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Effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase C-independent pathway

OBJECTIVE: This study was performed to investigate the effect of parathyroid hormone (PTH) on healing in osteoporotic fractures via a phospholipase C (PLC)-independent pathway and explore the mechanism of PTH-mediated bone formation. METHODS: Ninety-six 12-week-old C57BL/6J female mice underwent bil...

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Autores principales: Li, Wei-Long, Yu, Xiao, Huang, Zhi-Ping, Pang, Qing-Jiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5536420/
https://www.ncbi.nlm.nih.gov/pubmed/28534698
http://dx.doi.org/10.1177/0300060517707075
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author Li, Wei-Long
Yu, Xiao
Huang, Zhi-Ping
Pang, Qing-Jiang
author_facet Li, Wei-Long
Yu, Xiao
Huang, Zhi-Ping
Pang, Qing-Jiang
author_sort Li, Wei-Long
collection PubMed
description OBJECTIVE: This study was performed to investigate the effect of parathyroid hormone (PTH) on healing in osteoporotic fractures via a phospholipase C (PLC)-independent pathway and explore the mechanism of PTH-mediated bone formation. METHODS: Ninety-six 12-week-old C57BL/6J female mice underwent bilateral ovariectomy. One month later, the lower third of the femur was fractured and the mice were treated using saline, PTH(1-28), PTH(1-34), zoledronic acid (ZA), PTH(1-28)+ZA, and PTH(1-34)+ZA. The mice were killed at weeks 2 and 4 in each group. Biomechanical testing and micro-computed tomography were performed. RESULTS: The formation and strength of the callus increased in all but the saline group. The mice treated with PTH(1-34) showed a significantly higher ultimate bending force, bending rigidity, bone mineral density, percent bone volume, and trabecular thickness than those treated with PTH(1-28). The PTH(1-34)+ZA group demonstrated the greatest improvements in the ultimate bending force, bending rigidity, bone mineral density, and relative bone volume. CONCLUSIONS: PTH can promote fracture healing and callus hardness in ovariectomized mice by increasing callus formation and reconstructing trabecular bone via a PLC-independent pathway. PTH combined with ZA has a cumulative effect on the healing of fractures in ovariectomized mice.
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spelling pubmed-55364202017-10-03 Effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase C-independent pathway Li, Wei-Long Yu, Xiao Huang, Zhi-Ping Pang, Qing-Jiang J Int Med Res Clinical Reports OBJECTIVE: This study was performed to investigate the effect of parathyroid hormone (PTH) on healing in osteoporotic fractures via a phospholipase C (PLC)-independent pathway and explore the mechanism of PTH-mediated bone formation. METHODS: Ninety-six 12-week-old C57BL/6J female mice underwent bilateral ovariectomy. One month later, the lower third of the femur was fractured and the mice were treated using saline, PTH(1-28), PTH(1-34), zoledronic acid (ZA), PTH(1-28)+ZA, and PTH(1-34)+ZA. The mice were killed at weeks 2 and 4 in each group. Biomechanical testing and micro-computed tomography were performed. RESULTS: The formation and strength of the callus increased in all but the saline group. The mice treated with PTH(1-34) showed a significantly higher ultimate bending force, bending rigidity, bone mineral density, percent bone volume, and trabecular thickness than those treated with PTH(1-28). The PTH(1-34)+ZA group demonstrated the greatest improvements in the ultimate bending force, bending rigidity, bone mineral density, and relative bone volume. CONCLUSIONS: PTH can promote fracture healing and callus hardness in ovariectomized mice by increasing callus formation and reconstructing trabecular bone via a PLC-independent pathway. PTH combined with ZA has a cumulative effect on the healing of fractures in ovariectomized mice. SAGE Publications 2017-05-23 2017-06 /pmc/articles/PMC5536420/ /pubmed/28534698 http://dx.doi.org/10.1177/0300060517707075 Text en © The Author(s) 2017 http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Clinical Reports
Li, Wei-Long
Yu, Xiao
Huang, Zhi-Ping
Pang, Qing-Jiang
Effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase C-independent pathway
title Effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase C-independent pathway
title_full Effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase C-independent pathway
title_fullStr Effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase C-independent pathway
title_full_unstemmed Effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase C-independent pathway
title_short Effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase C-independent pathway
title_sort effect of parathyroid hormone on healing in osteoporotic fractures via a phospholipase c-independent pathway
topic Clinical Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5536420/
https://www.ncbi.nlm.nih.gov/pubmed/28534698
http://dx.doi.org/10.1177/0300060517707075
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