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Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels

Seminal plasma (SP) plays an important role in the motility, viability and maintenance of the fertilizing capacity of mammalian spermatozoa. This study is the first on brown bear (Ursus arctos) SP components, and has two main objectives: 1) to define the SP composition in bear ejaculate and 2) to id...

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Autores principales: Anel-López, L., Ortega-Ferrusola, C., Martínez-Rodríguez, C., Álvarez, M., Borragán, S., Chamorro, C., Peña, F. J., Anel, L., de Paz, P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542667/
https://www.ncbi.nlm.nih.gov/pubmed/28771486
http://dx.doi.org/10.1371/journal.pone.0181776
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author Anel-López, L.
Ortega-Ferrusola, C.
Martínez-Rodríguez, C.
Álvarez, M.
Borragán, S.
Chamorro, C.
Peña, F. J.
Anel, L.
de Paz, P.
author_facet Anel-López, L.
Ortega-Ferrusola, C.
Martínez-Rodríguez, C.
Álvarez, M.
Borragán, S.
Chamorro, C.
Peña, F. J.
Anel, L.
de Paz, P.
author_sort Anel-López, L.
collection PubMed
description Seminal plasma (SP) plays an important role in the motility, viability and maintenance of the fertilizing capacity of mammalian spermatozoa. This study is the first on brown bear (Ursus arctos) SP components, and has two main objectives: 1) to define the SP composition in bear ejaculate and 2) to identify variations in SP composition in relation to high and low levels of testosterone in serum during the breeding season. Forty-eight sperm samples from 30 sexually mature male brown bears (Ursus arctos) were obtained by electroejaculation, and their serum testosterone levels were assessed to sort the animals into 2 groups (high and low testosterone levels, threshold 5 ng/dl). The biochemical and protein compositions of the SP samples were assessed, and sperm motility was analyzed. We found that lactate dehydrogenase was significantly higher in the low-serum-testosterone samples, while concentrations of lipase and Mg(+) values were significantly higher in the high-serum-testosterone samples. In contrast, sperm motility did not significantly differ (P>0.05) between the testosterone level groups (total motility: 74.42.8% in the high-level group vs. 77.1±4.7% in the low-level group). A reference digital model was constructed since there is no information for this wild species. To do this, all gel images were added in a binary multidimensional image and thirty-three spots were identified as the most-repeated spots. An analysis of these proteins was done by qualitative equivalency (isoelectric point and molecular weight) with published data for a bull. SP protein composition was compared between bears with high and low serum testosterone, and three proteins (binder of sperm and two enzymes not identified in the reference bull) showed significant (P<0.05) quantitative differences. We conclude that male bears with high or low serum testosterone levels differs only in some properties of their SP, differences in enzyme LDIP2, energy source LACT2, one protein (similar to BSP1) and Mg ion were identified between these two groups. These data may inform the application of SP to improve bear semen extenders.
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spelling pubmed-55426672017-08-12 Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels Anel-López, L. Ortega-Ferrusola, C. Martínez-Rodríguez, C. Álvarez, M. Borragán, S. Chamorro, C. Peña, F. J. Anel, L. de Paz, P. PLoS One Research Article Seminal plasma (SP) plays an important role in the motility, viability and maintenance of the fertilizing capacity of mammalian spermatozoa. This study is the first on brown bear (Ursus arctos) SP components, and has two main objectives: 1) to define the SP composition in bear ejaculate and 2) to identify variations in SP composition in relation to high and low levels of testosterone in serum during the breeding season. Forty-eight sperm samples from 30 sexually mature male brown bears (Ursus arctos) were obtained by electroejaculation, and their serum testosterone levels were assessed to sort the animals into 2 groups (high and low testosterone levels, threshold 5 ng/dl). The biochemical and protein compositions of the SP samples were assessed, and sperm motility was analyzed. We found that lactate dehydrogenase was significantly higher in the low-serum-testosterone samples, while concentrations of lipase and Mg(+) values were significantly higher in the high-serum-testosterone samples. In contrast, sperm motility did not significantly differ (P>0.05) between the testosterone level groups (total motility: 74.42.8% in the high-level group vs. 77.1±4.7% in the low-level group). A reference digital model was constructed since there is no information for this wild species. To do this, all gel images were added in a binary multidimensional image and thirty-three spots were identified as the most-repeated spots. An analysis of these proteins was done by qualitative equivalency (isoelectric point and molecular weight) with published data for a bull. SP protein composition was compared between bears with high and low serum testosterone, and three proteins (binder of sperm and two enzymes not identified in the reference bull) showed significant (P<0.05) quantitative differences. We conclude that male bears with high or low serum testosterone levels differs only in some properties of their SP, differences in enzyme LDIP2, energy source LACT2, one protein (similar to BSP1) and Mg ion were identified between these two groups. These data may inform the application of SP to improve bear semen extenders. Public Library of Science 2017-08-03 /pmc/articles/PMC5542667/ /pubmed/28771486 http://dx.doi.org/10.1371/journal.pone.0181776 Text en © 2017 Anel-López et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Anel-López, L.
Ortega-Ferrusola, C.
Martínez-Rodríguez, C.
Álvarez, M.
Borragán, S.
Chamorro, C.
Peña, F. J.
Anel, L.
de Paz, P.
Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels
title Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels
title_full Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels
title_fullStr Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels
title_full_unstemmed Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels
title_short Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels
title_sort analysis of seminal plasma from brown bear (ursus arctos) during the breeding season: its relationship with testosterone levels
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542667/
https://www.ncbi.nlm.nih.gov/pubmed/28771486
http://dx.doi.org/10.1371/journal.pone.0181776
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