Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands

Aberrant expression of TNF family of cytokines has been linked to human diseases, and biologics targeting their signaling have become the best selling drugs globally. However, functional detection with labeled ligands for accurate detection of TNFR family of receptor-expressing target tissues or cel...

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Autores principales: Xu, Yang, Chang, Lingmo, Huang, Anliang, Liu, Xiaojun, Liu, Xinyu, Zhou, Hong, Liang, Joshua G., Liang, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5537357/
https://www.ncbi.nlm.nih.gov/pubmed/28761167
http://dx.doi.org/10.1038/s41598-017-06343-4
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author Xu, Yang
Chang, Lingmo
Huang, Anliang
Liu, Xiaojun
Liu, Xinyu
Zhou, Hong
Liang, Joshua G.
Liang, Peng
author_facet Xu, Yang
Chang, Lingmo
Huang, Anliang
Liu, Xiaojun
Liu, Xinyu
Zhou, Hong
Liang, Joshua G.
Liang, Peng
author_sort Xu, Yang
collection PubMed
description Aberrant expression of TNF family of cytokines has been linked to human diseases, and biologics targeting their signaling have become the best selling drugs globally. However, functional detection with labeled ligands for accurate detection of TNFR family of receptor-expressing target tissues or cell types remains to be developed. Here we show that TNF receptor family members are heat-stable and can be recognized both in vitro and in vivo by their ligands labeled with alkaline phosphatase. Such an approach may be used in lieu of antibodies for the identification of the cell types involved in receptor signaling during disease onset and progression.
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spelling pubmed-55373572017-08-03 Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands Xu, Yang Chang, Lingmo Huang, Anliang Liu, Xiaojun Liu, Xinyu Zhou, Hong Liang, Joshua G. Liang, Peng Sci Rep Article Aberrant expression of TNF family of cytokines has been linked to human diseases, and biologics targeting their signaling have become the best selling drugs globally. However, functional detection with labeled ligands for accurate detection of TNFR family of receptor-expressing target tissues or cell types remains to be developed. Here we show that TNF receptor family members are heat-stable and can be recognized both in vitro and in vivo by their ligands labeled with alkaline phosphatase. Such an approach may be used in lieu of antibodies for the identification of the cell types involved in receptor signaling during disease onset and progression. Nature Publishing Group UK 2017-07-31 /pmc/articles/PMC5537357/ /pubmed/28761167 http://dx.doi.org/10.1038/s41598-017-06343-4 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Xu, Yang
Chang, Lingmo
Huang, Anliang
Liu, Xiaojun
Liu, Xinyu
Zhou, Hong
Liang, Joshua G.
Liang, Peng
Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands
title Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands
title_full Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands
title_fullStr Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands
title_full_unstemmed Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands
title_short Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands
title_sort functional detection of tnf receptor family members by affinity-labeled ligands
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5537357/
https://www.ncbi.nlm.nih.gov/pubmed/28761167
http://dx.doi.org/10.1038/s41598-017-06343-4
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