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Effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase C-independent pathway
OBJECTIVE: This study was performed to investigate the effect of parathyroid hormone (PTH) on healing in osteoporotic fractures via a phospholipase C (PLC)-independent pathway and explore the mechanism of PTH-mediated bone formation. METHODS: Ninety-six 12-week-old C57BL/6J female mice underwent bil...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5536420/ https://www.ncbi.nlm.nih.gov/pubmed/28534698 http://dx.doi.org/10.1177/0300060517707075 |
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author | Li, Wei-Long Yu, Xiao Huang, Zhi-Ping Pang, Qing-Jiang |
author_facet | Li, Wei-Long Yu, Xiao Huang, Zhi-Ping Pang, Qing-Jiang |
author_sort | Li, Wei-Long |
collection | PubMed |
description | OBJECTIVE: This study was performed to investigate the effect of parathyroid hormone (PTH) on healing in osteoporotic fractures via a phospholipase C (PLC)-independent pathway and explore the mechanism of PTH-mediated bone formation. METHODS: Ninety-six 12-week-old C57BL/6J female mice underwent bilateral ovariectomy. One month later, the lower third of the femur was fractured and the mice were treated using saline, PTH(1-28), PTH(1-34), zoledronic acid (ZA), PTH(1-28)+ZA, and PTH(1-34)+ZA. The mice were killed at weeks 2 and 4 in each group. Biomechanical testing and micro-computed tomography were performed. RESULTS: The formation and strength of the callus increased in all but the saline group. The mice treated with PTH(1-34) showed a significantly higher ultimate bending force, bending rigidity, bone mineral density, percent bone volume, and trabecular thickness than those treated with PTH(1-28). The PTH(1-34)+ZA group demonstrated the greatest improvements in the ultimate bending force, bending rigidity, bone mineral density, and relative bone volume. CONCLUSIONS: PTH can promote fracture healing and callus hardness in ovariectomized mice by increasing callus formation and reconstructing trabecular bone via a PLC-independent pathway. PTH combined with ZA has a cumulative effect on the healing of fractures in ovariectomized mice. |
format | Online Article Text |
id | pubmed-5536420 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-55364202017-10-03 Effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase C-independent pathway Li, Wei-Long Yu, Xiao Huang, Zhi-Ping Pang, Qing-Jiang J Int Med Res Clinical Reports OBJECTIVE: This study was performed to investigate the effect of parathyroid hormone (PTH) on healing in osteoporotic fractures via a phospholipase C (PLC)-independent pathway and explore the mechanism of PTH-mediated bone formation. METHODS: Ninety-six 12-week-old C57BL/6J female mice underwent bilateral ovariectomy. One month later, the lower third of the femur was fractured and the mice were treated using saline, PTH(1-28), PTH(1-34), zoledronic acid (ZA), PTH(1-28)+ZA, and PTH(1-34)+ZA. The mice were killed at weeks 2 and 4 in each group. Biomechanical testing and micro-computed tomography were performed. RESULTS: The formation and strength of the callus increased in all but the saline group. The mice treated with PTH(1-34) showed a significantly higher ultimate bending force, bending rigidity, bone mineral density, percent bone volume, and trabecular thickness than those treated with PTH(1-28). The PTH(1-34)+ZA group demonstrated the greatest improvements in the ultimate bending force, bending rigidity, bone mineral density, and relative bone volume. CONCLUSIONS: PTH can promote fracture healing and callus hardness in ovariectomized mice by increasing callus formation and reconstructing trabecular bone via a PLC-independent pathway. PTH combined with ZA has a cumulative effect on the healing of fractures in ovariectomized mice. SAGE Publications 2017-05-23 2017-06 /pmc/articles/PMC5536420/ /pubmed/28534698 http://dx.doi.org/10.1177/0300060517707075 Text en © The Author(s) 2017 http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Clinical Reports Li, Wei-Long Yu, Xiao Huang, Zhi-Ping Pang, Qing-Jiang Effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase C-independent pathway |
title | Effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase C-independent pathway |
title_full | Effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase C-independent pathway |
title_fullStr | Effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase C-independent pathway |
title_full_unstemmed | Effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase C-independent pathway |
title_short | Effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase C-independent pathway |
title_sort | effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase c-independent pathway |
topic | Clinical Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5536420/ https://www.ncbi.nlm.nih.gov/pubmed/28534698 http://dx.doi.org/10.1177/0300060517707075 |
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