Fabrication of Flexible Arrayed Lactate Biosensor Based on Immobilizing LDH-NAD(+) on NiO Film Modified by GO and MBs

We proposed the flexible arrayed lactate biosensor based on immobilizing l-lactate dehydrogenase (LDH) and nicotinamide adenine dinucleotide ([Formula: see text]) on nickel oxide (NiO) film, and which the average sensitivity could be enhanced by using graphene oxide (GO) and magnetic beads (MBs). By...

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Detalles Bibliográficos
Autores principales: Chou, Jung-Chuan, Yan, Siao-Jie, Liao, Yi-Hung, Lai, Chih-Hsien, Wu, You-Xiang, Wu, Cian-Yi, Chen, Hsiang-Yi, Huang, Hong-Yu, Wu, Tong-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539599/
https://www.ncbi.nlm.nih.gov/pubmed/28704960
http://dx.doi.org/10.3390/s17071618
Descripción
Sumario:We proposed the flexible arrayed lactate biosensor based on immobilizing l-lactate dehydrogenase (LDH) and nicotinamide adenine dinucleotide ([Formula: see text]) on nickel oxide (NiO) film, and which the average sensitivity could be enhanced by using graphene oxide (GO) and magnetic beads (MBs). By using GO and MBs, it exhibits excellent sensitivity (45.397 mV/mM) with a linearity of 0.992 in a range of 0.2 mM to 3 mM. According to the results of electrochemical impedance spectroscopy (EIS), the electron transfer resistance of LDH- [Formula: see text]-MBs/GPTS/GO/NiO film was smaller than those of LDH-NAD(+)/GPTS/GO/NiO film and LDH- [Formula: see text] /GPTS/NiO film, and it presented the outstanding electron transfer ability. After that, the limit of detection, anti-interference effect and bending test were also investigated.

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