Cargando…

Development of a high-density linkage map and mapping of the three-pistil gene (Pis1) in wheat using GBS markers

BACKGROUND: The wheat mutant line three-pistil (TP) exhibits three pistils per floret. As TP normally has two or three seeds in each of the florets on the same spike, there is the possibility of increasing the number of grains per spike. Therefore, TP is a highly valuable mutant for breeding and for...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Zaijun, Chen, Zhenyong, Peng, Zhengsong, Yu, Yan, Liao, Mingli, Wei, Shuhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5537994/
https://www.ncbi.nlm.nih.gov/pubmed/28760136
http://dx.doi.org/10.1186/s12864-017-3960-7
_version_ 1783254284525109248
author Yang, Zaijun
Chen, Zhenyong
Peng, Zhengsong
Yu, Yan
Liao, Mingli
Wei, Shuhong
author_facet Yang, Zaijun
Chen, Zhenyong
Peng, Zhengsong
Yu, Yan
Liao, Mingli
Wei, Shuhong
author_sort Yang, Zaijun
collection PubMed
description BACKGROUND: The wheat mutant line three-pistil (TP) exhibits three pistils per floret. As TP normally has two or three seeds in each of the florets on the same spike, there is the possibility of increasing the number of grains per spike. Therefore, TP is a highly valuable mutant for breeding and for the study of floral development in wheat. To map the three-pistil gene (Pis1), genotyping-by-sequencing single-nucleotide polymorphism (GBS-SNP) data from an F(2) mapping population (CM28 × CM28TP) was used to construct a genetic map that is of significant value. RESULTS: In the present study, a high-density genetic map of wheat containing 2917 GBS-SNP markers was constructed. Twenty-one linkage groups were resolved, with a total length of 2371.40 cM. The individual chromosomes range from 2.64 cM to 454.55 cM with an average marker density of 0.81 cM. The Pis1 gene was mapped using this high-resolution map, and two flanking SNP markers tightly linked to the gene, M70 and M71, were identified. The Pis1 is 3.00 cM from M70 and 1.10 cM from M71. In bread wheat genome, M70 and M71 were found to delimit a physical distance of 3.40 Mb, which encompasses 127 protein-coding genes. To validate the GBS-generated genotypic data and to eliminate missing marker data in the Pis1 region, five Kompetitive Allele-Specific PCR (KASP) assays were designed from corresponding GBS sequences, which harbor SNPs that surround Pis1. Three KASP-SNP markers, KM70, KM71, and KM75, were remapped to the Pis1 gene region. CONCLUSIONS: This work not only lays the foundation for the map-based cloning of Pis1 but can also serve as a valuable tool for studying marker-trait association of important traits and marker-assisted breeding in wheat. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-017-3960-7) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5537994
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-55379942017-08-04 Development of a high-density linkage map and mapping of the three-pistil gene (Pis1) in wheat using GBS markers Yang, Zaijun Chen, Zhenyong Peng, Zhengsong Yu, Yan Liao, Mingli Wei, Shuhong BMC Genomics Research Article BACKGROUND: The wheat mutant line three-pistil (TP) exhibits three pistils per floret. As TP normally has two or three seeds in each of the florets on the same spike, there is the possibility of increasing the number of grains per spike. Therefore, TP is a highly valuable mutant for breeding and for the study of floral development in wheat. To map the three-pistil gene (Pis1), genotyping-by-sequencing single-nucleotide polymorphism (GBS-SNP) data from an F(2) mapping population (CM28 × CM28TP) was used to construct a genetic map that is of significant value. RESULTS: In the present study, a high-density genetic map of wheat containing 2917 GBS-SNP markers was constructed. Twenty-one linkage groups were resolved, with a total length of 2371.40 cM. The individual chromosomes range from 2.64 cM to 454.55 cM with an average marker density of 0.81 cM. The Pis1 gene was mapped using this high-resolution map, and two flanking SNP markers tightly linked to the gene, M70 and M71, were identified. The Pis1 is 3.00 cM from M70 and 1.10 cM from M71. In bread wheat genome, M70 and M71 were found to delimit a physical distance of 3.40 Mb, which encompasses 127 protein-coding genes. To validate the GBS-generated genotypic data and to eliminate missing marker data in the Pis1 region, five Kompetitive Allele-Specific PCR (KASP) assays were designed from corresponding GBS sequences, which harbor SNPs that surround Pis1. Three KASP-SNP markers, KM70, KM71, and KM75, were remapped to the Pis1 gene region. CONCLUSIONS: This work not only lays the foundation for the map-based cloning of Pis1 but can also serve as a valuable tool for studying marker-trait association of important traits and marker-assisted breeding in wheat. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-017-3960-7) contains supplementary material, which is available to authorized users. BioMed Central 2017-07-31 /pmc/articles/PMC5537994/ /pubmed/28760136 http://dx.doi.org/10.1186/s12864-017-3960-7 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Yang, Zaijun
Chen, Zhenyong
Peng, Zhengsong
Yu, Yan
Liao, Mingli
Wei, Shuhong
Development of a high-density linkage map and mapping of the three-pistil gene (Pis1) in wheat using GBS markers
title Development of a high-density linkage map and mapping of the three-pistil gene (Pis1) in wheat using GBS markers
title_full Development of a high-density linkage map and mapping of the three-pistil gene (Pis1) in wheat using GBS markers
title_fullStr Development of a high-density linkage map and mapping of the three-pistil gene (Pis1) in wheat using GBS markers
title_full_unstemmed Development of a high-density linkage map and mapping of the three-pistil gene (Pis1) in wheat using GBS markers
title_short Development of a high-density linkage map and mapping of the three-pistil gene (Pis1) in wheat using GBS markers
title_sort development of a high-density linkage map and mapping of the three-pistil gene (pis1) in wheat using gbs markers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5537994/
https://www.ncbi.nlm.nih.gov/pubmed/28760136
http://dx.doi.org/10.1186/s12864-017-3960-7
work_keys_str_mv AT yangzaijun developmentofahighdensitylinkagemapandmappingofthethreepistilgenepis1inwheatusinggbsmarkers
AT chenzhenyong developmentofahighdensitylinkagemapandmappingofthethreepistilgenepis1inwheatusinggbsmarkers
AT pengzhengsong developmentofahighdensitylinkagemapandmappingofthethreepistilgenepis1inwheatusinggbsmarkers
AT yuyan developmentofahighdensitylinkagemapandmappingofthethreepistilgenepis1inwheatusinggbsmarkers
AT liaomingli developmentofahighdensitylinkagemapandmappingofthethreepistilgenepis1inwheatusinggbsmarkers
AT weishuhong developmentofahighdensitylinkagemapandmappingofthethreepistilgenepis1inwheatusinggbsmarkers