Cargando…

The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction

Since transplantation of meniscal allograft or artificial menisci is limited by graft sources and a series of adverse events, substitution for meniscus reconstruction still needs to be explored. Natural biomaterials, which can provide a unique 3-D microenvironment, remain a promising alternative for...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Chenxi, Hu, Xiaoqing, Meng, Qingyang, Zhang, Xin, Zhu, Jingxian, Dai, Linghui, Cheng, Jin, Zhong, Mingjin, Shi, Weili, Ren, Bo, Zhang, Jiying, Fu, Xin, Duan, Xiaoning, Ao, Yingfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539314/
https://www.ncbi.nlm.nih.gov/pubmed/28765605
http://dx.doi.org/10.1038/s41598-017-07166-z
_version_ 1783254467136716800
author Li, Chenxi
Hu, Xiaoqing
Meng, Qingyang
Zhang, Xin
Zhu, Jingxian
Dai, Linghui
Cheng, Jin
Zhong, Mingjin
Shi, Weili
Ren, Bo
Zhang, Jiying
Fu, Xin
Duan, Xiaoning
Ao, Yingfang
author_facet Li, Chenxi
Hu, Xiaoqing
Meng, Qingyang
Zhang, Xin
Zhu, Jingxian
Dai, Linghui
Cheng, Jin
Zhong, Mingjin
Shi, Weili
Ren, Bo
Zhang, Jiying
Fu, Xin
Duan, Xiaoning
Ao, Yingfang
author_sort Li, Chenxi
collection PubMed
description Since transplantation of meniscal allograft or artificial menisci is limited by graft sources and a series of adverse events, substitution for meniscus reconstruction still needs to be explored. Natural biomaterials, which can provide a unique 3-D microenvironment, remain a promising alternative for tissue engineering. Among them, autograft is a preferred option for its safety and excellent biocompatibility. In this study, we utilized semitendinosus tendon autograft in meniscus reconstruction to investigate its fibrochondrogenic metaplasticity potential and chondroprotective effect. Tendon-derived stem cells (TDSCs) and synovial-derived mesenchymal stem cells (SMSCs), two most important stem cell sources in our strategy, exhibited excellent viability, distribution, proliferation and fibrochondrogenic differentiation ability in decellularized semitendinosus tendon (DST) scaffolds in vitro. Histologic evaluation of the tendon grafts in vivo suggested endogenous stem cells differentiated into fibrochondrocytes, synthesized proteoglycan, type II collagen and radial type I collagen at 12 weeks and 24 weeks post-surgery. As for elastic modulus and hardness of the grafts, there were no significant differences between native meniscus and regenerated meniscus at 24 weeks. The protection of condylar cartilage from degeneration was significantly better in the reconstruction group comparing to control group. Overall, semitendinosus tendon autograft seems to be a promising substitution in meniscus reconstruction.
format Online
Article
Text
id pubmed-5539314
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-55393142017-08-07 The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction Li, Chenxi Hu, Xiaoqing Meng, Qingyang Zhang, Xin Zhu, Jingxian Dai, Linghui Cheng, Jin Zhong, Mingjin Shi, Weili Ren, Bo Zhang, Jiying Fu, Xin Duan, Xiaoning Ao, Yingfang Sci Rep Article Since transplantation of meniscal allograft or artificial menisci is limited by graft sources and a series of adverse events, substitution for meniscus reconstruction still needs to be explored. Natural biomaterials, which can provide a unique 3-D microenvironment, remain a promising alternative for tissue engineering. Among them, autograft is a preferred option for its safety and excellent biocompatibility. In this study, we utilized semitendinosus tendon autograft in meniscus reconstruction to investigate its fibrochondrogenic metaplasticity potential and chondroprotective effect. Tendon-derived stem cells (TDSCs) and synovial-derived mesenchymal stem cells (SMSCs), two most important stem cell sources in our strategy, exhibited excellent viability, distribution, proliferation and fibrochondrogenic differentiation ability in decellularized semitendinosus tendon (DST) scaffolds in vitro. Histologic evaluation of the tendon grafts in vivo suggested endogenous stem cells differentiated into fibrochondrocytes, synthesized proteoglycan, type II collagen and radial type I collagen at 12 weeks and 24 weeks post-surgery. As for elastic modulus and hardness of the grafts, there were no significant differences between native meniscus and regenerated meniscus at 24 weeks. The protection of condylar cartilage from degeneration was significantly better in the reconstruction group comparing to control group. Overall, semitendinosus tendon autograft seems to be a promising substitution in meniscus reconstruction. Nature Publishing Group UK 2017-08-01 /pmc/articles/PMC5539314/ /pubmed/28765605 http://dx.doi.org/10.1038/s41598-017-07166-z Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Chenxi
Hu, Xiaoqing
Meng, Qingyang
Zhang, Xin
Zhu, Jingxian
Dai, Linghui
Cheng, Jin
Zhong, Mingjin
Shi, Weili
Ren, Bo
Zhang, Jiying
Fu, Xin
Duan, Xiaoning
Ao, Yingfang
The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction
title The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction
title_full The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction
title_fullStr The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction
title_full_unstemmed The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction
title_short The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction
title_sort potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539314/
https://www.ncbi.nlm.nih.gov/pubmed/28765605
http://dx.doi.org/10.1038/s41598-017-07166-z
work_keys_str_mv AT lichenxi thepotentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT huxiaoqing thepotentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT mengqingyang thepotentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT zhangxin thepotentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT zhujingxian thepotentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT dailinghui thepotentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT chengjin thepotentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT zhongmingjin thepotentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT shiweili thepotentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT renbo thepotentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT zhangjiying thepotentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT fuxin thepotentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT duanxiaoning thepotentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT aoyingfang thepotentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT lichenxi potentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT huxiaoqing potentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT mengqingyang potentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT zhangxin potentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT zhujingxian potentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT dailinghui potentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT chengjin potentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT zhongmingjin potentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT shiweili potentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT renbo potentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT zhangjiying potentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT fuxin potentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT duanxiaoning potentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction
AT aoyingfang potentialofusingsemitendinosustendonasautograftinrabbitmeniscusreconstruction