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Anti- trachea inflammatory effects of diosgenin from Dioscorea nipponica through interactions with glucocorticoid receptor α

Asthma is a heterogeneous disease characterized by symptoms of chronic inflammation and airway structural and functional changes. It affects about 300 million people worldwide and causes 250 000 deaths annually, but its symptoms can be greatly relieved by regular use of inhaled glucocorticoids (GCs)...

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Autores principales: Junchao, Yang, Zhen, Wang, Yuan, Wang, Liying, Xu, Libin, Jiang, Yuanhong, Zhu, Wei, Zhao, Ruilin, Chen, Lu, Zhai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5536614/
https://www.ncbi.nlm.nih.gov/pubmed/27913746
http://dx.doi.org/10.1177/0300060516676724
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author Junchao, Yang
Zhen, Wang
Yuan, Wang
Liying, Xu
Libin, Jiang
Yuanhong, Zhu
Wei, Zhao
Ruilin, Chen
Lu, Zhai
author_facet Junchao, Yang
Zhen, Wang
Yuan, Wang
Liying, Xu
Libin, Jiang
Yuanhong, Zhu
Wei, Zhao
Ruilin, Chen
Lu, Zhai
author_sort Junchao, Yang
collection PubMed
description Asthma is a heterogeneous disease characterized by symptoms of chronic inflammation and airway structural and functional changes. It affects about 300 million people worldwide and causes 250 000 deaths annually, but its symptoms can be greatly relieved by regular use of inhaled glucocorticoids (GCs). GCs exert their function through interacting with glucocorticoid receptors (GRs). Diosgenin is a naturally occurring steroidal saponin abundantly present in many medicinal plants, including Dioscorea nipponica, which shares a similar steroidal structure with GC. In this study, ovalbumin (OVA)-induced asthmatic mice and primary tracheal epithelial cells (TECs) were used as research models. ELISAs were applied to measure the secretion of TNF-α, IL-1β, and IL-6, while quantitative PCR and western blotting were applied to evaluate expression of GRs SLPI, TTP, GILZ, MKP-1, and NF-κB. Our data demonstrated that diosgenin suppressed the secretion of TNF-α, IL-1β, and IL-6 by enhancing the expression of GRs, SLPI, GILZ, and MKP-1, and inhibiting the expression of HSP70. These data provide some evidence on the molecular mechanism of diosgenin, which might facilitate its clinical applications.
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spelling pubmed-55366142017-10-03 Anti- trachea inflammatory effects of diosgenin from Dioscorea nipponica through interactions with glucocorticoid receptor α Junchao, Yang Zhen, Wang Yuan, Wang Liying, Xu Libin, Jiang Yuanhong, Zhu Wei, Zhao Ruilin, Chen Lu, Zhai J Int Med Res Research Reports Asthma is a heterogeneous disease characterized by symptoms of chronic inflammation and airway structural and functional changes. It affects about 300 million people worldwide and causes 250 000 deaths annually, but its symptoms can be greatly relieved by regular use of inhaled glucocorticoids (GCs). GCs exert their function through interacting with glucocorticoid receptors (GRs). Diosgenin is a naturally occurring steroidal saponin abundantly present in many medicinal plants, including Dioscorea nipponica, which shares a similar steroidal structure with GC. In this study, ovalbumin (OVA)-induced asthmatic mice and primary tracheal epithelial cells (TECs) were used as research models. ELISAs were applied to measure the secretion of TNF-α, IL-1β, and IL-6, while quantitative PCR and western blotting were applied to evaluate expression of GRs SLPI, TTP, GILZ, MKP-1, and NF-κB. Our data demonstrated that diosgenin suppressed the secretion of TNF-α, IL-1β, and IL-6 by enhancing the expression of GRs, SLPI, GILZ, and MKP-1, and inhibiting the expression of HSP70. These data provide some evidence on the molecular mechanism of diosgenin, which might facilitate its clinical applications. SAGE Publications 2016-12-02 2017-02 /pmc/articles/PMC5536614/ /pubmed/27913746 http://dx.doi.org/10.1177/0300060516676724 Text en © The Author(s) 2016 http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Research Reports
Junchao, Yang
Zhen, Wang
Yuan, Wang
Liying, Xu
Libin, Jiang
Yuanhong, Zhu
Wei, Zhao
Ruilin, Chen
Lu, Zhai
Anti- trachea inflammatory effects of diosgenin from Dioscorea nipponica through interactions with glucocorticoid receptor α
title Anti- trachea inflammatory effects of diosgenin from Dioscorea nipponica through interactions with glucocorticoid receptor α
title_full Anti- trachea inflammatory effects of diosgenin from Dioscorea nipponica through interactions with glucocorticoid receptor α
title_fullStr Anti- trachea inflammatory effects of diosgenin from Dioscorea nipponica through interactions with glucocorticoid receptor α
title_full_unstemmed Anti- trachea inflammatory effects of diosgenin from Dioscorea nipponica through interactions with glucocorticoid receptor α
title_short Anti- trachea inflammatory effects of diosgenin from Dioscorea nipponica through interactions with glucocorticoid receptor α
title_sort anti- trachea inflammatory effects of diosgenin from dioscorea nipponica through interactions with glucocorticoid receptor α
topic Research Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5536614/
https://www.ncbi.nlm.nih.gov/pubmed/27913746
http://dx.doi.org/10.1177/0300060516676724
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