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MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression

Th17 cells and IL-17 participate in airway neutrophil infiltration characteristics in the pathogenesis of severe asthma. Methyl-CpG binding domain protein 2 (MBD2) expression increased in CD4(+) T cells in peripheral blood samples of asthma patients. However, little is known about that epigenetic re...

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Autores principales: Jia, Aijun, Wang, Yueling, Sun, Wenjin, Xiao, Bing, Wei, Yan, Qiu, Lulu, Mu, Lin, Xu, Li, Li, Jianmin, Zhang, Xiufeng, Liu, Da, Peng, Cong, Zhang, Dongshan, Xiang, Xudong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541825/
https://www.ncbi.nlm.nih.gov/pubmed/28808358
http://dx.doi.org/10.1155/2017/6249685
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author Jia, Aijun
Wang, Yueling
Sun, Wenjin
Xiao, Bing
Wei, Yan
Qiu, Lulu
Mu, Lin
Xu, Li
Li, Jianmin
Zhang, Xiufeng
Liu, Da
Peng, Cong
Zhang, Dongshan
Xiang, Xudong
author_facet Jia, Aijun
Wang, Yueling
Sun, Wenjin
Xiao, Bing
Wei, Yan
Qiu, Lulu
Mu, Lin
Xu, Li
Li, Jianmin
Zhang, Xiufeng
Liu, Da
Peng, Cong
Zhang, Dongshan
Xiang, Xudong
author_sort Jia, Aijun
collection PubMed
description Th17 cells and IL-17 participate in airway neutrophil infiltration characteristics in the pathogenesis of severe asthma. Methyl-CpG binding domain protein 2 (MBD2) expression increased in CD4(+) T cells in peripheral blood samples of asthma patients. However, little is known about that epigenetic regulation of MBD2 in both immunological pathogenesis of experimental severe asthma and CD4(+) T cell differentiation. Here, we established a neutrophil-predominant severe asthma model, which was characterized by airway hyperresponsiveness (AHR), BALF neutrophil granulocyte (NEU) increase, higher NEU and IL-17 protein levels, and more Th17 cell differentiation. In the model, MBD2 and IRF4 protein expression increased in the lung and spleen cells. Under overexpression or silencing of the MBD2 and IRF4 gene, the differentiation of Th17 cells and IL-17 secretion showed positive changes. IRF4 protein expression showed a positive change with overexpression or silencing of the MBD2 gene, whereas there was no significant difference in the expression of MBD2 under overexpression or silencing of the IRF4 gene. These data provide novel insights into epigenetic regulation of severe asthma.
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spelling pubmed-55418252017-08-14 MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression Jia, Aijun Wang, Yueling Sun, Wenjin Xiao, Bing Wei, Yan Qiu, Lulu Mu, Lin Xu, Li Li, Jianmin Zhang, Xiufeng Liu, Da Peng, Cong Zhang, Dongshan Xiang, Xudong Mediators Inflamm Research Article Th17 cells and IL-17 participate in airway neutrophil infiltration characteristics in the pathogenesis of severe asthma. Methyl-CpG binding domain protein 2 (MBD2) expression increased in CD4(+) T cells in peripheral blood samples of asthma patients. However, little is known about that epigenetic regulation of MBD2 in both immunological pathogenesis of experimental severe asthma and CD4(+) T cell differentiation. Here, we established a neutrophil-predominant severe asthma model, which was characterized by airway hyperresponsiveness (AHR), BALF neutrophil granulocyte (NEU) increase, higher NEU and IL-17 protein levels, and more Th17 cell differentiation. In the model, MBD2 and IRF4 protein expression increased in the lung and spleen cells. Under overexpression or silencing of the MBD2 and IRF4 gene, the differentiation of Th17 cells and IL-17 secretion showed positive changes. IRF4 protein expression showed a positive change with overexpression or silencing of the MBD2 gene, whereas there was no significant difference in the expression of MBD2 under overexpression or silencing of the IRF4 gene. These data provide novel insights into epigenetic regulation of severe asthma. Hindawi 2017 2017-07-20 /pmc/articles/PMC5541825/ /pubmed/28808358 http://dx.doi.org/10.1155/2017/6249685 Text en Copyright © 2017 Aijun Jia et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jia, Aijun
Wang, Yueling
Sun, Wenjin
Xiao, Bing
Wei, Yan
Qiu, Lulu
Mu, Lin
Xu, Li
Li, Jianmin
Zhang, Xiufeng
Liu, Da
Peng, Cong
Zhang, Dongshan
Xiang, Xudong
MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression
title MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression
title_full MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression
title_fullStr MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression
title_full_unstemmed MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression
title_short MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression
title_sort mbd2 regulates th17 cell differentiation and experimental severe asthma by affecting irf4 expression
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541825/
https://www.ncbi.nlm.nih.gov/pubmed/28808358
http://dx.doi.org/10.1155/2017/6249685
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