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Effects of irradiance and prey deprivation on growth, cell carbon and photosynthetic activity of the freshwater kleptoplastidic dinoflagellate Nusuttodinium (= Gymnodinium) aeruginosum (Dinophyceae)

The freshwater dinoflagellate Nusuttodinium aeruginosum lacks permanent chloroplasts. Rather it sequesters chloroplasts as well as other cell organelles, like mitochondria and nuclei, from ingested cryptophyte prey. In the present study, growth rates, cell production and photosynthesis were measured...

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Autores principales: Drumm, Kirstine, Liebst-Olsen, Mette, Daugbjerg, Niels, Moestrup, Øjvind, Hansen, Per Juel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5538715/
https://www.ncbi.nlm.nih.gov/pubmed/28763480
http://dx.doi.org/10.1371/journal.pone.0181751
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author Drumm, Kirstine
Liebst-Olsen, Mette
Daugbjerg, Niels
Moestrup, Øjvind
Hansen, Per Juel
author_facet Drumm, Kirstine
Liebst-Olsen, Mette
Daugbjerg, Niels
Moestrup, Øjvind
Hansen, Per Juel
author_sort Drumm, Kirstine
collection PubMed
description The freshwater dinoflagellate Nusuttodinium aeruginosum lacks permanent chloroplasts. Rather it sequesters chloroplasts as well as other cell organelles, like mitochondria and nuclei, from ingested cryptophyte prey. In the present study, growth rates, cell production and photosynthesis were measured at seven irradiances, ranging from 10 to 140 μmol photons m(-2)s(-1), when fed the cryptophyte Chroomonas sp. Growth rates were positively influenced by irradiance and increased from 0.025 d(-1) at 10 μmol photons m(-2)s(-1) to maximum growth rates of ~0.3 d(-1) at irradiances ≥ 40 μmol photons m(-2)s(-1). Similarly, photosynthesis ranged from 1.84 to 36.9 pg C cell(-1) h(-1) at 10 and 140 μmol photons m(-2)s(-1), respectively. The highest rates of photosynthesis in N. aeruginosum only corresponded to ~25% of its own cell carbon content and estimated biomass production. The measured rates of photosynthesis could not explain the observed growth rates at high irradiances. Cultures of N. aeruginosum subjected to prey starvation were able to survive for at least 27 days in the light. The sequestered chloroplasts maintained their photosynthetic activity during the entire period of starvation, during which the population underwent 4 cell divisions. This indicates that N. aeruginosum has some control of the chloroplasts, which may be able to replicate. In conclusion, N. aeruginosum seems to be in an early stage of chloroplast acquisition with some control of its ingested chloroplasts.
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spelling pubmed-55387152017-08-07 Effects of irradiance and prey deprivation on growth, cell carbon and photosynthetic activity of the freshwater kleptoplastidic dinoflagellate Nusuttodinium (= Gymnodinium) aeruginosum (Dinophyceae) Drumm, Kirstine Liebst-Olsen, Mette Daugbjerg, Niels Moestrup, Øjvind Hansen, Per Juel PLoS One Research Article The freshwater dinoflagellate Nusuttodinium aeruginosum lacks permanent chloroplasts. Rather it sequesters chloroplasts as well as other cell organelles, like mitochondria and nuclei, from ingested cryptophyte prey. In the present study, growth rates, cell production and photosynthesis were measured at seven irradiances, ranging from 10 to 140 μmol photons m(-2)s(-1), when fed the cryptophyte Chroomonas sp. Growth rates were positively influenced by irradiance and increased from 0.025 d(-1) at 10 μmol photons m(-2)s(-1) to maximum growth rates of ~0.3 d(-1) at irradiances ≥ 40 μmol photons m(-2)s(-1). Similarly, photosynthesis ranged from 1.84 to 36.9 pg C cell(-1) h(-1) at 10 and 140 μmol photons m(-2)s(-1), respectively. The highest rates of photosynthesis in N. aeruginosum only corresponded to ~25% of its own cell carbon content and estimated biomass production. The measured rates of photosynthesis could not explain the observed growth rates at high irradiances. Cultures of N. aeruginosum subjected to prey starvation were able to survive for at least 27 days in the light. The sequestered chloroplasts maintained their photosynthetic activity during the entire period of starvation, during which the population underwent 4 cell divisions. This indicates that N. aeruginosum has some control of the chloroplasts, which may be able to replicate. In conclusion, N. aeruginosum seems to be in an early stage of chloroplast acquisition with some control of its ingested chloroplasts. Public Library of Science 2017-08-01 /pmc/articles/PMC5538715/ /pubmed/28763480 http://dx.doi.org/10.1371/journal.pone.0181751 Text en © 2017 Drumm et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Drumm, Kirstine
Liebst-Olsen, Mette
Daugbjerg, Niels
Moestrup, Øjvind
Hansen, Per Juel
Effects of irradiance and prey deprivation on growth, cell carbon and photosynthetic activity of the freshwater kleptoplastidic dinoflagellate Nusuttodinium (= Gymnodinium) aeruginosum (Dinophyceae)
title Effects of irradiance and prey deprivation on growth, cell carbon and photosynthetic activity of the freshwater kleptoplastidic dinoflagellate Nusuttodinium (= Gymnodinium) aeruginosum (Dinophyceae)
title_full Effects of irradiance and prey deprivation on growth, cell carbon and photosynthetic activity of the freshwater kleptoplastidic dinoflagellate Nusuttodinium (= Gymnodinium) aeruginosum (Dinophyceae)
title_fullStr Effects of irradiance and prey deprivation on growth, cell carbon and photosynthetic activity of the freshwater kleptoplastidic dinoflagellate Nusuttodinium (= Gymnodinium) aeruginosum (Dinophyceae)
title_full_unstemmed Effects of irradiance and prey deprivation on growth, cell carbon and photosynthetic activity of the freshwater kleptoplastidic dinoflagellate Nusuttodinium (= Gymnodinium) aeruginosum (Dinophyceae)
title_short Effects of irradiance and prey deprivation on growth, cell carbon and photosynthetic activity of the freshwater kleptoplastidic dinoflagellate Nusuttodinium (= Gymnodinium) aeruginosum (Dinophyceae)
title_sort effects of irradiance and prey deprivation on growth, cell carbon and photosynthetic activity of the freshwater kleptoplastidic dinoflagellate nusuttodinium (= gymnodinium) aeruginosum (dinophyceae)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5538715/
https://www.ncbi.nlm.nih.gov/pubmed/28763480
http://dx.doi.org/10.1371/journal.pone.0181751
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