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Nodal/Activin Pathway is a Conserved Neural Induction Signal in Chordates

Neural induction is the process through which pluripotent cells are committed to a neural fate. This first step of Central Nervous System formation is triggered by the "Spemann organizer" in amphibians and by homologous embryonic regions in other vertebrates. Studies in classical vertebrat...

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Autores principales: Le Petillon, Yann, Luxardi, Guillaume, Scerbo, Pierluigi, Cibois, Marie, Leon, Anthony, Subirana, Lucie, Irimia, Manuel, Kodjabachian, Laurent, Escriva, Hector, Bertrand, Stephanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5540175/
https://www.ncbi.nlm.nih.gov/pubmed/28782045
http://dx.doi.org/10.1038/s41559-017-0226-3
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author Le Petillon, Yann
Luxardi, Guillaume
Scerbo, Pierluigi
Cibois, Marie
Leon, Anthony
Subirana, Lucie
Irimia, Manuel
Kodjabachian, Laurent
Escriva, Hector
Bertrand, Stephanie
author_facet Le Petillon, Yann
Luxardi, Guillaume
Scerbo, Pierluigi
Cibois, Marie
Leon, Anthony
Subirana, Lucie
Irimia, Manuel
Kodjabachian, Laurent
Escriva, Hector
Bertrand, Stephanie
author_sort Le Petillon, Yann
collection PubMed
description Neural induction is the process through which pluripotent cells are committed to a neural fate. This first step of Central Nervous System formation is triggered by the "Spemann organizer" in amphibians and by homologous embryonic regions in other vertebrates. Studies in classical vertebrate models have produced contrasting views about the molecular nature of neural inducers and no unifying scheme could be drawn. Moreover, how this process evolved in the chordate lineage remains an unresolved issue. In this work, by using graft and micromanipulation experiments, we definitively establish that the dorsal blastopore lip of the cephalochordate amphioxus is homologous to the vertebrate organizer and is able to trigger the formation of neural tissues in a host embryo. In addition, we demonstrate that Nodal/Activin is the main signal eliciting neural induction in amphioxus, and that it also functions as a bona fide neural inducer in the classical vertebrate model Xenopus. Altogether, our results allow us to propose that Nodal/Activin was a major player of neural induction in the ancestor of chordates. This study further reveals the diversity of neural inducers deployed during chordate evolution and advocates against a universally conserved molecular explanation for this process.
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spelling pubmed-55401752018-01-03 Nodal/Activin Pathway is a Conserved Neural Induction Signal in Chordates Le Petillon, Yann Luxardi, Guillaume Scerbo, Pierluigi Cibois, Marie Leon, Anthony Subirana, Lucie Irimia, Manuel Kodjabachian, Laurent Escriva, Hector Bertrand, Stephanie Nat Ecol Evol Article Neural induction is the process through which pluripotent cells are committed to a neural fate. This first step of Central Nervous System formation is triggered by the "Spemann organizer" in amphibians and by homologous embryonic regions in other vertebrates. Studies in classical vertebrate models have produced contrasting views about the molecular nature of neural inducers and no unifying scheme could be drawn. Moreover, how this process evolved in the chordate lineage remains an unresolved issue. In this work, by using graft and micromanipulation experiments, we definitively establish that the dorsal blastopore lip of the cephalochordate amphioxus is homologous to the vertebrate organizer and is able to trigger the formation of neural tissues in a host embryo. In addition, we demonstrate that Nodal/Activin is the main signal eliciting neural induction in amphioxus, and that it also functions as a bona fide neural inducer in the classical vertebrate model Xenopus. Altogether, our results allow us to propose that Nodal/Activin was a major player of neural induction in the ancestor of chordates. This study further reveals the diversity of neural inducers deployed during chordate evolution and advocates against a universally conserved molecular explanation for this process. 2017-07-03 2017-08 /pmc/articles/PMC5540175/ /pubmed/28782045 http://dx.doi.org/10.1038/s41559-017-0226-3 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Le Petillon, Yann
Luxardi, Guillaume
Scerbo, Pierluigi
Cibois, Marie
Leon, Anthony
Subirana, Lucie
Irimia, Manuel
Kodjabachian, Laurent
Escriva, Hector
Bertrand, Stephanie
Nodal/Activin Pathway is a Conserved Neural Induction Signal in Chordates
title Nodal/Activin Pathway is a Conserved Neural Induction Signal in Chordates
title_full Nodal/Activin Pathway is a Conserved Neural Induction Signal in Chordates
title_fullStr Nodal/Activin Pathway is a Conserved Neural Induction Signal in Chordates
title_full_unstemmed Nodal/Activin Pathway is a Conserved Neural Induction Signal in Chordates
title_short Nodal/Activin Pathway is a Conserved Neural Induction Signal in Chordates
title_sort nodal/activin pathway is a conserved neural induction signal in chordates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5540175/
https://www.ncbi.nlm.nih.gov/pubmed/28782045
http://dx.doi.org/10.1038/s41559-017-0226-3
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