Targeted Photodynamic Therapy
title_fullStr Analysis of Hypericin-Mediated Effects and Implications for Targeted Photodynamic Therapy
title_full_unstemmed Analysis of Hypericin-Mediated Effects and Implications for Targeted Photodynamic Therapy
title_short Analysis of Hypericin-Mediated Effects and Implications for Targeted Photodynamic Therapy
title_sort analysis of hypericin-mediated effects and implications for targeted photodynamic therapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535881/
https://www.ncbi.nlm.nih.gov/pubmed/28661430
http://dx.doi.org/10.3390/ijms18071388
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