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A Label-Free Fluorescent Array Sensor Utilizing Liposome Encapsulating Calcein for Discriminating Target Proteins by Principal Component Analysis

A new fluorescent arrayed biosensor has been developed to discriminate species and concentrations of target proteins by using plural different phospholipid liposome species encapsulating fluorescent molecules, utilizing differences in permeation of the fluorescent molecules through the membrane to m...

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Detalles Bibliográficos
Autores principales: Imamura, Ryota, Murata, Naoki, Shimanouchi, Toshinori, Yamashita, Kaoru, Fukuzawa, Masayuki, Noda, Minoru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539792/
https://www.ncbi.nlm.nih.gov/pubmed/28714873
http://dx.doi.org/10.3390/s17071630
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author Imamura, Ryota
Murata, Naoki
Shimanouchi, Toshinori
Yamashita, Kaoru
Fukuzawa, Masayuki
Noda, Minoru
author_facet Imamura, Ryota
Murata, Naoki
Shimanouchi, Toshinori
Yamashita, Kaoru
Fukuzawa, Masayuki
Noda, Minoru
author_sort Imamura, Ryota
collection PubMed
description A new fluorescent arrayed biosensor has been developed to discriminate species and concentrations of target proteins by using plural different phospholipid liposome species encapsulating fluorescent molecules, utilizing differences in permeation of the fluorescent molecules through the membrane to modulate liposome-target protein interactions. This approach proposes a basically new label-free fluorescent sensor, compared with the common technique of developed fluorescent array sensors with labeling. We have confirmed a high output intensity of fluorescence emission related to characteristics of the fluorescent molecules dependent on their concentrations when they leak from inside the liposomes through the perturbed lipid membrane. After taking an array image of the fluorescence emission from the sensor using a CMOS imager, the output intensities of the fluorescence were analyzed by a principal component analysis (PCA) statistical method. It is found from PCA plots that different protein species with several concentrations were successfully discriminated by using the different lipid membranes with high cumulative contribution ratio. We also confirmed that the accuracy of the discrimination by the array sensor with a single shot is higher than that of a single sensor with multiple shots.
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spelling pubmed-55397922017-08-11 A Label-Free Fluorescent Array Sensor Utilizing Liposome Encapsulating Calcein for Discriminating Target Proteins by Principal Component Analysis Imamura, Ryota Murata, Naoki Shimanouchi, Toshinori Yamashita, Kaoru Fukuzawa, Masayuki Noda, Minoru Sensors (Basel) Article A new fluorescent arrayed biosensor has been developed to discriminate species and concentrations of target proteins by using plural different phospholipid liposome species encapsulating fluorescent molecules, utilizing differences in permeation of the fluorescent molecules through the membrane to modulate liposome-target protein interactions. This approach proposes a basically new label-free fluorescent sensor, compared with the common technique of developed fluorescent array sensors with labeling. We have confirmed a high output intensity of fluorescence emission related to characteristics of the fluorescent molecules dependent on their concentrations when they leak from inside the liposomes through the perturbed lipid membrane. After taking an array image of the fluorescence emission from the sensor using a CMOS imager, the output intensities of the fluorescence were analyzed by a principal component analysis (PCA) statistical method. It is found from PCA plots that different protein species with several concentrations were successfully discriminated by using the different lipid membranes with high cumulative contribution ratio. We also confirmed that the accuracy of the discrimination by the array sensor with a single shot is higher than that of a single sensor with multiple shots. MDPI 2017-07-15 /pmc/articles/PMC5539792/ /pubmed/28714873 http://dx.doi.org/10.3390/s17071630 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Imamura, Ryota
Murata, Naoki
Shimanouchi, Toshinori
Yamashita, Kaoru
Fukuzawa, Masayuki
Noda, Minoru
A Label-Free Fluorescent Array Sensor Utilizing Liposome Encapsulating Calcein for Discriminating Target Proteins by Principal Component Analysis
title A Label-Free Fluorescent Array Sensor Utilizing Liposome Encapsulating Calcein for Discriminating Target Proteins by Principal Component Analysis
title_full A Label-Free Fluorescent Array Sensor Utilizing Liposome Encapsulating Calcein for Discriminating Target Proteins by Principal Component Analysis
title_fullStr A Label-Free Fluorescent Array Sensor Utilizing Liposome Encapsulating Calcein for Discriminating Target Proteins by Principal Component Analysis
title_full_unstemmed A Label-Free Fluorescent Array Sensor Utilizing Liposome Encapsulating Calcein for Discriminating Target Proteins by Principal Component Analysis
title_short A Label-Free Fluorescent Array Sensor Utilizing Liposome Encapsulating Calcein for Discriminating Target Proteins by Principal Component Analysis
title_sort label-free fluorescent array sensor utilizing liposome encapsulating calcein for discriminating target proteins by principal component analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539792/
https://www.ncbi.nlm.nih.gov/pubmed/28714873
http://dx.doi.org/10.3390/s17071630
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