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Augmented sphingosine 1 phosphate receptor-1 signaling in cardiac fibroblasts induces cardiac hypertrophy and fibrosis through angiotensin II and interleukin-6

Background: Cardiac fibroblasts, together with cardiomyocytes, occupy the majority of cells in the myocardium and are involved in myocardial remodeling. The lysophospholipid mediator sphigosine-1-phosphate (S1P) regulates functions of cardiovascular cells through multiple receptors including S1PR1–S...

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Autores principales: Ohkura, Sei-ichiro, Usui, Soichiro, Takashima, Shin-ichiro, Takuwa, Noriko, Yoshioka, Kazuaki, Okamoto, Yasuo, Inagaki, Yutaka, Sugimoto, Naotoshi, Kitano, Teppei, Takamura, Masayuki, Wada, Takashi, Kaneko, Shuichi, Takuwa, Yoh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542600/
https://www.ncbi.nlm.nih.gov/pubmed/28771545
http://dx.doi.org/10.1371/journal.pone.0182329
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author Ohkura, Sei-ichiro
Usui, Soichiro
Takashima, Shin-ichiro
Takuwa, Noriko
Yoshioka, Kazuaki
Okamoto, Yasuo
Inagaki, Yutaka
Sugimoto, Naotoshi
Kitano, Teppei
Takamura, Masayuki
Wada, Takashi
Kaneko, Shuichi
Takuwa, Yoh
author_facet Ohkura, Sei-ichiro
Usui, Soichiro
Takashima, Shin-ichiro
Takuwa, Noriko
Yoshioka, Kazuaki
Okamoto, Yasuo
Inagaki, Yutaka
Sugimoto, Naotoshi
Kitano, Teppei
Takamura, Masayuki
Wada, Takashi
Kaneko, Shuichi
Takuwa, Yoh
author_sort Ohkura, Sei-ichiro
collection PubMed
description Background: Cardiac fibroblasts, together with cardiomyocytes, occupy the majority of cells in the myocardium and are involved in myocardial remodeling. The lysophospholipid mediator sphigosine-1-phosphate (S1P) regulates functions of cardiovascular cells through multiple receptors including S1PR1–S1PR3. S1PR1 but not other S1P receptors was upregulated in angiotensin II-induced hypertrophic hearts. Therefore, we investigated a role of S1PR1 in fibroblasts for cardiac remodeling by employing transgenic mice that overexpressed S1PR1 under the control of α-smooth muscle actin promoter. In S1PR1-transgenic mouse heart, fibroblasts and/or myofibroblasts were hyperplastic, and those cells as well as vascular smooth muscle cells overexpressed S1PR1. Transgenic mice developed bi-ventricular hypertrophy by 12-week-old and diffuse interstitial fibrosis by 24-week-old without hemodynamic stress. Cardiac remodeling in transgenic mice was associated with greater ERK phosphorylation, upregulation of fetal genes, and systolic dysfunction. Transgenic mouse heart showed increased mRNA expression of angiotensin-converting enzyme and interleukin-6 (IL-6). Isolated fibroblasts from transgenic mice exhibited enhanced generation of angiotensin II, which in turn stimulated IL-6 release. Either an AT1 blocker or angiotensin-converting enzyme inhibitor prevented development of cardiac hypertrophy and fibrosis, systolic dysfunction and increased IL-6 expression in transgenic mice. Finally, administration of anti-IL-6 antibody abolished an increase in tyrosine phosphorylation of STAT3, a major signaling molecule downstream of IL-6, in the transgenic mouse heart and prevented development of cardiac hypertrophy in transgenic mice. These results demonstrate a promoting role of S1PR1 in cardiac fibroblasts for cardiac remodeling, in which angiotensin II—AT1 and IL-6 are involved.
