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Short-term application of dexamethasone on stem cells derived from human gingiva reduces the expression of RUNX2 and β-catenin

OBJECTIVE: Next-generation sequencing was performed to evaluate the effects of short-term application of dexamethasone on human gingiva-derived mesenchymal stem cells. METHODS: Human gingiva-derived stem cells were treated with a final concentration of 10(−7 )M dexamethasone and the same concentrati...

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Autores principales: Kim, Bo-Bae, Kim, Minji, Park, Yun-Hee, Ko, Youngkyung, Park, Jun-Beom
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5536397/
https://www.ncbi.nlm.nih.gov/pubmed/28459354
http://dx.doi.org/10.1177/0300060517701035
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author Kim, Bo-Bae
Kim, Minji
Park, Yun-Hee
Ko, Youngkyung
Park, Jun-Beom
author_facet Kim, Bo-Bae
Kim, Minji
Park, Yun-Hee
Ko, Youngkyung
Park, Jun-Beom
author_sort Kim, Bo-Bae
collection PubMed
description OBJECTIVE: Next-generation sequencing was performed to evaluate the effects of short-term application of dexamethasone on human gingiva-derived mesenchymal stem cells. METHODS: Human gingiva-derived stem cells were treated with a final concentration of 10(−7 )M dexamethasone and the same concentration of vehicle control. This was followed by mRNA sequencing and data analysis, gene ontology and pathway analysis, quantitative real-time polymerase chain reaction of mRNA, and western blot analysis of RUNX2 and β-catenin. RESULTS: In total, 26,364 mRNAs were differentially expressed. Comparison of the results of dexamethasone versus control at 2 hours revealed that 7 mRNAs were upregulated and 25 mRNAs were downregulated. The application of dexamethasone reduced the expression of RUNX2 and β-catenin in human gingiva-derived mesenchymal stem cells. CONCLUSION: The effects of dexamethasone on stem cells were evaluated with mRNA sequencing, and validation of the expression was performed with qualitative real-time polymerase chain reaction and western blot analysis. The results of this study can provide new insights into the role of mRNA sequencing in maxillofacial areas.
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spelling pubmed-55363972017-10-03 Short-term application of dexamethasone on stem cells derived from human gingiva reduces the expression of RUNX2 and β-catenin Kim, Bo-Bae Kim, Minji Park, Yun-Hee Ko, Youngkyung Park, Jun-Beom J Int Med Res Research Reports OBJECTIVE: Next-generation sequencing was performed to evaluate the effects of short-term application of dexamethasone on human gingiva-derived mesenchymal stem cells. METHODS: Human gingiva-derived stem cells were treated with a final concentration of 10(−7 )M dexamethasone and the same concentration of vehicle control. This was followed by mRNA sequencing and data analysis, gene ontology and pathway analysis, quantitative real-time polymerase chain reaction of mRNA, and western blot analysis of RUNX2 and β-catenin. RESULTS: In total, 26,364 mRNAs were differentially expressed. Comparison of the results of dexamethasone versus control at 2 hours revealed that 7 mRNAs were upregulated and 25 mRNAs were downregulated. The application of dexamethasone reduced the expression of RUNX2 and β-catenin in human gingiva-derived mesenchymal stem cells. CONCLUSION: The effects of dexamethasone on stem cells were evaluated with mRNA sequencing, and validation of the expression was performed with qualitative real-time polymerase chain reaction and western blot analysis. The results of this study can provide new insights into the role of mRNA sequencing in maxillofacial areas. SAGE Publications 2017-05-01 2017-06 /pmc/articles/PMC5536397/ /pubmed/28459354 http://dx.doi.org/10.1177/0300060517701035 Text en © The Author(s) 2017 http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Research Reports
Kim, Bo-Bae
Kim, Minji
Park, Yun-Hee
Ko, Youngkyung
Park, Jun-Beom
Short-term application of dexamethasone on stem cells derived from human gingiva reduces the expression of RUNX2 and β-catenin
title Short-term application of dexamethasone on stem cells derived from human gingiva reduces the expression of RUNX2 and β-catenin
title_full Short-term application of dexamethasone on stem cells derived from human gingiva reduces the expression of RUNX2 and β-catenin
title_fullStr Short-term application of dexamethasone on stem cells derived from human gingiva reduces the expression of RUNX2 and β-catenin
title_full_unstemmed Short-term application of dexamethasone on stem cells derived from human gingiva reduces the expression of RUNX2 and β-catenin
title_short Short-term application of dexamethasone on stem cells derived from human gingiva reduces the expression of RUNX2 and β-catenin
title_sort short-term application of dexamethasone on stem cells derived from human gingiva reduces the expression of runx2 and β-catenin
topic Research Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5536397/
https://www.ncbi.nlm.nih.gov/pubmed/28459354
http://dx.doi.org/10.1177/0300060517701035
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