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CFC1 is a cancer stemness-regulating factor in neuroblastoma
BACKGROUND: Despite the use of aggressive therapy, survival rates among high-risk neuroblastoma (NB) patients remain poor. Cancer stem cells (CSCs) are considered to be critically involved in the recurrence and metastasis of NB and are isolated as NB spheres. METHODS: The gene expression profiling o...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542166/ https://www.ncbi.nlm.nih.gov/pubmed/28620148 http://dx.doi.org/10.18632/oncotarget.18464 |
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author | Chikaraishi, Koji Takenobu, Hisanori Sugino, Ryuichi P. Mukae, Kyosuke Akter, Jesmin Haruta, Masayuki Kurosumi, Masafumi Endo, Takaho A. Koseki, Haruhiko Shimojo, Naoki Ohira, Miki Kamijo, Takehiko |
author_facet | Chikaraishi, Koji Takenobu, Hisanori Sugino, Ryuichi P. Mukae, Kyosuke Akter, Jesmin Haruta, Masayuki Kurosumi, Masafumi Endo, Takaho A. Koseki, Haruhiko Shimojo, Naoki Ohira, Miki Kamijo, Takehiko |
author_sort | Chikaraishi, Koji |
collection | PubMed |
description | BACKGROUND: Despite the use of aggressive therapy, survival rates among high-risk neuroblastoma (NB) patients remain poor. Cancer stem cells (CSCs) are considered to be critically involved in the recurrence and metastasis of NB and are isolated as NB spheres. METHODS: The gene expression profiling of adherent (control) and sphere-forming primary NB cells was conducted using a gene expression microarray. CFC1, which functions in the development of embryos and decides the left-right axis, was strongly expressed in sphere-forming cells only and was related to the unfavorable prognosis of NB patients. The knockdown and overexpression of CFC1 were performed using a lentiviral system in NB cell lines. Sphere formation, cell proliferation, colony formation in soft agar, and xenograft tumor formation were analyzed. RESULTS: The overexpression of CFC1 increased sphere formation, cell growth, and colony formation. These phenotypes, particularly sphere formation, and xenograft tumor formation were significantly suppressed by the knockdown of CFC1. CFC1 inhibited Activin A-induced NB cell differentiation and Smad2 phosphorylation in NB cell lines, indicating its involvement in tumorigenesis related to EGF-CFC co-receptor family molecule pathways. Collectively, these results indicate that CFC1 is a candidate molecule for the development of CSC-targeted therapy for NB. |
format | Online Article Text |
id | pubmed-5542166 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-55421662017-08-07 CFC1 is a cancer stemness-regulating factor in neuroblastoma Chikaraishi, Koji Takenobu, Hisanori Sugino, Ryuichi P. Mukae, Kyosuke Akter, Jesmin Haruta, Masayuki Kurosumi, Masafumi Endo, Takaho A. Koseki, Haruhiko Shimojo, Naoki Ohira, Miki Kamijo, Takehiko Oncotarget Priority Research Paper BACKGROUND: Despite the use of aggressive therapy, survival rates among high-risk neuroblastoma (NB) patients remain poor. Cancer stem cells (CSCs) are considered to be critically involved in the recurrence and metastasis of NB and are isolated as NB spheres. METHODS: The gene expression profiling of adherent (control) and sphere-forming primary NB cells was conducted using a gene expression microarray. CFC1, which functions in the development of embryos and decides the left-right axis, was strongly expressed in sphere-forming cells only and was related to the unfavorable prognosis of NB patients. The knockdown and overexpression of CFC1 were performed using a lentiviral system in NB cell lines. Sphere formation, cell proliferation, colony formation in soft agar, and xenograft tumor formation were analyzed. RESULTS: The overexpression of CFC1 increased sphere formation, cell growth, and colony formation. These phenotypes, particularly sphere formation, and xenograft tumor formation were significantly suppressed by the knockdown of CFC1. CFC1 inhibited Activin A-induced NB cell differentiation and Smad2 phosphorylation in NB cell lines, indicating its involvement in tumorigenesis related to EGF-CFC co-receptor family molecule pathways. Collectively, these results indicate that CFC1 is a candidate molecule for the development of CSC-targeted therapy for NB. Impact Journals LLC 2017-06-13 /pmc/articles/PMC5542166/ /pubmed/28620148 http://dx.doi.org/10.18632/oncotarget.18464 Text en Copyright: © 2017 Chikaraishi et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Priority Research Paper Chikaraishi, Koji Takenobu, Hisanori Sugino, Ryuichi P. Mukae, Kyosuke Akter, Jesmin Haruta, Masayuki Kurosumi, Masafumi Endo, Takaho A. Koseki, Haruhiko Shimojo, Naoki Ohira, Miki Kamijo, Takehiko CFC1 is a cancer stemness-regulating factor in neuroblastoma |
title | CFC1 is a cancer stemness-regulating factor in neuroblastoma |
title_full | CFC1 is a cancer stemness-regulating factor in neuroblastoma |
title_fullStr | CFC1 is a cancer stemness-regulating factor in neuroblastoma |
title_full_unstemmed | CFC1 is a cancer stemness-regulating factor in neuroblastoma |
title_short | CFC1 is a cancer stemness-regulating factor in neuroblastoma |
title_sort | cfc1 is a cancer stemness-regulating factor in neuroblastoma |
topic | Priority Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542166/ https://www.ncbi.nlm.nih.gov/pubmed/28620148 http://dx.doi.org/10.18632/oncotarget.18464 |
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