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Taccaoside induces apoptosis in hepatocellular carcinoma cell lines
OBJECTIVE: Taccaoside, a steroidal saponin, has been shown to be cytotoxic, although the mechanism of cytotoxicity remains unclear. This study examined the effect of taccaoside on the human hepatocellular carcinoma (HCC) cell lines SMMC-7721 and Bel-7404. METHODS: The antiproliferative effect of tac...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5536763/ https://www.ncbi.nlm.nih.gov/pubmed/27856932 http://dx.doi.org/10.1177/0300060516672368 |
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author | Sun, Yuewen Qiu, Hanchen Ou, Mingchun Chen, Runli Liang, Gang |
author_facet | Sun, Yuewen Qiu, Hanchen Ou, Mingchun Chen, Runli Liang, Gang |
author_sort | Sun, Yuewen |
collection | PubMed |
description | OBJECTIVE: Taccaoside, a steroidal saponin, has been shown to be cytotoxic, although the mechanism of cytotoxicity remains unclear. This study examined the effect of taccaoside on the human hepatocellular carcinoma (HCC) cell lines SMMC-7721 and Bel-7404. METHODS: The antiproliferative effect of taccaoside were measured using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Cells were stained with Hoechst 33258 to observe morphology. Cell cycle and apoptosis were analysed by flow cytometry. Caspase activation was detected using specific assays, and PARP, Bax and Bcl-2 expression were analysed using western blotting. RESULTS: Taccaoside showed antiproliferative effect on HCC cell lines growth in a concentration- and time-dependent manner. Taccaoside arrested cell cycle in the G2/M phase and induced caspase-dependent apoptosis. Western blotting indicated that taccaoside upregulated Bax expression and downregulated Bcl-2 expression. PARP cleavage was observed following taccaoside treatment. CONCLUSIONS: This study showed that taccaoside may inhibit HCC cell proliferation by inducing apoptosis. |
format | Online Article Text |
id | pubmed-5536763 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-55367632017-10-03 Taccaoside induces apoptosis in hepatocellular carcinoma cell lines Sun, Yuewen Qiu, Hanchen Ou, Mingchun Chen, Runli Liang, Gang J Int Med Res Research Reports OBJECTIVE: Taccaoside, a steroidal saponin, has been shown to be cytotoxic, although the mechanism of cytotoxicity remains unclear. This study examined the effect of taccaoside on the human hepatocellular carcinoma (HCC) cell lines SMMC-7721 and Bel-7404. METHODS: The antiproliferative effect of taccaoside were measured using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Cells were stained with Hoechst 33258 to observe morphology. Cell cycle and apoptosis were analysed by flow cytometry. Caspase activation was detected using specific assays, and PARP, Bax and Bcl-2 expression were analysed using western blotting. RESULTS: Taccaoside showed antiproliferative effect on HCC cell lines growth in a concentration- and time-dependent manner. Taccaoside arrested cell cycle in the G2/M phase and induced caspase-dependent apoptosis. Western blotting indicated that taccaoside upregulated Bax expression and downregulated Bcl-2 expression. PARP cleavage was observed following taccaoside treatment. CONCLUSIONS: This study showed that taccaoside may inhibit HCC cell proliferation by inducing apoptosis. SAGE Publications 2016-11-17 2016-12 /pmc/articles/PMC5536763/ /pubmed/27856932 http://dx.doi.org/10.1177/0300060516672368 Text en © The Author(s) 2016 http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Research Reports Sun, Yuewen Qiu, Hanchen Ou, Mingchun Chen, Runli Liang, Gang Taccaoside induces apoptosis in hepatocellular carcinoma cell lines |
title | Taccaoside induces apoptosis in hepatocellular carcinoma cell lines |
title_full | Taccaoside induces apoptosis in hepatocellular carcinoma cell lines |
title_fullStr | Taccaoside induces apoptosis in hepatocellular carcinoma cell lines |
title_full_unstemmed | Taccaoside induces apoptosis in hepatocellular carcinoma cell lines |
title_short | Taccaoside induces apoptosis in hepatocellular carcinoma cell lines |
title_sort | taccaoside induces apoptosis in hepatocellular carcinoma cell lines |
topic | Research Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5536763/ https://www.ncbi.nlm.nih.gov/pubmed/27856932 http://dx.doi.org/10.1177/0300060516672368 |
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