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Bacteriophages as potential new mammalian pathogens
Increased intestinal permeability and translocation of gut bacteria trigger various polyaetiological diseases associated with chronic inflammation and underlie a variety of poorly treatable pathologies. Previous studies have established a primary role of the microbiota composition and intestinal per...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539208/ https://www.ncbi.nlm.nih.gov/pubmed/28765534 http://dx.doi.org/10.1038/s41598-017-07278-6 |
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author | Tetz, George V. Ruggles, Kelly V. Zhou, Hua Heguy, Adriana Tsirigos, Aristotelis Tetz, Victor |
author_facet | Tetz, George V. Ruggles, Kelly V. Zhou, Hua Heguy, Adriana Tsirigos, Aristotelis Tetz, Victor |
author_sort | Tetz, George V. |
collection | PubMed |
description | Increased intestinal permeability and translocation of gut bacteria trigger various polyaetiological diseases associated with chronic inflammation and underlie a variety of poorly treatable pathologies. Previous studies have established a primary role of the microbiota composition and intestinal permeability in such pathologies. Using a rat model, we examined the effects of exposure to a bacteriophage cocktail on intestinal permeability and relative abundance of taxonomic units in the gut bacterial community. There was an increase in markers of impaired gut permeability, such as the lactulose/mannitol ratio, plasma endotoxin concentrations, and serum levels of inflammation-related cytokines, following the bacteriophage challenge. We observed significant differences in the alpha diversity of faecal bacterial species and found that richness and diversity index values increased following the bacteriophage challenge. There was a reduction in the abundance of Blautia, Catenibacterium, Lactobacillus, and Faecalibacterium species and an increase in Butyrivibrio, Oscillospira and Ruminococcus after bacteriophage administration. These findings provide novel insights into the role of bacteriophages as potentially pathogenic for mammals and their possible implication in the development of diseases associated with increased intestinal permeability. |
format | Online Article Text |
id | pubmed-5539208 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55392082017-08-07 Bacteriophages as potential new mammalian pathogens Tetz, George V. Ruggles, Kelly V. Zhou, Hua Heguy, Adriana Tsirigos, Aristotelis Tetz, Victor Sci Rep Article Increased intestinal permeability and translocation of gut bacteria trigger various polyaetiological diseases associated with chronic inflammation and underlie a variety of poorly treatable pathologies. Previous studies have established a primary role of the microbiota composition and intestinal permeability in such pathologies. Using a rat model, we examined the effects of exposure to a bacteriophage cocktail on intestinal permeability and relative abundance of taxonomic units in the gut bacterial community. There was an increase in markers of impaired gut permeability, such as the lactulose/mannitol ratio, plasma endotoxin concentrations, and serum levels of inflammation-related cytokines, following the bacteriophage challenge. We observed significant differences in the alpha diversity of faecal bacterial species and found that richness and diversity index values increased following the bacteriophage challenge. There was a reduction in the abundance of Blautia, Catenibacterium, Lactobacillus, and Faecalibacterium species and an increase in Butyrivibrio, Oscillospira and Ruminococcus after bacteriophage administration. These findings provide novel insights into the role of bacteriophages as potentially pathogenic for mammals and their possible implication in the development of diseases associated with increased intestinal permeability. Nature Publishing Group UK 2017-08-01 /pmc/articles/PMC5539208/ /pubmed/28765534 http://dx.doi.org/10.1038/s41598-017-07278-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Tetz, George V. Ruggles, Kelly V. Zhou, Hua Heguy, Adriana Tsirigos, Aristotelis Tetz, Victor Bacteriophages as potential new mammalian pathogens |
title | Bacteriophages as potential new mammalian pathogens |
title_full | Bacteriophages as potential new mammalian pathogens |
title_fullStr | Bacteriophages as potential new mammalian pathogens |
title_full_unstemmed | Bacteriophages as potential new mammalian pathogens |
title_short | Bacteriophages as potential new mammalian pathogens |
title_sort | bacteriophages as potential new mammalian pathogens |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539208/ https://www.ncbi.nlm.nih.gov/pubmed/28765534 http://dx.doi.org/10.1038/s41598-017-07278-6 |
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