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CVD graphene as an electrochemical sensing platform for simultaneous detection of biomolecules
The development of electrochemical biosensors for the simultaneous detection of ascorbic acid (AA), dopamine (DA), uric acid (UA), tryptophan (Trp), and nitrite ([Formula: see text] ) in human serum is reported in this work. Free-standing graphene nanosheets were fabricated on Ta wire using the chem...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539141/ https://www.ncbi.nlm.nih.gov/pubmed/28765640 http://dx.doi.org/10.1038/s41598-017-07646-2 |
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author | Wang, Xiaodan Gao, Delan Li, Mingji Li, Hongji Li, Cuiping Wu, Xiaoguo Yang, Baohe |
author_facet | Wang, Xiaodan Gao, Delan Li, Mingji Li, Hongji Li, Cuiping Wu, Xiaoguo Yang, Baohe |
author_sort | Wang, Xiaodan |
collection | PubMed |
description | The development of electrochemical biosensors for the simultaneous detection of ascorbic acid (AA), dopamine (DA), uric acid (UA), tryptophan (Trp), and nitrite ([Formula: see text] ) in human serum is reported in this work. Free-standing graphene nanosheets were fabricated on Ta wire using the chemical vapor deposition (CVD) method. CVD graphene, which here served as a sensing platform, provided a highly sensitive and selective option, with detection limits of AA, DA, UA, Trp, and [Formula: see text] of 1.58, 0.06, 0.09, 0.10, and 6.45 μM (S/N = 3), respectively. The high selectivity of the electrode is here explained by a relationship between the bandgap energy of analyte and the Fermi level of graphene. The high sensitivity in the oxidation current was determined by analyzing the influence of the high surface area and chemical structure of free-standing graphene nanosheets on analyte adsorption capacity. This finding strongly indicates that the CVD graphene electrode can be used as a biosensor to detect five analytes in human serum. |
format | Online Article Text |
id | pubmed-5539141 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55391412017-08-07 CVD graphene as an electrochemical sensing platform for simultaneous detection of biomolecules Wang, Xiaodan Gao, Delan Li, Mingji Li, Hongji Li, Cuiping Wu, Xiaoguo Yang, Baohe Sci Rep Article The development of electrochemical biosensors for the simultaneous detection of ascorbic acid (AA), dopamine (DA), uric acid (UA), tryptophan (Trp), and nitrite ([Formula: see text] ) in human serum is reported in this work. Free-standing graphene nanosheets were fabricated on Ta wire using the chemical vapor deposition (CVD) method. CVD graphene, which here served as a sensing platform, provided a highly sensitive and selective option, with detection limits of AA, DA, UA, Trp, and [Formula: see text] of 1.58, 0.06, 0.09, 0.10, and 6.45 μM (S/N = 3), respectively. The high selectivity of the electrode is here explained by a relationship between the bandgap energy of analyte and the Fermi level of graphene. The high sensitivity in the oxidation current was determined by analyzing the influence of the high surface area and chemical structure of free-standing graphene nanosheets on analyte adsorption capacity. This finding strongly indicates that the CVD graphene electrode can be used as a biosensor to detect five analytes in human serum. Nature Publishing Group UK 2017-08-01 /pmc/articles/PMC5539141/ /pubmed/28765640 http://dx.doi.org/10.1038/s41598-017-07646-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Wang, Xiaodan Gao, Delan Li, Mingji Li, Hongji Li, Cuiping Wu, Xiaoguo Yang, Baohe CVD graphene as an electrochemical sensing platform for simultaneous detection of biomolecules |
title | CVD graphene as an electrochemical sensing platform for simultaneous detection of biomolecules |
title_full | CVD graphene as an electrochemical sensing platform for simultaneous detection of biomolecules |
title_fullStr | CVD graphene as an electrochemical sensing platform for simultaneous detection of biomolecules |
title_full_unstemmed | CVD graphene as an electrochemical sensing platform for simultaneous detection of biomolecules |
title_short | CVD graphene as an electrochemical sensing platform for simultaneous detection of biomolecules |
title_sort | cvd graphene as an electrochemical sensing platform for simultaneous detection of biomolecules |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539141/ https://www.ncbi.nlm.nih.gov/pubmed/28765640 http://dx.doi.org/10.1038/s41598-017-07646-2 |
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