Cargando…
Lysophosphatidyl Choline Induced Demyelination in Rat Probed by Relaxation along a Fictitious Field in High Rank Rotating Frame
In this work a new MRI modality entitled Relaxation Along a Fictitious Field in the rotating frame of rank 4 (RAFF4) was evaluated in its ability to detect lower myelin content in lysophosphatidyl choline (LPC)-induced demyelinating lesions. The lesions were induced in two areas of the rat brain wit...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5540896/ https://www.ncbi.nlm.nih.gov/pubmed/28824359 http://dx.doi.org/10.3389/fnins.2017.00433 |
_version_ | 1783254702475968512 |
---|---|
author | Lehto, Lauri J. Albors, Aloma A. Sierra, Alejandra Tolppanen, Laura Eberly, Lynn E. Mangia, Silvia Nurmi, Antti Michaeli, Shalom Gröhn, Olli |
author_facet | Lehto, Lauri J. Albors, Aloma A. Sierra, Alejandra Tolppanen, Laura Eberly, Lynn E. Mangia, Silvia Nurmi, Antti Michaeli, Shalom Gröhn, Olli |
author_sort | Lehto, Lauri J. |
collection | PubMed |
description | In this work a new MRI modality entitled Relaxation Along a Fictitious Field in the rotating frame of rank 4 (RAFF4) was evaluated in its ability to detect lower myelin content in lysophosphatidyl choline (LPC)-induced demyelinating lesions. The lesions were induced in two areas of the rat brain with either uniform or complex fiber orientations, i.e., in the corpus callosum (cc) and dorsal tegmental tract (dtg), respectively. RAFF4 showed excellent ability to detect demyelinated lesions and good correlation with myelin content in both brain areas. In comparison, diffusion tensor imaging metrices, fractional anisotropy, mean diffusivity and axonal and radial diffusivity, and magnetization transfer (MT) metrices, longitudinal relaxation during off-resonance irradiation and MT ratio, either failed to detect demyelination in dtg or showed lower correlation with myelin density quantified from gold chloride stained histological sections. Good specifity of RAFF4 to myelin was confirmed by its low correlation with cell density assesed from Nissl stained sections as well as its lack of sensitivity to pH changes in the physiological range as tested in heat denaturated bovine serum albumin phantoms. The excellent ability of RAFF4 to detect myelin content and its insensitivity to fiber orientation distribution, gliosis and pH, together with low specific absorption rate, demonstrates the promise of rotating frame of rank n (RAFFn) as a valuable MRI technique for non-invasive imaging of demyelinating lesions. |
format | Online Article Text |
id | pubmed-5540896 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-55408962017-08-18 Lysophosphatidyl Choline Induced Demyelination in Rat Probed by Relaxation along a Fictitious Field in High Rank Rotating Frame Lehto, Lauri J. Albors, Aloma A. Sierra, Alejandra Tolppanen, Laura Eberly, Lynn E. Mangia, Silvia Nurmi, Antti Michaeli, Shalom Gröhn, Olli Front Neurosci Neuroscience In this work a new MRI modality entitled Relaxation Along a Fictitious Field in the rotating frame of rank 4 (RAFF4) was evaluated in its ability to detect lower myelin content in lysophosphatidyl choline (LPC)-induced demyelinating lesions. The lesions were induced in two areas of the rat brain with either uniform or complex fiber orientations, i.e., in the corpus callosum (cc) and dorsal tegmental tract (dtg), respectively. RAFF4 showed excellent ability to detect demyelinated lesions and good correlation with myelin content in both brain areas. In comparison, diffusion tensor imaging metrices, fractional anisotropy, mean diffusivity and axonal and radial diffusivity, and magnetization transfer (MT) metrices, longitudinal relaxation during off-resonance irradiation and MT ratio, either failed to detect demyelination in dtg or showed lower correlation with myelin density quantified from gold chloride stained histological sections. Good specifity of RAFF4 to myelin was confirmed by its low correlation with cell density assesed from Nissl stained sections as well as its lack of sensitivity to pH changes in the physiological range as tested in heat denaturated bovine serum albumin phantoms. The excellent ability of RAFF4 to detect myelin content and its insensitivity to fiber orientation distribution, gliosis and pH, together with low specific absorption rate, demonstrates the promise of rotating frame of rank n (RAFFn) as a valuable MRI technique for non-invasive imaging of demyelinating lesions. Frontiers Media S.A. 2017-08-03 /pmc/articles/PMC5540896/ /pubmed/28824359 http://dx.doi.org/10.3389/fnins.2017.00433 Text en Copyright © 2017 Lehto, Albors, Sierra, Tolppanen, Eberly, Mangia, Nurmi, Michaeli and Gröhn. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Lehto, Lauri J. Albors, Aloma A. Sierra, Alejandra Tolppanen, Laura Eberly, Lynn E. Mangia, Silvia Nurmi, Antti Michaeli, Shalom Gröhn, Olli Lysophosphatidyl Choline Induced Demyelination in Rat Probed by Relaxation along a Fictitious Field in High Rank Rotating Frame |
title | Lysophosphatidyl Choline Induced Demyelination in Rat Probed by Relaxation along a Fictitious Field in High Rank Rotating Frame |
title_full | Lysophosphatidyl Choline Induced Demyelination in Rat Probed by Relaxation along a Fictitious Field in High Rank Rotating Frame |
title_fullStr | Lysophosphatidyl Choline Induced Demyelination in Rat Probed by Relaxation along a Fictitious Field in High Rank Rotating Frame |
title_full_unstemmed | Lysophosphatidyl Choline Induced Demyelination in Rat Probed by Relaxation along a Fictitious Field in High Rank Rotating Frame |
title_short | Lysophosphatidyl Choline Induced Demyelination in Rat Probed by Relaxation along a Fictitious Field in High Rank Rotating Frame |
title_sort | lysophosphatidyl choline induced demyelination in rat probed by relaxation along a fictitious field in high rank rotating frame |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5540896/ https://www.ncbi.nlm.nih.gov/pubmed/28824359 http://dx.doi.org/10.3389/fnins.2017.00433 |
work_keys_str_mv | AT lehtolaurij lysophosphatidylcholineinduceddemyelinationinratprobedbyrelaxationalongafictitiousfieldinhighrankrotatingframe AT alborsalomaa lysophosphatidylcholineinduceddemyelinationinratprobedbyrelaxationalongafictitiousfieldinhighrankrotatingframe AT sierraalejandra lysophosphatidylcholineinduceddemyelinationinratprobedbyrelaxationalongafictitiousfieldinhighrankrotatingframe AT tolppanenlaura lysophosphatidylcholineinduceddemyelinationinratprobedbyrelaxationalongafictitiousfieldinhighrankrotatingframe AT eberlylynne lysophosphatidylcholineinduceddemyelinationinratprobedbyrelaxationalongafictitiousfieldinhighrankrotatingframe AT mangiasilvia lysophosphatidylcholineinduceddemyelinationinratprobedbyrelaxationalongafictitiousfieldinhighrankrotatingframe AT nurmiantti lysophosphatidylcholineinduceddemyelinationinratprobedbyrelaxationalongafictitiousfieldinhighrankrotatingframe AT michaelishalom lysophosphatidylcholineinduceddemyelinationinratprobedbyrelaxationalongafictitiousfieldinhighrankrotatingframe AT grohnolli lysophosphatidylcholineinduceddemyelinationinratprobedbyrelaxationalongafictitiousfieldinhighrankrotatingframe |