Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe

A series of luminescent Ir(iii) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The Ir(iii) complex 9, [Ir(phq)(2)(phen)]PF(6) (where phq = 2-phenylquinoline; phen = 1,10-phenanthroline), exhibited high luminescence in the presence of G...

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Autores principales: Leung, Ka-Ho, He, Hong-Zhang, He, Bingyong, Zhong, Hai-Jing, Lin, Sheng, Wang, Yi-Tao, Ma, Dik-Lung, Leung, Chung-Hang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2015
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539802/
https://www.ncbi.nlm.nih.gov/pubmed/28808523
http://dx.doi.org/10.1039/c4sc03319a
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author Leung, Ka-Ho
He, Hong-Zhang
He, Bingyong
Zhong, Hai-Jing
Lin, Sheng
Wang, Yi-Tao
Ma, Dik-Lung
Leung, Chung-Hang
author_facet Leung, Ka-Ho
He, Hong-Zhang
He, Bingyong
Zhong, Hai-Jing
Lin, Sheng
Wang, Yi-Tao
Ma, Dik-Lung
Leung, Chung-Hang
author_sort Leung, Ka-Ho
collection PubMed
description A series of luminescent Ir(iii) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The Ir(iii) complex 9, [Ir(phq)(2)(phen)]PF(6) (where phq = 2-phenylquinoline; phen = 1,10-phenanthroline), exhibited high luminescence in the presence of G-quadruplex DNA compared to dsDNA and ssDNA, and was employed to construct a label-free G-quadruplex-based assay for hepatitis C virus NS3 helicase activity in aqueous solution. Moreover, the application of the assay for screening potential helicase inhibitors was demonstrated. To our knowledge, this is the first G-quadruplex-based assay for helicase activity.
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spelling pubmed-55398022017-08-14 Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe Leung, Ka-Ho He, Hong-Zhang He, Bingyong Zhong, Hai-Jing Lin, Sheng Wang, Yi-Tao Ma, Dik-Lung Leung, Chung-Hang Chem Sci Chemistry A series of luminescent Ir(iii) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The Ir(iii) complex 9, [Ir(phq)(2)(phen)]PF(6) (where phq = 2-phenylquinoline; phen = 1,10-phenanthroline), exhibited high luminescence in the presence of G-quadruplex DNA compared to dsDNA and ssDNA, and was employed to construct a label-free G-quadruplex-based assay for hepatitis C virus NS3 helicase activity in aqueous solution. Moreover, the application of the assay for screening potential helicase inhibitors was demonstrated. To our knowledge, this is the first G-quadruplex-based assay for helicase activity. Royal Society of Chemistry 2015-04-01 2014-11-25 /pmc/articles/PMC5539802/ /pubmed/28808523 http://dx.doi.org/10.1039/c4sc03319a Text en This journal is © The Royal Society of Chemistry 2014 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Leung, Ka-Ho
He, Hong-Zhang
He, Bingyong
Zhong, Hai-Jing
Lin, Sheng
Wang, Yi-Tao
Ma, Dik-Lung
Leung, Chung-Hang
Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe
title Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe
title_full Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe
title_fullStr Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe
title_full_unstemmed Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe
title_short Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe
title_sort label-free luminescence switch-on detection of hepatitis c virus ns3 helicase activity using a g-quadruplex-selective probe
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539802/
https://www.ncbi.nlm.nih.gov/pubmed/28808523
http://dx.doi.org/10.1039/c4sc03319a
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