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Nanonet force microscopy for measuring forces in single smooth muscle cells of the human aorta

A number of innovative methods exist to measure cell–matrix adhesive forces, but they have yet to accurately describe and quantify the intricate interplay of a cell and its fibrous extracellular matrix (ECM). In cardiovascular pathologies, such as aortic aneurysm, new knowledge on the involvement of...

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Autores principales: Hall, Alexander, Chan, Patrick, Sheets, Kevin, Apperson, Matthew, Delaughter, Christopher, Gleason, Thomas G., Phillippi, Julie A., Nain, Amrinder
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541840/
https://www.ncbi.nlm.nih.gov/pubmed/28450452
http://dx.doi.org/10.1091/mbc.E17-01-0053
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author Hall, Alexander
Chan, Patrick
Sheets, Kevin
Apperson, Matthew
Delaughter, Christopher
Gleason, Thomas G.
Phillippi, Julie A.
Nain, Amrinder
author_facet Hall, Alexander
Chan, Patrick
Sheets, Kevin
Apperson, Matthew
Delaughter, Christopher
Gleason, Thomas G.
Phillippi, Julie A.
Nain, Amrinder
author_sort Hall, Alexander
collection PubMed
description A number of innovative methods exist to measure cell–matrix adhesive forces, but they have yet to accurately describe and quantify the intricate interplay of a cell and its fibrous extracellular matrix (ECM). In cardiovascular pathologies, such as aortic aneurysm, new knowledge on the involvement of cell–matrix forces could lead to elucidation of disease mechanisms. To better understand this dynamics, we measured primary human aortic single smooth muscle cell (SMC) forces using nanonet force microscopy in both inside-out (I-O intrinsic contractility) and outside-in (O-I external perturbation) modes. For SMC populations, we measured the I-O and O-I forces to be 12.9 ± 1.0 and 57.9 ± 2.5 nN, respectively. Exposure of cells to oxidative stress conditions caused a force decrease of 57 and 48% in I-O and O-I modes, respectively, and an increase in migration rate by 2.5-fold. Finally, in O-I mode, we cyclically perturbed cells at constant strain of varying duration to simulate in vivo conditions of the cardiac cycle and found that I-O forces decrease with increasing duration and O-I forces decreased by half at shorter cycle times. Thus our findings highlight the need to study forces exerted and felt by cells simultaneously to comprehensively understand force modulation in cardiovascular disease.
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spelling pubmed-55418402017-09-22 Nanonet force microscopy for measuring forces in single smooth muscle cells of the human aorta Hall, Alexander Chan, Patrick Sheets, Kevin Apperson, Matthew Delaughter, Christopher Gleason, Thomas G. Phillippi, Julie A. Nain, Amrinder Mol Biol Cell Articles A number of innovative methods exist to measure cell–matrix adhesive forces, but they have yet to accurately describe and quantify the intricate interplay of a cell and its fibrous extracellular matrix (ECM). In cardiovascular pathologies, such as aortic aneurysm, new knowledge on the involvement of cell–matrix forces could lead to elucidation of disease mechanisms. To better understand this dynamics, we measured primary human aortic single smooth muscle cell (SMC) forces using nanonet force microscopy in both inside-out (I-O intrinsic contractility) and outside-in (O-I external perturbation) modes. For SMC populations, we measured the I-O and O-I forces to be 12.9 ± 1.0 and 57.9 ± 2.5 nN, respectively. Exposure of cells to oxidative stress conditions caused a force decrease of 57 and 48% in I-O and O-I modes, respectively, and an increase in migration rate by 2.5-fold. Finally, in O-I mode, we cyclically perturbed cells at constant strain of varying duration to simulate in vivo conditions of the cardiac cycle and found that I-O forces decrease with increasing duration and O-I forces decreased by half at shorter cycle times. Thus our findings highlight the need to study forces exerted and felt by cells simultaneously to comprehensively understand force modulation in cardiovascular disease. The American Society for Cell Biology 2017-07-07 /pmc/articles/PMC5541840/ /pubmed/28450452 http://dx.doi.org/10.1091/mbc.E17-01-0053 Text en © 2017 Hall et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Hall, Alexander
Chan, Patrick
Sheets, Kevin
Apperson, Matthew
Delaughter, Christopher
Gleason, Thomas G.
Phillippi, Julie A.
Nain, Amrinder
Nanonet force microscopy for measuring forces in single smooth muscle cells of the human aorta
title Nanonet force microscopy for measuring forces in single smooth muscle cells of the human aorta
title_full Nanonet force microscopy for measuring forces in single smooth muscle cells of the human aorta
title_fullStr Nanonet force microscopy for measuring forces in single smooth muscle cells of the human aorta
title_full_unstemmed Nanonet force microscopy for measuring forces in single smooth muscle cells of the human aorta
title_short Nanonet force microscopy for measuring forces in single smooth muscle cells of the human aorta
title_sort nanonet force microscopy for measuring forces in single smooth muscle cells of the human aorta
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541840/
https://www.ncbi.nlm.nih.gov/pubmed/28450452
http://dx.doi.org/10.1091/mbc.E17-01-0053
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