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Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat
Ocular and periocular traumatisms may result in loss of vision. Hypothermia provides a beneficial intervention for brain and heart conditions and, here, we study whether hypothermia can prevent retinal damage caused by traumatic neuropathy. Intraorbital optic nerve crush (IONC) or sham manipulation...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5537267/ https://www.ncbi.nlm.nih.gov/pubmed/28761115 http://dx.doi.org/10.1038/s41598-017-07294-6 |
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author | Rey-Funes, Manuel Larrayoz, Ignacio M. Contartese, Daniela S. Soliño, Manuel Sarotto, Anibal Bustelo, Martín Bruno, Martín Dorfman, Verónica B. Loidl, César F. Martínez, Alfredo |
author_facet | Rey-Funes, Manuel Larrayoz, Ignacio M. Contartese, Daniela S. Soliño, Manuel Sarotto, Anibal Bustelo, Martín Bruno, Martín Dorfman, Verónica B. Loidl, César F. Martínez, Alfredo |
author_sort | Rey-Funes, Manuel |
collection | PubMed |
description | Ocular and periocular traumatisms may result in loss of vision. Hypothermia provides a beneficial intervention for brain and heart conditions and, here, we study whether hypothermia can prevent retinal damage caused by traumatic neuropathy. Intraorbital optic nerve crush (IONC) or sham manipulation was applied to male rats. Some animals were subjected to hypothermia (8 °C) for 3 h following surgery. Thirty days later, animals were subjected to electroretinography and behavioral tests. IONC treatment resulted in amplitude reduction of the b-wave and oscillatory potentials of the electroretinogram, whereas the hypothermic treatment significantly (p < 0.05) reversed this process. Using a descending method of limits in a two-choice visual task apparatus, we demonstrated that hypothermia significantly (p < 0.001) preserved visual acuity. Furthermore, IONC-treated rats had a lower (p < 0.0001) number of retinal ganglion cells and a higher (p < 0.0001) number of TUNEL-positive cells than sham-operated controls. These numbers were significantly (p < 0.0001) corrected by hypothermic treatment. There was a significant (p < 0.001) increase of RNA-binding motif protein 3 (RBM3) and of BCL2 (p < 0.01) mRNA expression in the eyes exposed to hypothermia. In conclusion, hypothermia constitutes an efficacious treatment for traumatic vision-impairing conditions, and the cold-shock protein pathway may be involved in mediating the beneficial effects shown in the retina. |
format | Online Article Text |
id | pubmed-5537267 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55372672017-08-03 Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat Rey-Funes, Manuel Larrayoz, Ignacio M. Contartese, Daniela S. Soliño, Manuel Sarotto, Anibal Bustelo, Martín Bruno, Martín Dorfman, Verónica B. Loidl, César F. Martínez, Alfredo Sci Rep Article Ocular and periocular traumatisms may result in loss of vision. Hypothermia provides a beneficial intervention for brain and heart conditions and, here, we study whether hypothermia can prevent retinal damage caused by traumatic neuropathy. Intraorbital optic nerve crush (IONC) or sham manipulation was applied to male rats. Some animals were subjected to hypothermia (8 °C) for 3 h following surgery. Thirty days later, animals were subjected to electroretinography and behavioral tests. IONC treatment resulted in amplitude reduction of the b-wave and oscillatory potentials of the electroretinogram, whereas the hypothermic treatment significantly (p < 0.05) reversed this process. Using a descending method of limits in a two-choice visual task apparatus, we demonstrated that hypothermia significantly (p < 0.001) preserved visual acuity. Furthermore, IONC-treated rats had a lower (p < 0.0001) number of retinal ganglion cells and a higher (p < 0.0001) number of TUNEL-positive cells than sham-operated controls. These numbers were significantly (p < 0.0001) corrected by hypothermic treatment. There was a significant (p < 0.001) increase of RNA-binding motif protein 3 (RBM3) and of BCL2 (p < 0.01) mRNA expression in the eyes exposed to hypothermia. In conclusion, hypothermia constitutes an efficacious treatment for traumatic vision-impairing conditions, and the cold-shock protein pathway may be involved in mediating the beneficial effects shown in the retina. Nature Publishing Group UK 2017-07-31 /pmc/articles/PMC5537267/ /pubmed/28761115 http://dx.doi.org/10.1038/s41598-017-07294-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Rey-Funes, Manuel Larrayoz, Ignacio M. Contartese, Daniela S. Soliño, Manuel Sarotto, Anibal Bustelo, Martín Bruno, Martín Dorfman, Verónica B. Loidl, César F. Martínez, Alfredo Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat |
title | Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat |
title_full | Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat |
title_fullStr | Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat |
title_full_unstemmed | Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat |
title_short | Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat |
title_sort | hypothermia prevents retinal damage generated by optic nerve trauma in the rat |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5537267/ https://www.ncbi.nlm.nih.gov/pubmed/28761115 http://dx.doi.org/10.1038/s41598-017-07294-6 |
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