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Functional Implications of MicroRNAs in Crohn’s Disease Revealed by Integrating MicroRNA and Messenger RNA Expression Profiling

Crohn’s disease (CD) is a debilitating inflammatory bowel disease (IBD) that emerges due to the influence of genetic and environmental factors. microRNAs (miRNAs) have been identified in the tissue and sera of IBD patients and may play an important role in the induction of IBD. Our study aimed to id...

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Autores principales: Palmieri, Orazio, Creanza, Teresa Maria, Bossa, Fabrizio, Latiano, Tiziana, Corritore, Giuseppe, Palumbo, Orazio, Martino, Giuseppina, Biscaglia, Giuseppe, Scimeca, Daniela, Carella, Massimo, Ancona, Nicola, Andriulli, Angelo, Latiano, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5536068/
https://www.ncbi.nlm.nih.gov/pubmed/28726756
http://dx.doi.org/10.3390/ijms18071580
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author Palmieri, Orazio
Creanza, Teresa Maria
Bossa, Fabrizio
Latiano, Tiziana
Corritore, Giuseppe
Palumbo, Orazio
Martino, Giuseppina
Biscaglia, Giuseppe
Scimeca, Daniela
Carella, Massimo
Ancona, Nicola
Andriulli, Angelo
Latiano, Anna
author_facet Palmieri, Orazio
Creanza, Teresa Maria
Bossa, Fabrizio
Latiano, Tiziana
Corritore, Giuseppe
Palumbo, Orazio
Martino, Giuseppina
Biscaglia, Giuseppe
Scimeca, Daniela
Carella, Massimo
Ancona, Nicola
Andriulli, Angelo
Latiano, Anna
author_sort Palmieri, Orazio
collection PubMed
description Crohn’s disease (CD) is a debilitating inflammatory bowel disease (IBD) that emerges due to the influence of genetic and environmental factors. microRNAs (miRNAs) have been identified in the tissue and sera of IBD patients and may play an important role in the induction of IBD. Our study aimed to identify differentially expressed miRNAs and miRNAs with the ability to alter transcriptome activity by comparing inflamed tissue samples with their non-inflamed counterparts. We studied changes in miRNA–mRNA interactions associated with CD by examining their differential co-expression relative to normal mucosa from the same patients. Correlation changes between the two conditions were incorporated into scores of predefined gene sets to identify biological processes with altered miRNA-mediated control. Our study identified 28 miRNAs differentially expressed (p-values < 0.01), of which 14 are up-regulated. Notably, our differential co-expression analysis highlights microRNAs (i.e., miR-4284, miR-3194 and miR-21) that have known functional interactions with key mechanisms implicated in IBD. Most of these miRNAs cannot be detected by differential expression analysis that do not take into account miRNA–mRNA interactions. The identification of differential miRNA–mRNA co-expression patterns will facilitate the investigation of the miRNA-mediated molecular mechanisms underlying CD pathogenesis and could suggest novel drug targets for validation.
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spelling pubmed-55360682017-08-04 Functional Implications of MicroRNAs in Crohn’s Disease Revealed by Integrating MicroRNA and Messenger RNA Expression Profiling Palmieri, Orazio Creanza, Teresa Maria Bossa, Fabrizio Latiano, Tiziana Corritore, Giuseppe Palumbo, Orazio Martino, Giuseppina Biscaglia, Giuseppe Scimeca, Daniela Carella, Massimo Ancona, Nicola Andriulli, Angelo Latiano, Anna Int J Mol Sci Article Crohn’s disease (CD) is a debilitating inflammatory bowel disease (IBD) that emerges due to the influence of genetic and environmental factors. microRNAs (miRNAs) have been identified in the tissue and sera of IBD patients and may play an important role in the induction of IBD. Our study aimed to identify differentially expressed miRNAs and miRNAs with the ability to alter transcriptome activity by comparing inflamed tissue samples with their non-inflamed counterparts. We studied changes in miRNA–mRNA interactions associated with CD by examining their differential co-expression relative to normal mucosa from the same patients. Correlation changes between the two conditions were incorporated into scores of predefined gene sets to identify biological processes with altered miRNA-mediated control. Our study identified 28 miRNAs differentially expressed (p-values < 0.01), of which 14 are up-regulated. Notably, our differential co-expression analysis highlights microRNAs (i.e., miR-4284, miR-3194 and miR-21) that have known functional interactions with key mechanisms implicated in IBD. Most of these miRNAs cannot be detected by differential expression analysis that do not take into account miRNA–mRNA interactions. The identification of differential miRNA–mRNA co-expression patterns will facilitate the investigation of the miRNA-mediated molecular mechanisms underlying CD pathogenesis and could suggest novel drug targets for validation. MDPI 2017-07-20 /pmc/articles/PMC5536068/ /pubmed/28726756 http://dx.doi.org/10.3390/ijms18071580 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Palmieri, Orazio
Creanza, Teresa Maria
Bossa, Fabrizio
Latiano, Tiziana
Corritore, Giuseppe
Palumbo, Orazio
Martino, Giuseppina
Biscaglia, Giuseppe
Scimeca, Daniela
Carella, Massimo
Ancona, Nicola
Andriulli, Angelo
Latiano, Anna
Functional Implications of MicroRNAs in Crohn’s Disease Revealed by Integrating MicroRNA and Messenger RNA Expression Profiling
title Functional Implications of MicroRNAs in Crohn’s Disease Revealed by Integrating MicroRNA and Messenger RNA Expression Profiling
title_full Functional Implications of MicroRNAs in Crohn’s Disease Revealed by Integrating MicroRNA and Messenger RNA Expression Profiling
title_fullStr Functional Implications of MicroRNAs in Crohn’s Disease Revealed by Integrating MicroRNA and Messenger RNA Expression Profiling
title_full_unstemmed Functional Implications of MicroRNAs in Crohn’s Disease Revealed by Integrating MicroRNA and Messenger RNA Expression Profiling
title_short Functional Implications of MicroRNAs in Crohn’s Disease Revealed by Integrating MicroRNA and Messenger RNA Expression Profiling
title_sort functional implications of micrornas in crohn’s disease revealed by integrating microrna and messenger rna expression profiling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5536068/
https://www.ncbi.nlm.nih.gov/pubmed/28726756
http://dx.doi.org/10.3390/ijms18071580
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