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LncRNA TRERNA1 Function as an Enhancer of SNAI1 Promotes Gastric Cancer Metastasis by Regulating Epithelial-Mesenchymal Transition

Long noncoding RNA (lncRNA) has been implicated in cancer, but little is known about the role of lncRNAs as regulators of tumor metastasis. In the present study, we demonstrate that lncRNA TRERNA1 acts like an enhancer of SNAI1 to promote cell invasion and migration and to contribute to metastasis o...

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Autores principales: Wu, Huazhang, Hu, Ying, Liu, Xiufang, Song, Wei, Gong, Pihai, Zhang, Kun, Chen, Zhenxing, Zhou, Menghan, Shen, Xiaohui, Qian, Yanyan, Fan, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5537167/
https://www.ncbi.nlm.nih.gov/pubmed/28918030
http://dx.doi.org/10.1016/j.omtn.2017.06.021
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author Wu, Huazhang
Hu, Ying
Liu, Xiufang
Song, Wei
Gong, Pihai
Zhang, Kun
Chen, Zhenxing
Zhou, Menghan
Shen, Xiaohui
Qian, Yanyan
Fan, Hong
author_facet Wu, Huazhang
Hu, Ying
Liu, Xiufang
Song, Wei
Gong, Pihai
Zhang, Kun
Chen, Zhenxing
Zhou, Menghan
Shen, Xiaohui
Qian, Yanyan
Fan, Hong
author_sort Wu, Huazhang
collection PubMed
description Long noncoding RNA (lncRNA) has been implicated in cancer, but little is known about the role of lncRNAs as regulators of tumor metastasis. In the present study, we demonstrate that lncRNA TRERNA1 acts like an enhancer of SNAI1 to promote cell invasion and migration and to contribute to metastasis of gastric cancer (GC). TRERNA1 is significantly unregulated in GCs and GC cell lines. Increased TRERNA1 is positively correlated with lymph node metastasis of GCs. RNA immunoprecipitation (RIP) and chromatin immunoprecipitation (ChIP) assays revealed that TRERNA1 functions as a scaffold to recruit EZH2 to epigenetically silence epithelial-mesenchymal transition marker CDH1 by H3K27me3 of its promoter region. TRERNA1 knockdown markedly reduced GC cell migration, invasion, tumorigenicity, and metastasis. Depletion of TRERNA1 reduced cell metastasis of GCs in vivo. Taken together, our findings indicated that TRERNA1 serves as a critical effector in GC progression by regulating CDH1 at the transcription level. It is implied that TRERNA1/CDH1 is a new potential target for GC therapy.
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spelling pubmed-55371672017-08-08 LncRNA TRERNA1 Function as an Enhancer of SNAI1 Promotes Gastric Cancer Metastasis by Regulating Epithelial-Mesenchymal Transition Wu, Huazhang Hu, Ying Liu, Xiufang Song, Wei Gong, Pihai Zhang, Kun Chen, Zhenxing Zhou, Menghan Shen, Xiaohui Qian, Yanyan Fan, Hong Mol Ther Nucleic Acids Original Article Long noncoding RNA (lncRNA) has been implicated in cancer, but little is known about the role of lncRNAs as regulators of tumor metastasis. In the present study, we demonstrate that lncRNA TRERNA1 acts like an enhancer of SNAI1 to promote cell invasion and migration and to contribute to metastasis of gastric cancer (GC). TRERNA1 is significantly unregulated in GCs and GC cell lines. Increased TRERNA1 is positively correlated with lymph node metastasis of GCs. RNA immunoprecipitation (RIP) and chromatin immunoprecipitation (ChIP) assays revealed that TRERNA1 functions as a scaffold to recruit EZH2 to epigenetically silence epithelial-mesenchymal transition marker CDH1 by H3K27me3 of its promoter region. TRERNA1 knockdown markedly reduced GC cell migration, invasion, tumorigenicity, and metastasis. Depletion of TRERNA1 reduced cell metastasis of GCs in vivo. Taken together, our findings indicated that TRERNA1 serves as a critical effector in GC progression by regulating CDH1 at the transcription level. It is implied that TRERNA1/CDH1 is a new potential target for GC therapy. American Society of Gene & Cell Therapy 2017-07-03 /pmc/articles/PMC5537167/ /pubmed/28918030 http://dx.doi.org/10.1016/j.omtn.2017.06.021 Text en © 2017 The American Society of Gene and Cell Therapy. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Wu, Huazhang
Hu, Ying
Liu, Xiufang
Song, Wei
Gong, Pihai
Zhang, Kun
Chen, Zhenxing
Zhou, Menghan
Shen, Xiaohui
Qian, Yanyan
Fan, Hong
LncRNA TRERNA1 Function as an Enhancer of SNAI1 Promotes Gastric Cancer Metastasis by Regulating Epithelial-Mesenchymal Transition
title LncRNA TRERNA1 Function as an Enhancer of SNAI1 Promotes Gastric Cancer Metastasis by Regulating Epithelial-Mesenchymal Transition
title_full LncRNA TRERNA1 Function as an Enhancer of SNAI1 Promotes Gastric Cancer Metastasis by Regulating Epithelial-Mesenchymal Transition
title_fullStr LncRNA TRERNA1 Function as an Enhancer of SNAI1 Promotes Gastric Cancer Metastasis by Regulating Epithelial-Mesenchymal Transition
title_full_unstemmed LncRNA TRERNA1 Function as an Enhancer of SNAI1 Promotes Gastric Cancer Metastasis by Regulating Epithelial-Mesenchymal Transition
title_short LncRNA TRERNA1 Function as an Enhancer of SNAI1 Promotes Gastric Cancer Metastasis by Regulating Epithelial-Mesenchymal Transition
title_sort lncrna trerna1 function as an enhancer of snai1 promotes gastric cancer metastasis by regulating epithelial-mesenchymal transition
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5537167/
https://www.ncbi.nlm.nih.gov/pubmed/28918030
http://dx.doi.org/10.1016/j.omtn.2017.06.021
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