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Application of aptamers for assessment of vaccine efficacy

Assessing antigen concentration of vaccine is essential step in determining the quality of the vaccine prior to vaccination. After vaccination, vaccine-induced antibody titer should also be measured to verify the vaccine efficacy. Since conventional assay used for vaccine concentrations and induced...

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Detalles Bibliográficos
Autores principales: Yoon, Soo Young, Gee, Grace, Hong, Kee-Jong, Seo, Sang-Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Vaccine Society 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5540965/
https://www.ncbi.nlm.nih.gov/pubmed/28775981
http://dx.doi.org/10.7774/cevr.2017.6.2.160
Descripción
Sumario:Assessing antigen concentration of vaccine is essential step in determining the quality of the vaccine prior to vaccination. After vaccination, vaccine-induced antibody titer should also be measured to verify the vaccine efficacy. Since conventional assay used for vaccine concentrations and induced Ab-titers is antibody-based enzyme-linked immunosorbent assay, the assay inevitably brings drawbacks of antibody such as high cost for production, limited stability, and inconsistent quality between lot-to-lots. Aptamer is single-stranded nucleic acid having three-dimensional structure and has features overcoming limitations of antibody. This review will briefly introduce the features of aptamer and potential of aptamer-based system for evaluation of vaccine efficacy.