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Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi)

Biotic and abiotic stresses are the major causes of crop loss in lily worldwide. In this study, we retrieved 12 defense-related expressed sequence tags (ESTs) from the NCBI database and cloned, characterized, and established seven of these genes as stress-induced genes in Lilium formolongi. Using ra...

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Autores principales: Howlader, Jewel, Park, Jong-In, Robin, Arif Hasan Khan, Sumi, Kanij Rukshana, Nou, Ill-Sup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541305/
http://dx.doi.org/10.3390/genes8070172
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author Howlader, Jewel
Park, Jong-In
Robin, Arif Hasan Khan
Sumi, Kanij Rukshana
Nou, Ill-Sup
author_facet Howlader, Jewel
Park, Jong-In
Robin, Arif Hasan Khan
Sumi, Kanij Rukshana
Nou, Ill-Sup
author_sort Howlader, Jewel
collection PubMed
description Biotic and abiotic stresses are the major causes of crop loss in lily worldwide. In this study, we retrieved 12 defense-related expressed sequence tags (ESTs) from the NCBI database and cloned, characterized, and established seven of these genes as stress-induced genes in Lilium formolongi. Using rapid amplification of cDNA ends PCR (RACE-PCR), we successfully cloned seven full-length mRNA sequences from L. formolongi line Sinnapal lily. Based on the presence of highly conserved characteristic domains and phylogenetic analysis using reference protein sequences, we provided new nomenclature for the seven nucleotide and protein sequences and submitted them to GenBank. The real-time quantitative PCR (qPCR) relative expression analysis of these seven genes, including LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfUb, LfCyt-b5, and LfRab, demonstrated that they were differentially expressed in all organs examined, possibly indicating functional redundancy. We also investigated the qPCR relative expression levels under two biotic and four abiotic stress conditions. All seven genes were induced by Botrytis cinerea treatment, and all genes except LfHsp70-3 and LfHsp90 were induced by Botrytis elliptica treatment; these genes might be associated with disease tolerance mechanisms in L. formolongi. In addition, LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfUb, and LfCyt-b5 were induced by heat treatment, LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, and LfCyt-b5 were induced by cold treatment, and LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfCy-b5, and LfRab were induced by drought and salt stress, indicating their likely association with tolerance to these stress conditions. The stress-induced candidate genes identified in this study provide a basis for further functional analysis and the development of stress-resistant L. formolongi cultivars.
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spelling pubmed-55413052017-08-08 Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi) Howlader, Jewel Park, Jong-In Robin, Arif Hasan Khan Sumi, Kanij Rukshana Nou, Ill-Sup Genes (Basel) Article Biotic and abiotic stresses are the major causes of crop loss in lily worldwide. In this study, we retrieved 12 defense-related expressed sequence tags (ESTs) from the NCBI database and cloned, characterized, and established seven of these genes as stress-induced genes in Lilium formolongi. Using rapid amplification of cDNA ends PCR (RACE-PCR), we successfully cloned seven full-length mRNA sequences from L. formolongi line Sinnapal lily. Based on the presence of highly conserved characteristic domains and phylogenetic analysis using reference protein sequences, we provided new nomenclature for the seven nucleotide and protein sequences and submitted them to GenBank. The real-time quantitative PCR (qPCR) relative expression analysis of these seven genes, including LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfUb, LfCyt-b5, and LfRab, demonstrated that they were differentially expressed in all organs examined, possibly indicating functional redundancy. We also investigated the qPCR relative expression levels under two biotic and four abiotic stress conditions. All seven genes were induced by Botrytis cinerea treatment, and all genes except LfHsp70-3 and LfHsp90 were induced by Botrytis elliptica treatment; these genes might be associated with disease tolerance mechanisms in L. formolongi. In addition, LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfUb, and LfCyt-b5 were induced by heat treatment, LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, and LfCyt-b5 were induced by cold treatment, and LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfCy-b5, and LfRab were induced by drought and salt stress, indicating their likely association with tolerance to these stress conditions. The stress-induced candidate genes identified in this study provide a basis for further functional analysis and the development of stress-resistant L. formolongi cultivars. MDPI 2017-06-27 /pmc/articles/PMC5541305/ http://dx.doi.org/10.3390/genes8070172 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Howlader, Jewel
Park, Jong-In
Robin, Arif Hasan Khan
Sumi, Kanij Rukshana
Nou, Ill-Sup
Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi)
title Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi)
title_full Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi)
title_fullStr Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi)
title_full_unstemmed Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi)
title_short Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi)
title_sort identification, characterization and expression profiling of stress-related genes in easter lily (lilium formolongi)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541305/
http://dx.doi.org/10.3390/genes8070172
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