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Testis specific histone 2B is associated with sperm chromatin dynamics and bull fertility-a pilot study
BACKGROUND: Bull fertility is the degree of sperm’s ability to fertilize and activate the egg and support embryo development, and this is critical for herd reproductive performance. We used the bull as a unique model organism for the study of male fertility because cattle genetics and physiology is...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539985/ https://www.ncbi.nlm.nih.gov/pubmed/28764714 http://dx.doi.org/10.1186/s12958-017-0274-1 |
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author | Kutchy, Naseer A. Velho, Ana Menezes, Erika S. B. Jacobsen, Marie Thibaudeau, Giselle Wills, Robert W. Moura, Arlindo Kaya, Abdullah Perkins, Andy Memili, Erdogan |
author_facet | Kutchy, Naseer A. Velho, Ana Menezes, Erika S. B. Jacobsen, Marie Thibaudeau, Giselle Wills, Robert W. Moura, Arlindo Kaya, Abdullah Perkins, Andy Memili, Erdogan |
author_sort | Kutchy, Naseer A. |
collection | PubMed |
description | BACKGROUND: Bull fertility is the degree of sperm’s ability to fertilize and activate the egg and support embryo development, and this is critical for herd reproductive performance. We used the bull as a unique model organism for the study of male fertility because cattle genetics and physiology is similar to those of other mammals including humans. Moreover, reliable fertility data along with well-established in vitro systems are available for bovine. The objective of this original study was to ascertain evolutionary diversification and expression dynamics of Testis Specific Histone 2B (TH2B) in sperm from Holstein bulls with different fertility scores. METHODS: The intensity of TH2B was determined by using flow cytometry in sperm from 13 high and 13 low fertility bulls. Expression levels of TH2B were measured using immunofluorescence and Western blotting in sperm from five high and five low fertility bulls. Sequence identity, evolutionary distance and interactome of TH2B were evaluated by dotmatcher, STRING and Cytoscape. Data were analyzed using linear mixed effects model and regression plots were drawn. RESULTS: The intensity of TH2B as measured by flow cytometry was significantly affected by an interaction between fertility group and fertility score (P = 0.0182). The intensity of TH2B in sperm from the high fertility group decreased (P = 0.0055) as fertility increased. TH2B was constantly detectable in sperm and expression levels of TH2B decreased in relation to fertility in sperm from the high fertility group (P = 0.018). TH2B biological functions include male gamete generation, chromosome organization, DNA packaging, DNA conformation change, chromatin organization, nucleosome organization, chromatin disassembly, spermatid nucleus elongation, spermatid nucleus differentiation, sperm motility, chromatin organization, chromatin condensation, chromatin silencing, nucleus organization, and chromatin remodeling (P < 0.05). CONCLUSIONS: We elucidated the cellular localization and molecular physiology of TH2B using both computational and cell biology approaches. In addition to advancing the fundamental science of mammalian male gamete, the present findings can be potentially used to evaluate semen quality and predict male fertility in the future. TRIAL REGISTRATION: This study did not involve any live animals. We did not perform any anesthesia, euthanasia, or any kind of animal sacrifice. The cryopreserved semen samples were obtained from Alta Genetics, Inc., Watertown, WI, USA. All samples were preserved in liquid nitrogen. |
format | Online Article Text |
id | pubmed-5539985 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-55399852017-08-03 Testis specific histone 2B is associated with sperm chromatin dynamics and bull fertility-a pilot study Kutchy, Naseer A. Velho, Ana Menezes, Erika S. B. Jacobsen, Marie Thibaudeau, Giselle Wills, Robert W. Moura, Arlindo Kaya, Abdullah Perkins, Andy Memili, Erdogan Reprod Biol Endocrinol Research BACKGROUND: Bull fertility is the degree of sperm’s ability to fertilize and activate the egg and support embryo development, and this is critical for herd reproductive performance. We used the bull as a unique model organism for the study of male fertility because cattle genetics and physiology is similar to those of other mammals including humans. Moreover, reliable fertility data along with well-established in vitro systems are available for bovine. The objective of this original study was to ascertain evolutionary diversification and expression dynamics of Testis Specific Histone 2B (TH2B) in sperm from Holstein bulls with different fertility scores. METHODS: The intensity of TH2B was determined by using flow cytometry in sperm from 13 high and 13 low fertility bulls. Expression levels of TH2B were measured using immunofluorescence and Western blotting in sperm from five high and five low fertility bulls. Sequence identity, evolutionary distance and interactome of TH2B were evaluated by dotmatcher, STRING and Cytoscape. Data were analyzed using linear mixed effects model and regression plots were drawn. RESULTS: The intensity of TH2B as measured by flow cytometry was significantly affected by an interaction between fertility group and fertility score (P = 0.0182). The intensity of TH2B in sperm from the high fertility group decreased (P = 0.0055) as fertility increased. TH2B was constantly detectable in sperm and expression levels of TH2B decreased in relation to fertility in sperm from the high fertility group (P = 0.018). TH2B biological functions include male gamete generation, chromosome organization, DNA packaging, DNA conformation change, chromatin organization, nucleosome organization, chromatin disassembly, spermatid nucleus elongation, spermatid nucleus differentiation, sperm motility, chromatin organization, chromatin condensation, chromatin silencing, nucleus organization, and chromatin remodeling (P < 0.05). CONCLUSIONS: We elucidated the cellular localization and molecular physiology of TH2B using both computational and cell biology approaches. In addition to advancing the fundamental science of mammalian male gamete, the present findings can be potentially used to evaluate semen quality and predict male fertility in the future. TRIAL REGISTRATION: This study did not involve any live animals. We did not perform any anesthesia, euthanasia, or any kind of animal sacrifice. The cryopreserved semen samples were obtained from Alta Genetics, Inc., Watertown, WI, USA. All samples were preserved in liquid nitrogen. BioMed Central 2017-08-01 /pmc/articles/PMC5539985/ /pubmed/28764714 http://dx.doi.org/10.1186/s12958-017-0274-1 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Kutchy, Naseer A. Velho, Ana Menezes, Erika S. B. Jacobsen, Marie Thibaudeau, Giselle Wills, Robert W. Moura, Arlindo Kaya, Abdullah Perkins, Andy Memili, Erdogan Testis specific histone 2B is associated with sperm chromatin dynamics and bull fertility-a pilot study |
title | Testis specific histone 2B is associated with sperm chromatin dynamics and bull fertility-a pilot study |
title_full | Testis specific histone 2B is associated with sperm chromatin dynamics and bull fertility-a pilot study |
title_fullStr | Testis specific histone 2B is associated with sperm chromatin dynamics and bull fertility-a pilot study |
title_full_unstemmed | Testis specific histone 2B is associated with sperm chromatin dynamics and bull fertility-a pilot study |
title_short | Testis specific histone 2B is associated with sperm chromatin dynamics and bull fertility-a pilot study |
title_sort | testis specific histone 2b is associated with sperm chromatin dynamics and bull fertility-a pilot study |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539985/ https://www.ncbi.nlm.nih.gov/pubmed/28764714 http://dx.doi.org/10.1186/s12958-017-0274-1 |
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