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Dose-Dependent Prebiotic Effect of Lactulose in a Computer-Controlled In Vitro Model of the Human Large Intestine
Lactulose, a disaccharide of galactose and fructose, used as a laxative or ammonia-lowering drug and as a functional food ingredient, enhances growth of Bifidobacterium and Lactobacillus at clinically relevant dosages. The prebiotic effect of subclinical dosages of Lactulose, however, remains to be...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5537881/ https://www.ncbi.nlm.nih.gov/pubmed/28718839 http://dx.doi.org/10.3390/nu9070767 |
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author | Bothe, Melanie K. Maathuis, Annet J. H. Bellmann, Susann van der Vossen, Jos M. B. M. Berressem, Dirk Koehler, Annalena Schwejda-Guettes, Susann Gaigg, Barbara Kuchinka-Koch, Angelika Stover, John F. |
author_facet | Bothe, Melanie K. Maathuis, Annet J. H. Bellmann, Susann van der Vossen, Jos M. B. M. Berressem, Dirk Koehler, Annalena Schwejda-Guettes, Susann Gaigg, Barbara Kuchinka-Koch, Angelika Stover, John F. |
author_sort | Bothe, Melanie K. |
collection | PubMed |
description | Lactulose, a disaccharide of galactose and fructose, used as a laxative or ammonia-lowering drug and as a functional food ingredient, enhances growth of Bifidobacterium and Lactobacillus at clinically relevant dosages. The prebiotic effect of subclinical dosages of Lactulose, however, remains to be elucidated. This study analyses changes in the microbiota and their metabolites after a 5 days Lactulose treatment using the TIM-2 system, a computer-controlled model of the proximal large intestine representing a complex, high density, metabolically active, anaerobic microbiota of human origin. Subclinical dosages of 2–5 g Lactulose were used. While 2 g Lactulose already increased the short-chain fatty acid levels of the intestinal content, 5 g Lactulose were required daily for 5 days in this study to exert the full beneficial prebiotic effect consisting of higher bacterial counts of Bifidobacterium, Lactobacillus, and Anaerostipes, a rise in acetate, butyrate and lactate, as well as a decrease in branched-chain fatty acids, pH (suggested by an increase in NaOH usage), and ammonia. |
format | Online Article Text |
id | pubmed-5537881 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-55378812017-08-04 Dose-Dependent Prebiotic Effect of Lactulose in a Computer-Controlled In Vitro Model of the Human Large Intestine Bothe, Melanie K. Maathuis, Annet J. H. Bellmann, Susann van der Vossen, Jos M. B. M. Berressem, Dirk Koehler, Annalena Schwejda-Guettes, Susann Gaigg, Barbara Kuchinka-Koch, Angelika Stover, John F. Nutrients Article Lactulose, a disaccharide of galactose and fructose, used as a laxative or ammonia-lowering drug and as a functional food ingredient, enhances growth of Bifidobacterium and Lactobacillus at clinically relevant dosages. The prebiotic effect of subclinical dosages of Lactulose, however, remains to be elucidated. This study analyses changes in the microbiota and their metabolites after a 5 days Lactulose treatment using the TIM-2 system, a computer-controlled model of the proximal large intestine representing a complex, high density, metabolically active, anaerobic microbiota of human origin. Subclinical dosages of 2–5 g Lactulose were used. While 2 g Lactulose already increased the short-chain fatty acid levels of the intestinal content, 5 g Lactulose were required daily for 5 days in this study to exert the full beneficial prebiotic effect consisting of higher bacterial counts of Bifidobacterium, Lactobacillus, and Anaerostipes, a rise in acetate, butyrate and lactate, as well as a decrease in branched-chain fatty acids, pH (suggested by an increase in NaOH usage), and ammonia. MDPI 2017-07-18 /pmc/articles/PMC5537881/ /pubmed/28718839 http://dx.doi.org/10.3390/nu9070767 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bothe, Melanie K. Maathuis, Annet J. H. Bellmann, Susann van der Vossen, Jos M. B. M. Berressem, Dirk Koehler, Annalena Schwejda-Guettes, Susann Gaigg, Barbara Kuchinka-Koch, Angelika Stover, John F. Dose-Dependent Prebiotic Effect of Lactulose in a Computer-Controlled In Vitro Model of the Human Large Intestine |
title | Dose-Dependent Prebiotic Effect of Lactulose in a Computer-Controlled In Vitro Model of the Human Large Intestine |
title_full | Dose-Dependent Prebiotic Effect of Lactulose in a Computer-Controlled In Vitro Model of the Human Large Intestine |
title_fullStr | Dose-Dependent Prebiotic Effect of Lactulose in a Computer-Controlled In Vitro Model of the Human Large Intestine |
title_full_unstemmed | Dose-Dependent Prebiotic Effect of Lactulose in a Computer-Controlled In Vitro Model of the Human Large Intestine |
title_short | Dose-Dependent Prebiotic Effect of Lactulose in a Computer-Controlled In Vitro Model of the Human Large Intestine |
title_sort | dose-dependent prebiotic effect of lactulose in a computer-controlled in vitro model of the human large intestine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5537881/ https://www.ncbi.nlm.nih.gov/pubmed/28718839 http://dx.doi.org/10.3390/nu9070767 |
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