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Transgenic Strategies for Sparse but Strong Expression of Genetically Encoded Voltage and Calcium Indicators
Rapidly progressing development of optogenetic tools, particularly genetically encoded optical indicators, enables monitoring activities of neuronal circuits of identified cell populations in longitudinal in vivo studies. Recently developed advanced transgenic approaches achieve high levels of indic...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535952/ https://www.ncbi.nlm.nih.gov/pubmed/28686207 http://dx.doi.org/10.3390/ijms18071461 |
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author | Song, Chenchen Do, Quyen B. Antic, Srdjan D. Knöpfel, Thomas |
author_facet | Song, Chenchen Do, Quyen B. Antic, Srdjan D. Knöpfel, Thomas |
author_sort | Song, Chenchen |
collection | PubMed |
description | Rapidly progressing development of optogenetic tools, particularly genetically encoded optical indicators, enables monitoring activities of neuronal circuits of identified cell populations in longitudinal in vivo studies. Recently developed advanced transgenic approaches achieve high levels of indicator expression. However, targeting non-sparse cell populations leads to dense expression patterns such that optical signals from neuronal processes cannot be allocated to individual neurons. This issue is particularly pertinent for the use of genetically encoded voltage indicators whose membrane-delimited signals arise largely from the neuropil where dendritic and axonal membranes of many cells intermingle. Here we address this need for sparse but strong expression of genetically encoded optical indicators using a titratable recombination-activated transgene transcription to achieve a Golgi staining-type indicator expression pattern in vivo. Using different transgenic strategies, we also illustrate that co-expression of genetically encoded voltage and calcium indicators can be achieved in vivo for studying neuronal circuit input–output relationships. |
format | Online Article Text |
id | pubmed-5535952 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-55359522017-08-04 Transgenic Strategies for Sparse but Strong Expression of Genetically Encoded Voltage and Calcium Indicators Song, Chenchen Do, Quyen B. Antic, Srdjan D. Knöpfel, Thomas Int J Mol Sci Article Rapidly progressing development of optogenetic tools, particularly genetically encoded optical indicators, enables monitoring activities of neuronal circuits of identified cell populations in longitudinal in vivo studies. Recently developed advanced transgenic approaches achieve high levels of indicator expression. However, targeting non-sparse cell populations leads to dense expression patterns such that optical signals from neuronal processes cannot be allocated to individual neurons. This issue is particularly pertinent for the use of genetically encoded voltage indicators whose membrane-delimited signals arise largely from the neuropil where dendritic and axonal membranes of many cells intermingle. Here we address this need for sparse but strong expression of genetically encoded optical indicators using a titratable recombination-activated transgene transcription to achieve a Golgi staining-type indicator expression pattern in vivo. Using different transgenic strategies, we also illustrate that co-expression of genetically encoded voltage and calcium indicators can be achieved in vivo for studying neuronal circuit input–output relationships. MDPI 2017-07-07 /pmc/articles/PMC5535952/ /pubmed/28686207 http://dx.doi.org/10.3390/ijms18071461 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Song, Chenchen Do, Quyen B. Antic, Srdjan D. Knöpfel, Thomas Transgenic Strategies for Sparse but Strong Expression of Genetically Encoded Voltage and Calcium Indicators |
title | Transgenic Strategies for Sparse but Strong Expression of Genetically Encoded Voltage and Calcium Indicators |
title_full | Transgenic Strategies for Sparse but Strong Expression of Genetically Encoded Voltage and Calcium Indicators |
title_fullStr | Transgenic Strategies for Sparse but Strong Expression of Genetically Encoded Voltage and Calcium Indicators |
title_full_unstemmed | Transgenic Strategies for Sparse but Strong Expression of Genetically Encoded Voltage and Calcium Indicators |
title_short | Transgenic Strategies for Sparse but Strong Expression of Genetically Encoded Voltage and Calcium Indicators |
title_sort | transgenic strategies for sparse but strong expression of genetically encoded voltage and calcium indicators |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535952/ https://www.ncbi.nlm.nih.gov/pubmed/28686207 http://dx.doi.org/10.3390/ijms18071461 |
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