Cargando…

Collagen-Fibrinogen Lyophilised Scaffolds for Soft Tissue Regeneration

A significant body of research has considered collagen as a scaffold material for soft tissue regeneration. The main structural component of extra-cellular matrix (ECM), collagen’s advantages over synthetic polymers are numerous. However, for applications where higher stiffness and stability are req...

Descripción completa

Detalles Bibliográficos
Autores principales: Shepherd, Jennifer, Bax, Daniel, Best, Serena, Cameron, Ruth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541296/
https://www.ncbi.nlm.nih.gov/pubmed/28772927
http://dx.doi.org/10.3390/ma10060568
_version_ 1783254781770334208
author Shepherd, Jennifer
Bax, Daniel
Best, Serena
Cameron, Ruth
author_facet Shepherd, Jennifer
Bax, Daniel
Best, Serena
Cameron, Ruth
author_sort Shepherd, Jennifer
collection PubMed
description A significant body of research has considered collagen as a scaffold material for soft tissue regeneration. The main structural component of extra-cellular matrix (ECM), collagen’s advantages over synthetic polymers are numerous. However, for applications where higher stiffness and stability are required, significant cross-linking may affect bioactivity. A carbodiimide (EDC) cross-linking route consumes carboxylate groups that are key to collagen’s essential cell recognition motifs (GxOGER). Fibrinogen was considered as a promising additive as it plays a key role in the process of wound repair and contains RGD integrin binding sites which bind to a variety of cells, growth factors and cytokines. Fibrinogen’s binding sites however, also contain the same carboxylate groups as collagen. We have successfully produced highly interconnected, porous collagen-fibrinogen scaffolds using a lyophilisation technique and micro-computed tomography demonstrated minimal influence of either fibrinogen content or cross-linking concentration on the scaffold structure. The specific biological effect of fibrinogen additions into cross-linked collagen are considered by using films as a model for the struts of bulk scaffolds. By considering various additions of fibrinogen to the collagen film with increasing degrees of cross-linking, this study demonstrates a significant biological advantage with fibrinogen addition across the cross-linking concentrations typically applied to collagen-based scaffolds.
format Online
Article
Text
id pubmed-5541296
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-55412962017-08-14 Collagen-Fibrinogen Lyophilised Scaffolds for Soft Tissue Regeneration Shepherd, Jennifer Bax, Daniel Best, Serena Cameron, Ruth Materials (Basel) Article A significant body of research has considered collagen as a scaffold material for soft tissue regeneration. The main structural component of extra-cellular matrix (ECM), collagen’s advantages over synthetic polymers are numerous. However, for applications where higher stiffness and stability are required, significant cross-linking may affect bioactivity. A carbodiimide (EDC) cross-linking route consumes carboxylate groups that are key to collagen’s essential cell recognition motifs (GxOGER). Fibrinogen was considered as a promising additive as it plays a key role in the process of wound repair and contains RGD integrin binding sites which bind to a variety of cells, growth factors and cytokines. Fibrinogen’s binding sites however, also contain the same carboxylate groups as collagen. We have successfully produced highly interconnected, porous collagen-fibrinogen scaffolds using a lyophilisation technique and micro-computed tomography demonstrated minimal influence of either fibrinogen content or cross-linking concentration on the scaffold structure. The specific biological effect of fibrinogen additions into cross-linked collagen are considered by using films as a model for the struts of bulk scaffolds. By considering various additions of fibrinogen to the collagen film with increasing degrees of cross-linking, this study demonstrates a significant biological advantage with fibrinogen addition across the cross-linking concentrations typically applied to collagen-based scaffolds. MDPI 2017-05-23 /pmc/articles/PMC5541296/ /pubmed/28772927 http://dx.doi.org/10.3390/ma10060568 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shepherd, Jennifer
Bax, Daniel
Best, Serena
Cameron, Ruth
Collagen-Fibrinogen Lyophilised Scaffolds for Soft Tissue Regeneration
title Collagen-Fibrinogen Lyophilised Scaffolds for Soft Tissue Regeneration
title_full Collagen-Fibrinogen Lyophilised Scaffolds for Soft Tissue Regeneration
title_fullStr Collagen-Fibrinogen Lyophilised Scaffolds for Soft Tissue Regeneration
title_full_unstemmed Collagen-Fibrinogen Lyophilised Scaffolds for Soft Tissue Regeneration
title_short Collagen-Fibrinogen Lyophilised Scaffolds for Soft Tissue Regeneration
title_sort collagen-fibrinogen lyophilised scaffolds for soft tissue regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541296/
https://www.ncbi.nlm.nih.gov/pubmed/28772927
http://dx.doi.org/10.3390/ma10060568
work_keys_str_mv AT shepherdjennifer collagenfibrinogenlyophilisedscaffoldsforsofttissueregeneration
AT baxdaniel collagenfibrinogenlyophilisedscaffoldsforsofttissueregeneration
AT bestserena collagenfibrinogenlyophilisedscaffoldsforsofttissueregeneration
AT cameronruth collagenfibrinogenlyophilisedscaffoldsforsofttissueregeneration