Detection of Guanine and Adenine Using an Aminated Reduced Graphene Oxide Functional Membrane-Modified Glassy Carbon Electrode

A new electrochemical sensor based on a Nafion, aminated reduced graphene oxide and chitosan functional membrane-modified glassy carbon electrode was proposed for the simultaneous detection of adenine and guanine. Fourier transform-infrared spectrometry (FTIR), transmission electron microscopy (TEM)...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Di, Yang, Xiao-Lu, Xiao, Bao-Lin, Geng, Fang-Yong, Hong, Jun, Sheibani, Nader, Moosavi-Movahedi, Ali Akbar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539557/
https://www.ncbi.nlm.nih.gov/pubmed/28718793
http://dx.doi.org/10.3390/s17071652
_version_ 1783254501914836992
author Li, Di
Yang, Xiao-Lu
Xiao, Bao-Lin
Geng, Fang-Yong
Hong, Jun
Sheibani, Nader
Moosavi-Movahedi, Ali Akbar
author_facet Li, Di
Yang, Xiao-Lu
Xiao, Bao-Lin
Geng, Fang-Yong
Hong, Jun
Sheibani, Nader
Moosavi-Movahedi, Ali Akbar
author_sort Li, Di
collection PubMed
description A new electrochemical sensor based on a Nafion, aminated reduced graphene oxide and chitosan functional membrane-modified glassy carbon electrode was proposed for the simultaneous detection of adenine and guanine. Fourier transform-infrared spectrometry (FTIR), transmission electron microscopy (TEM), and electrochemical methods were utilized for the additional characterization of the membrane materials. The prepared electrode was utilized for the detection of guanine (G) and adenine (A). The anodic peak currents to G and A were linear in the concentrations ranging from 0.1 to 120 μM and 0.2 to 110 μM, respectively. The detection limits were found to be 0.1 μM and 0.2 μM, respectively. Moreover, the modified electrode could also be used to determine G and A in calf thymus DNA.
format Online
Article
Text
id pubmed-5539557
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-55395572017-08-11 Detection of Guanine and Adenine Using an Aminated Reduced Graphene Oxide Functional Membrane-Modified Glassy Carbon Electrode Li, Di Yang, Xiao-Lu Xiao, Bao-Lin Geng, Fang-Yong Hong, Jun Sheibani, Nader Moosavi-Movahedi, Ali Akbar Sensors (Basel) Article A new electrochemical sensor based on a Nafion, aminated reduced graphene oxide and chitosan functional membrane-modified glassy carbon electrode was proposed for the simultaneous detection of adenine and guanine. Fourier transform-infrared spectrometry (FTIR), transmission electron microscopy (TEM), and electrochemical methods were utilized for the additional characterization of the membrane materials. The prepared electrode was utilized for the detection of guanine (G) and adenine (A). The anodic peak currents to G and A were linear in the concentrations ranging from 0.1 to 120 μM and 0.2 to 110 μM, respectively. The detection limits were found to be 0.1 μM and 0.2 μM, respectively. Moreover, the modified electrode could also be used to determine G and A in calf thymus DNA. MDPI 2017-07-18 /pmc/articles/PMC5539557/ /pubmed/28718793 http://dx.doi.org/10.3390/s17071652 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Di
Yang, Xiao-Lu
Xiao, Bao-Lin
Geng, Fang-Yong
Hong, Jun
Sheibani, Nader
Moosavi-Movahedi, Ali Akbar
Detection of Guanine and Adenine Using an Aminated Reduced Graphene Oxide Functional Membrane-Modified Glassy Carbon Electrode
title Detection of Guanine and Adenine Using an Aminated Reduced Graphene Oxide Functional Membrane-Modified Glassy Carbon Electrode
title_full Detection of Guanine and Adenine Using an Aminated Reduced Graphene Oxide Functional Membrane-Modified Glassy Carbon Electrode
title_fullStr Detection of Guanine and Adenine Using an Aminated Reduced Graphene Oxide Functional Membrane-Modified Glassy Carbon Electrode
title_full_unstemmed Detection of Guanine and Adenine Using an Aminated Reduced Graphene Oxide Functional Membrane-Modified Glassy Carbon Electrode
title_short Detection of Guanine and Adenine Using an Aminated Reduced Graphene Oxide Functional Membrane-Modified Glassy Carbon Electrode
title_sort detection of guanine and adenine using an aminated reduced graphene oxide functional membrane-modified glassy carbon electrode
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539557/
https://www.ncbi.nlm.nih.gov/pubmed/28718793
http://dx.doi.org/10.3390/s17071652
work_keys_str_mv AT lidi detectionofguanineandadenineusinganaminatedreducedgrapheneoxidefunctionalmembranemodifiedglassycarbonelectrode
AT yangxiaolu detectionofguanineandadenineusinganaminatedreducedgrapheneoxidefunctionalmembranemodifiedglassycarbonelectrode
AT xiaobaolin detectionofguanineandadenineusinganaminatedreducedgrapheneoxidefunctionalmembranemodifiedglassycarbonelectrode
AT gengfangyong detectionofguanineandadenineusinganaminatedreducedgrapheneoxidefunctionalmembranemodifiedglassycarbonelectrode
AT hongjun detectionofguanineandadenineusinganaminatedreducedgrapheneoxidefunctionalmembranemodifiedglassycarbonelectrode
AT sheibaninader detectionofguanineandadenineusinganaminatedreducedgrapheneoxidefunctionalmembranemodifiedglassycarbonelectrode
AT moosavimovahedialiakbar detectionofguanineandadenineusinganaminatedreducedgrapheneoxidefunctionalmembranemodifiedglassycarbonelectrode

Ejemplares similares