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Isolation and characterization of a Bacillus amyloliquefaciens strain with zearalenone removal ability and its probiotic potential

Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by Fusarium species, which has been shown to be associated with reproductive disorders in livestock, and to a lesser extent with hyperoestrogenic syndromes in humans. The aim of this study was to characterize a Bacillus amyloliquefac...

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Autores principales: Lee, An, Cheng, Kuan-Chen, Liu, Je-Ruei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5538671/
https://www.ncbi.nlm.nih.gov/pubmed/28763483
http://dx.doi.org/10.1371/journal.pone.0182220
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author Lee, An
Cheng, Kuan-Chen
Liu, Je-Ruei
author_facet Lee, An
Cheng, Kuan-Chen
Liu, Je-Ruei
author_sort Lee, An
collection PubMed
description Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by Fusarium species, which has been shown to be associated with reproductive disorders in livestock, and to a lesser extent with hyperoestrogenic syndromes in humans. The aim of this study was to characterize a Bacillus amyloliquefaciens strain with ZEN removal ability. A pure culture of a strain designated LN isolated from moldy corn samples showed a high ZEN removal capability. Based on microscopic observations, biochemical characteristics, and phylogenetic analysis of the 16S rRNA gene sequence, LN was identified as B. amyloliquefaciens. After incubation of B. amyloliquefaciens LN in Luria-Bertani (LB) medium containing 3.5 ppm of ZEN, the ZEN concentration fell below the detection limit within 24 h. In ZEN-contaminated corn meal medium, B. amyloliquefaciens LN decreased ZEN concentration by 92% after 36 h of incubation. In phosphate-buffered saline (PBS) containing 5 ppm of ZEN, B. amyloliquefaciens LN reduced the ZEN concentration from 5 ppm to 3.28 ppm immediately after coming into contact with ZEN, and further reduced the ZEN concentration to 0.36 ppm after 4 h of incubation. The amounts of ZEN adsorbed by the cells of B. amyloliquefaciens LN did not increase with the extension of incubation time, indicating that B. amyloliquefaciens LN not only possessed ZEN adsorption ability, but also exhibited the ability to degrade ZEN. In addition, B. amyloliquefaciens LN was non-hemolytic, non-enterotoxin producing, and displayed probiotic characteristics including acidic tolerance, bile salt tolerance, and anti-pathogenic activities. These findings suggest that B. amyloliquefaciens LN has a potential to be used as a feed additive to reduce the concentrations of ZEN in feedstuffs.
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spelling pubmed-55386712017-08-07 Isolation and characterization of a Bacillus amyloliquefaciens strain with zearalenone removal ability and its probiotic potential Lee, An Cheng, Kuan-Chen Liu, Je-Ruei PLoS One Research Article Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by Fusarium species, which has been shown to be associated with reproductive disorders in livestock, and to a lesser extent with hyperoestrogenic syndromes in humans. The aim of this study was to characterize a Bacillus amyloliquefaciens strain with ZEN removal ability. A pure culture of a strain designated LN isolated from moldy corn samples showed a high ZEN removal capability. Based on microscopic observations, biochemical characteristics, and phylogenetic analysis of the 16S rRNA gene sequence, LN was identified as B. amyloliquefaciens. After incubation of B. amyloliquefaciens LN in Luria-Bertani (LB) medium containing 3.5 ppm of ZEN, the ZEN concentration fell below the detection limit within 24 h. In ZEN-contaminated corn meal medium, B. amyloliquefaciens LN decreased ZEN concentration by 92% after 36 h of incubation. In phosphate-buffered saline (PBS) containing 5 ppm of ZEN, B. amyloliquefaciens LN reduced the ZEN concentration from 5 ppm to 3.28 ppm immediately after coming into contact with ZEN, and further reduced the ZEN concentration to 0.36 ppm after 4 h of incubation. The amounts of ZEN adsorbed by the cells of B. amyloliquefaciens LN did not increase with the extension of incubation time, indicating that B. amyloliquefaciens LN not only possessed ZEN adsorption ability, but also exhibited the ability to degrade ZEN. In addition, B. amyloliquefaciens LN was non-hemolytic, non-enterotoxin producing, and displayed probiotic characteristics including acidic tolerance, bile salt tolerance, and anti-pathogenic activities. These findings suggest that B. amyloliquefaciens LN has a potential to be used as a feed additive to reduce the concentrations of ZEN in feedstuffs. Public Library of Science 2017-08-01 /pmc/articles/PMC5538671/ /pubmed/28763483 http://dx.doi.org/10.1371/journal.pone.0182220 Text en © 2017 Lee et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lee, An
Cheng, Kuan-Chen
Liu, Je-Ruei
Isolation and characterization of a Bacillus amyloliquefaciens strain with zearalenone removal ability and its probiotic potential
title Isolation and characterization of a Bacillus amyloliquefaciens strain with zearalenone removal ability and its probiotic potential
title_full Isolation and characterization of a Bacillus amyloliquefaciens strain with zearalenone removal ability and its probiotic potential
title_fullStr Isolation and characterization of a Bacillus amyloliquefaciens strain with zearalenone removal ability and its probiotic potential
title_full_unstemmed Isolation and characterization of a Bacillus amyloliquefaciens strain with zearalenone removal ability and its probiotic potential
title_short Isolation and characterization of a Bacillus amyloliquefaciens strain with zearalenone removal ability and its probiotic potential
title_sort isolation and characterization of a bacillus amyloliquefaciens strain with zearalenone removal ability and its probiotic potential
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5538671/
https://www.ncbi.nlm.nih.gov/pubmed/28763483
http://dx.doi.org/10.1371/journal.pone.0182220
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