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spelling pubmed-55426002017-08-12 Augmented sphingosine 1 phosphate receptor-1 signaling in cardiac fibroblasts induces cardiac hypertrophy and fibrosis through angiotensin II and interleukin-6 Ohkura, Sei-ichiro Usui, Soichiro Takashima, Shin-ichiro Takuwa, Noriko Yoshioka, Kazuaki Okamoto, Yasuo Inagaki, Yutaka Sugimoto, Naotoshi Kitano, Teppei Takamura, Masayuki Wada, Takashi Kaneko, Shuichi Takuwa, Yoh PLoS One Research Article Background: Cardiac fibroblasts, together with cardiomyocytes, occupy the majority of cells in the myocardium and are involved in myocardial remodeling. The lysophospholipid mediator sphigosine-1-phosphate (S1P) regulates functions of cardiovascular cells through multiple receptors including S1PR1–S1PR3. S1PR1 but not other S1P receptors was upregulated in angiotensin II-induced hypertrophic hearts. Therefore, we investigated a role of S1PR1 in fibroblasts for cardiac remodeling by employing transgenic mice that overexpressed S1PR1 under the control of α-smooth muscle actin promoter. In S1PR1-transgenic mouse heart, fibroblasts and/or myofibroblasts were hyperplastic, and those cells as well as vascular smooth muscle cells overexpressed S1PR1. Transgenic mice developed bi-ventricular hypertrophy by 12-week-old and diffuse interstitial fibrosis by 24-week-old without hemodynamic stress. Cardiac remodeling in transgenic mice was associated with greater ERK phosphorylation, upregulation of fetal genes, and systolic dysfunction. Transgenic mouse heart showed increased mRNA expression of angiotensin-converting enzyme and interleukin-6 (IL-6). Isolated fibroblasts from transgenic mice exhibited enhanced generation of angiotensin II, which in turn stimulated IL-6 release. Either an AT1 blocker or angiotensin-converting enzyme inhibitor prevented development of cardiac hypertrophy and fibrosis, systolic dysfunction and increased IL-6 expression in transgenic mice. Finally, administration of anti-IL-6 antibody abolished an increase in tyrosine phosphorylation of STAT3, a major signaling molecule downstream of IL-6, in the transgenic mouse heart and prevented development of cardiac hypertrophy in transgenic mice. These results demonstrate a promoting role of S1PR1 in cardiac fibroblasts for cardiac remodeling, in which angiotensin II—AT1 and IL-6 are involved. Public Library of Science 2017-08-03 /pmc/articles/PMC5542600/ /pubmed/28771545 http://dx.doi.org/10.1371/journal.pone.0182329 Text en © 2017 Ohkura et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ohkura, Sei-ichiro
Usui, Soichiro
Takashima, Shin-ichiro
Takuwa, Noriko
Yoshioka, Kazuaki
Okamoto, Yasuo
Inagaki, Yutaka
Sugimoto, Naotoshi
Kitano, Teppei
Takamura, Masayuki
Wada, Takashi
Kaneko, Shuichi
Takuwa, Yoh
Augmented sphingosine 1 phosphate receptor-1 signaling in cardiac fibroblasts induces cardiac hypertrophy and fibrosis through angiotensin II and interleukin-6
title Augmented sphingosine 1 phosphate receptor-1 signaling in cardiac fibroblasts induces cardiac hypertrophy and fibrosis through angiotensin II and interleukin-6
title_full Augmented sphingosine 1 phosphate receptor-1 signaling in cardiac fibroblasts induces cardiac hypertrophy and fibrosis through angiotensin II and interleukin-6
title_fullStr Augmented sphingosine 1 phosphate receptor-1 signaling in cardiac fibroblasts induces cardiac hypertrophy and fibrosis through angiotensin II and interleukin-6
title_full_unstemmed Augmented sphingosine 1 phosphate receptor-1 signaling in cardiac fibroblasts induces cardiac hypertrophy and fibrosis through angiotensin II and interleukin-6
title_short Augmented sphingosine 1 phosphate receptor-1 signaling in cardiac fibroblasts induces cardiac hypertrophy and fibrosis through angiotensin II and interleukin-6
title_sort augmented sphingosine 1 phosphate receptor-1 signaling in cardiac fibroblasts induces cardiac hypertrophy and fibrosis through angiotensin ii and interleukin-6
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542600/
https://www.ncbi.nlm.nih.gov/pubmed/28771545
http://dx.doi.org/10.1371/journal.pone.0182329
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