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Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi)
Biotic and abiotic stresses are the major causes of crop loss in lily worldwide. In this study, we retrieved 12 defense-related expressed sequence tags (ESTs) from the NCBI database and cloned, characterized, and established seven of these genes as stress-induced genes in Lilium formolongi. Using ra...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541305/ http://dx.doi.org/10.3390/genes8070172 |
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author | Howlader, Jewel Park, Jong-In Robin, Arif Hasan Khan Sumi, Kanij Rukshana Nou, Ill-Sup |
author_facet | Howlader, Jewel Park, Jong-In Robin, Arif Hasan Khan Sumi, Kanij Rukshana Nou, Ill-Sup |
author_sort | Howlader, Jewel |
collection | PubMed |
description | Biotic and abiotic stresses are the major causes of crop loss in lily worldwide. In this study, we retrieved 12 defense-related expressed sequence tags (ESTs) from the NCBI database and cloned, characterized, and established seven of these genes as stress-induced genes in Lilium formolongi. Using rapid amplification of cDNA ends PCR (RACE-PCR), we successfully cloned seven full-length mRNA sequences from L. formolongi line Sinnapal lily. Based on the presence of highly conserved characteristic domains and phylogenetic analysis using reference protein sequences, we provided new nomenclature for the seven nucleotide and protein sequences and submitted them to GenBank. The real-time quantitative PCR (qPCR) relative expression analysis of these seven genes, including LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfUb, LfCyt-b5, and LfRab, demonstrated that they were differentially expressed in all organs examined, possibly indicating functional redundancy. We also investigated the qPCR relative expression levels under two biotic and four abiotic stress conditions. All seven genes were induced by Botrytis cinerea treatment, and all genes except LfHsp70-3 and LfHsp90 were induced by Botrytis elliptica treatment; these genes might be associated with disease tolerance mechanisms in L. formolongi. In addition, LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfUb, and LfCyt-b5 were induced by heat treatment, LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, and LfCyt-b5 were induced by cold treatment, and LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfCy-b5, and LfRab were induced by drought and salt stress, indicating their likely association with tolerance to these stress conditions. The stress-induced candidate genes identified in this study provide a basis for further functional analysis and the development of stress-resistant L. formolongi cultivars. |
format | Online Article Text |
id | pubmed-5541305 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-55413052017-08-08 Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi) Howlader, Jewel Park, Jong-In Robin, Arif Hasan Khan Sumi, Kanij Rukshana Nou, Ill-Sup Genes (Basel) Article Biotic and abiotic stresses are the major causes of crop loss in lily worldwide. In this study, we retrieved 12 defense-related expressed sequence tags (ESTs) from the NCBI database and cloned, characterized, and established seven of these genes as stress-induced genes in Lilium formolongi. Using rapid amplification of cDNA ends PCR (RACE-PCR), we successfully cloned seven full-length mRNA sequences from L. formolongi line Sinnapal lily. Based on the presence of highly conserved characteristic domains and phylogenetic analysis using reference protein sequences, we provided new nomenclature for the seven nucleotide and protein sequences and submitted them to GenBank. The real-time quantitative PCR (qPCR) relative expression analysis of these seven genes, including LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfUb, LfCyt-b5, and LfRab, demonstrated that they were differentially expressed in all organs examined, possibly indicating functional redundancy. We also investigated the qPCR relative expression levels under two biotic and four abiotic stress conditions. All seven genes were induced by Botrytis cinerea treatment, and all genes except LfHsp70-3 and LfHsp90 were induced by Botrytis elliptica treatment; these genes might be associated with disease tolerance mechanisms in L. formolongi. In addition, LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfUb, and LfCyt-b5 were induced by heat treatment, LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, and LfCyt-b5 were induced by cold treatment, and LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfCy-b5, and LfRab were induced by drought and salt stress, indicating their likely association with tolerance to these stress conditions. The stress-induced candidate genes identified in this study provide a basis for further functional analysis and the development of stress-resistant L. formolongi cultivars. MDPI 2017-06-27 /pmc/articles/PMC5541305/ http://dx.doi.org/10.3390/genes8070172 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Howlader, Jewel Park, Jong-In Robin, Arif Hasan Khan Sumi, Kanij Rukshana Nou, Ill-Sup Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi) |
title | Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi) |
title_full | Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi) |
title_fullStr | Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi) |
title_full_unstemmed | Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi) |
title_short | Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi) |
title_sort | identification, characterization and expression profiling of stress-related genes in easter lily (lilium formolongi) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541305/ http://dx.doi.org/10.3390/genes8070172 |
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