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Decreased Serum Sirtuin-1 in COPD
BACKGROUND: The protein deacetylase sirtuin-1 (SIRT1) is an antiaging molecule that is decreased in the lung in patients with COPD. Recently, SIRT1 was reported to be detectable in serum, but serum SIRT1 (s120S) levels have not yet been reported in patients with COPD. METHODS: Serum SIRT1 protein of...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American College of Chest Physicians
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5540026/ https://www.ncbi.nlm.nih.gov/pubmed/28506610 http://dx.doi.org/10.1016/j.chest.2017.05.004 |
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author | Yanagisawa, Satoru Papaioannou, Andriana I. Papaporfyriou, Anastasia Baker, Jonathan R. Vuppusetty, Chaitanya Loukides, Stelios Barnes, Peter J. Ito, Kazuhiro |
author_facet | Yanagisawa, Satoru Papaioannou, Andriana I. Papaporfyriou, Anastasia Baker, Jonathan R. Vuppusetty, Chaitanya Loukides, Stelios Barnes, Peter J. Ito, Kazuhiro |
author_sort | Yanagisawa, Satoru |
collection | PubMed |
description | BACKGROUND: The protein deacetylase sirtuin-1 (SIRT1) is an antiaging molecule that is decreased in the lung in patients with COPD. Recently, SIRT1 was reported to be detectable in serum, but serum SIRT1 (s120S) levels have not yet been reported in patients with COPD. METHODS: Serum SIRT1 protein of all samples was measured by Western blot, and the SIRT1 protein band densities were calculated and compared with clinical parameters. RESULTS: Several molecular sizes of SIRT1, including 120 kDa (actual size) and fragments (102 and 75 kDa) were quantified by Western blot. Among them, only the 120-kDa s120S was significantly decreased in patients with COPD compared with the control subjects without COPD (s120S ratio in healthy subjects = 0.90 ± 0.34 vs those with COPD = 0.68 ± 0.24; P = .014) and was positively correlated with airway obstruction (FEV(1)/FVC, r = 0.31; P = .020); its severity measured by FEV(1) % predicted (r = 0.29; P = .029). s120S also showed a positive correlation with BMI (r = 0.36; P = .0077) and diffusing capacity of the lung per unit volume (the carbon monoxide transfer coefficient: K(CO)%) (r = 0.32; P = .025). It was also significantly decreased with increasing severity of lung emphysema (r = –0.40; P = .027) and with a clinical history of frequent COPD exacerbations (infrequent vs frequent, 0.76 ± 0.20 vs 0.56 ± 0.26; P = .027). SIRT1 was not detected in supernatant of A549 and primary epithelial cells in normal culture conditions. CONCLUSIONS: s120S was decreased in the patients with COPD, potentially as reflected by the reduced SIRT1 within cells as a result of oxidative stress, and might be a potential biomarker for certain disease characteristics of COPD. |
format | Online Article Text |
id | pubmed-5540026 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American College of Chest Physicians |
record_format | MEDLINE/PubMed |
spelling | pubmed-55400262017-08-09 Decreased Serum Sirtuin-1 in COPD Yanagisawa, Satoru Papaioannou, Andriana I. Papaporfyriou, Anastasia Baker, Jonathan R. Vuppusetty, Chaitanya Loukides, Stelios Barnes, Peter J. Ito, Kazuhiro Chest Original Research: COPD BACKGROUND: The protein deacetylase sirtuin-1 (SIRT1) is an antiaging molecule that is decreased in the lung in patients with COPD. Recently, SIRT1 was reported to be detectable in serum, but serum SIRT1 (s120S) levels have not yet been reported in patients with COPD. METHODS: Serum SIRT1 protein of all samples was measured by Western blot, and the SIRT1 protein band densities were calculated and compared with clinical parameters. RESULTS: Several molecular sizes of SIRT1, including 120 kDa (actual size) and fragments (102 and 75 kDa) were quantified by Western blot. Among them, only the 120-kDa s120S was significantly decreased in patients with COPD compared with the control subjects without COPD (s120S ratio in healthy subjects = 0.90 ± 0.34 vs those with COPD = 0.68 ± 0.24; P = .014) and was positively correlated with airway obstruction (FEV(1)/FVC, r = 0.31; P = .020); its severity measured by FEV(1) % predicted (r = 0.29; P = .029). s120S also showed a positive correlation with BMI (r = 0.36; P = .0077) and diffusing capacity of the lung per unit volume (the carbon monoxide transfer coefficient: K(CO)%) (r = 0.32; P = .025). It was also significantly decreased with increasing severity of lung emphysema (r = –0.40; P = .027) and with a clinical history of frequent COPD exacerbations (infrequent vs frequent, 0.76 ± 0.20 vs 0.56 ± 0.26; P = .027). SIRT1 was not detected in supernatant of A549 and primary epithelial cells in normal culture conditions. CONCLUSIONS: s120S was decreased in the patients with COPD, potentially as reflected by the reduced SIRT1 within cells as a result of oxidative stress, and might be a potential biomarker for certain disease characteristics of COPD. American College of Chest Physicians 2017-08 /pmc/articles/PMC5540026/ /pubmed/28506610 http://dx.doi.org/10.1016/j.chest.2017.05.004 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Original Research: COPD Yanagisawa, Satoru Papaioannou, Andriana I. Papaporfyriou, Anastasia Baker, Jonathan R. Vuppusetty, Chaitanya Loukides, Stelios Barnes, Peter J. Ito, Kazuhiro Decreased Serum Sirtuin-1 in COPD |
title | Decreased Serum Sirtuin-1 in COPD |
title_full | Decreased Serum Sirtuin-1 in COPD |
title_fullStr | Decreased Serum Sirtuin-1 in COPD |
title_full_unstemmed | Decreased Serum Sirtuin-1 in COPD |
title_short | Decreased Serum Sirtuin-1 in COPD |
title_sort | decreased serum sirtuin-1 in copd |
topic | Original Research: COPD |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5540026/ https://www.ncbi.nlm.nih.gov/pubmed/28506610 http://dx.doi.org/10.1016/j.chest.2017.05.004 |
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