Cargando…

DNA-Interacting Characteristics of the Archaeal Rudiviral Protein SIRV2_Gp1

Whereas the infection cycles of many bacterial and eukaryotic viruses have been characterized in detail, those of archaeal viruses remain largely unexplored. Recently, studies on a few model archaeal viruses such as SIRV2 (Sulfolobus islandicus rod-shaped virus) have revealed an unusual lysis mechan...

Descripción completa

Detalles Bibliográficos
Autores principales: Peeters, Eveline, Boon, Maarten, Rollie, Clare, Willaert, Ronnie G., Voet, Marleen, White, Malcolm F., Prangishvili, David, Lavigne, Rob, Quax, Tessa E. F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5537682/
https://www.ncbi.nlm.nih.gov/pubmed/28718834
http://dx.doi.org/10.3390/v9070190
_version_ 1783254228958969856
author Peeters, Eveline
Boon, Maarten
Rollie, Clare
Willaert, Ronnie G.
Voet, Marleen
White, Malcolm F.
Prangishvili, David
Lavigne, Rob
Quax, Tessa E. F.
author_facet Peeters, Eveline
Boon, Maarten
Rollie, Clare
Willaert, Ronnie G.
Voet, Marleen
White, Malcolm F.
Prangishvili, David
Lavigne, Rob
Quax, Tessa E. F.
author_sort Peeters, Eveline
collection PubMed
description Whereas the infection cycles of many bacterial and eukaryotic viruses have been characterized in detail, those of archaeal viruses remain largely unexplored. Recently, studies on a few model archaeal viruses such as SIRV2 (Sulfolobus islandicus rod-shaped virus) have revealed an unusual lysis mechanism that involves the formation of pyramidal egress structures on the host cell surface. To expand understanding of the infection cycle of SIRV2, we aimed to functionally characterize gp1, which is a SIRV2 gene with unknown function. The SIRV2_Gp1 protein is highly expressed during early stages of infection and it is the only protein that is encoded twice on the viral genome. It harbours a helix-turn-helix motif and was therefore hypothesized to bind DNA. The DNA-binding behavior of SIRV2_Gp1 was characterized with electrophoretic mobility shift assays and atomic force microscopy. We provide evidence that the protein interacts with DNA and that it forms large aggregates, thereby causing extreme condensation of the DNA. Furthermore, the N-terminal domain of the protein mediates toxicity to the viral host Sulfolobus. Our findings may lead to biotechnological applications, such as the development of a toxic peptide for the containment of pathogenic bacteria, and add to our understanding of the Rudiviral infection cycle.
format Online
Article
Text
id pubmed-5537682
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-55376822017-08-04 DNA-Interacting Characteristics of the Archaeal Rudiviral Protein SIRV2_Gp1 Peeters, Eveline Boon, Maarten Rollie, Clare Willaert, Ronnie G. Voet, Marleen White, Malcolm F. Prangishvili, David Lavigne, Rob Quax, Tessa E. F. Viruses Article Whereas the infection cycles of many bacterial and eukaryotic viruses have been characterized in detail, those of archaeal viruses remain largely unexplored. Recently, studies on a few model archaeal viruses such as SIRV2 (Sulfolobus islandicus rod-shaped virus) have revealed an unusual lysis mechanism that involves the formation of pyramidal egress structures on the host cell surface. To expand understanding of the infection cycle of SIRV2, we aimed to functionally characterize gp1, which is a SIRV2 gene with unknown function. The SIRV2_Gp1 protein is highly expressed during early stages of infection and it is the only protein that is encoded twice on the viral genome. It harbours a helix-turn-helix motif and was therefore hypothesized to bind DNA. The DNA-binding behavior of SIRV2_Gp1 was characterized with electrophoretic mobility shift assays and atomic force microscopy. We provide evidence that the protein interacts with DNA and that it forms large aggregates, thereby causing extreme condensation of the DNA. Furthermore, the N-terminal domain of the protein mediates toxicity to the viral host Sulfolobus. Our findings may lead to biotechnological applications, such as the development of a toxic peptide for the containment of pathogenic bacteria, and add to our understanding of the Rudiviral infection cycle. MDPI 2017-07-18 /pmc/articles/PMC5537682/ /pubmed/28718834 http://dx.doi.org/10.3390/v9070190 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Peeters, Eveline
Boon, Maarten
Rollie, Clare
Willaert, Ronnie G.
Voet, Marleen
White, Malcolm F.
Prangishvili, David
Lavigne, Rob
Quax, Tessa E. F.
DNA-Interacting Characteristics of the Archaeal Rudiviral Protein SIRV2_Gp1
title DNA-Interacting Characteristics of the Archaeal Rudiviral Protein SIRV2_Gp1
title_full DNA-Interacting Characteristics of the Archaeal Rudiviral Protein SIRV2_Gp1
title_fullStr DNA-Interacting Characteristics of the Archaeal Rudiviral Protein SIRV2_Gp1
title_full_unstemmed DNA-Interacting Characteristics of the Archaeal Rudiviral Protein SIRV2_Gp1
title_short DNA-Interacting Characteristics of the Archaeal Rudiviral Protein SIRV2_Gp1
title_sort dna-interacting characteristics of the archaeal rudiviral protein sirv2_gp1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5537682/
https://www.ncbi.nlm.nih.gov/pubmed/28718834
http://dx.doi.org/10.3390/v9070190
work_keys_str_mv AT peeterseveline dnainteractingcharacteristicsofthearchaealrudiviralproteinsirv2gp1
AT boonmaarten dnainteractingcharacteristicsofthearchaealrudiviralproteinsirv2gp1
AT rollieclare dnainteractingcharacteristicsofthearchaealrudiviralproteinsirv2gp1
AT willaertronnieg dnainteractingcharacteristicsofthearchaealrudiviralproteinsirv2gp1
AT voetmarleen dnainteractingcharacteristicsofthearchaealrudiviralproteinsirv2gp1
AT whitemalcolmf dnainteractingcharacteristicsofthearchaealrudiviralproteinsirv2gp1
AT prangishvilidavid dnainteractingcharacteristicsofthearchaealrudiviralproteinsirv2gp1
AT lavignerob dnainteractingcharacteristicsofthearchaealrudiviralproteinsirv2gp1
AT quaxtessaef dnainteractingcharacteristicsofthearchaealrudiviralproteinsirv2gp1