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The Prostate Cancer Cells Resistant to Docetaxel as in vitro Model for Discovering MicroRNAs Predictive of the Onset of Docetaxel Resistance
On the grounds that miRNAs present in the blood of prostate cancer (PCa) patients are released in the growth medium by PCa cells, it is conceivable that PCa cells resistant to docetaxel (DCT) (DCT(R)) will release miRNAs that may be found in PCa patients under DCT therapy if resistant PCa cells appe...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5536002/ https://www.ncbi.nlm.nih.gov/pubmed/28703747 http://dx.doi.org/10.3390/ijms18071512 |
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author | Bascetta, Lorenzo Oliviero, Arianna D’Aurizio, Romina Evangelista, Monica Mercatanti, Alberto Pellegrini, Marco Marrocolo, Francesca Bracarda, Sergio Rizzo, Milena |
author_facet | Bascetta, Lorenzo Oliviero, Arianna D’Aurizio, Romina Evangelista, Monica Mercatanti, Alberto Pellegrini, Marco Marrocolo, Francesca Bracarda, Sergio Rizzo, Milena |
author_sort | Bascetta, Lorenzo |
collection | PubMed |
description | On the grounds that miRNAs present in the blood of prostate cancer (PCa) patients are released in the growth medium by PCa cells, it is conceivable that PCa cells resistant to docetaxel (DCT) (DCT(R)) will release miRNAs that may be found in PCa patients under DCT therapy if resistant PCa cells appear. We isolated DCT(R) clones respectively from 22Rv1 and DU-145 PCa cell lines and performed through next-generation sequencing (NGS) the miRNAs profiles of the released miRNAs. The analysis of the NGS data identified 105 and 1 miRNAs which were differentially released in the growth medium of the 22Rv1/DCT(R) and DU-145/DCT(R) clones, respectively. Using additional filters, we selected 12 and 1 miRNA more released by all 22Rv1/DCT(R) and DU-145/DCT(R) clones, respectively. Moreover, we showed that 6 of them were more represented in the growth medium of the DCT(R) cells than the ones of DCT-treated cells. We speculated that they have the pre-requisite to be tested as predictive biomarkers of the DCT resistance in PCa patients under DCT therapy. We propose the utilization of clones resistant to a given drug as in vitro model to identify the differentially released miRNAs, which in perspective could be tested as predictive biomarkers of drug resistance in tumor patients under therapy. |
format | Online Article Text |
id | pubmed-5536002 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-55360022017-08-04 The Prostate Cancer Cells Resistant to Docetaxel as in vitro Model for Discovering MicroRNAs Predictive of the Onset of Docetaxel Resistance Bascetta, Lorenzo Oliviero, Arianna D’Aurizio, Romina Evangelista, Monica Mercatanti, Alberto Pellegrini, Marco Marrocolo, Francesca Bracarda, Sergio Rizzo, Milena Int J Mol Sci Article On the grounds that miRNAs present in the blood of prostate cancer (PCa) patients are released in the growth medium by PCa cells, it is conceivable that PCa cells resistant to docetaxel (DCT) (DCT(R)) will release miRNAs that may be found in PCa patients under DCT therapy if resistant PCa cells appear. We isolated DCT(R) clones respectively from 22Rv1 and DU-145 PCa cell lines and performed through next-generation sequencing (NGS) the miRNAs profiles of the released miRNAs. The analysis of the NGS data identified 105 and 1 miRNAs which were differentially released in the growth medium of the 22Rv1/DCT(R) and DU-145/DCT(R) clones, respectively. Using additional filters, we selected 12 and 1 miRNA more released by all 22Rv1/DCT(R) and DU-145/DCT(R) clones, respectively. Moreover, we showed that 6 of them were more represented in the growth medium of the DCT(R) cells than the ones of DCT-treated cells. We speculated that they have the pre-requisite to be tested as predictive biomarkers of the DCT resistance in PCa patients under DCT therapy. We propose the utilization of clones resistant to a given drug as in vitro model to identify the differentially released miRNAs, which in perspective could be tested as predictive biomarkers of drug resistance in tumor patients under therapy. MDPI 2017-07-13 /pmc/articles/PMC5536002/ /pubmed/28703747 http://dx.doi.org/10.3390/ijms18071512 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bascetta, Lorenzo Oliviero, Arianna D’Aurizio, Romina Evangelista, Monica Mercatanti, Alberto Pellegrini, Marco Marrocolo, Francesca Bracarda, Sergio Rizzo, Milena The Prostate Cancer Cells Resistant to Docetaxel as in vitro Model for Discovering MicroRNAs Predictive of the Onset of Docetaxel Resistance |
title | The Prostate Cancer Cells Resistant to Docetaxel as in vitro Model for Discovering MicroRNAs Predictive of the Onset of Docetaxel Resistance |
title_full | The Prostate Cancer Cells Resistant to Docetaxel as in vitro Model for Discovering MicroRNAs Predictive of the Onset of Docetaxel Resistance |
title_fullStr | The Prostate Cancer Cells Resistant to Docetaxel as in vitro Model for Discovering MicroRNAs Predictive of the Onset of Docetaxel Resistance |
title_full_unstemmed | The Prostate Cancer Cells Resistant to Docetaxel as in vitro Model for Discovering MicroRNAs Predictive of the Onset of Docetaxel Resistance |
title_short | The Prostate Cancer Cells Resistant to Docetaxel as in vitro Model for Discovering MicroRNAs Predictive of the Onset of Docetaxel Resistance |
title_sort | prostate cancer cells resistant to docetaxel as in vitro model for discovering micrornas predictive of the onset of docetaxel resistance |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5536002/ https://www.ncbi.nlm.nih.gov/pubmed/28703747 http://dx.doi.org/10.3390/ijms18071512 |
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