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Bioremediation of aflatoxin B1-contaminated maize by king oyster mushroom (Pleurotus eryngii)

Aflatoxin B1 (AFB(1)) is the most harmful mycotoxin that occurs as natural contaminant of agricultural commodities, particularly maize. Practical solutions for detoxification of contaminated staples and reduction of agricultural wastes are scarce. We investigated the capability of the white-rot and...

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Autores principales: Branà, Maria Teresa, Cimmarusti, Maria Teresa, Haidukowski, Miriam, Logrieco, Antonio Francesco, Altomare, Claudio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542706/
https://www.ncbi.nlm.nih.gov/pubmed/28771640
http://dx.doi.org/10.1371/journal.pone.0182574
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author Branà, Maria Teresa
Cimmarusti, Maria Teresa
Haidukowski, Miriam
Logrieco, Antonio Francesco
Altomare, Claudio
author_facet Branà, Maria Teresa
Cimmarusti, Maria Teresa
Haidukowski, Miriam
Logrieco, Antonio Francesco
Altomare, Claudio
author_sort Branà, Maria Teresa
collection PubMed
description Aflatoxin B1 (AFB(1)) is the most harmful mycotoxin that occurs as natural contaminant of agricultural commodities, particularly maize. Practical solutions for detoxification of contaminated staples and reduction of agricultural wastes are scarce. We investigated the capability of the white-rot and edible fungus Plerotus eryngii (king oyster mushroom) to degrade AFB(1) both in vitro and in a laboratory-scale mushroom cultivation, using a substrate similar to that routinely used in mushroom farms. In malt extract broth, degradation of AFB(1) (500 ng/mL) by nine isolates of P. eryngii ranged from 81 to 99% after 10 days growth, and reached 100% for all isolates after 30 days. The growth of P. eryngii on solid medium (malt extract-agar, MEA) was significantly reduced at concentrations of AFB(1) 500 ng/mL or higher. However, the addition of 5% wheat straw to the culture medium increased the tolerance of P. eryngii to AFB(1) and no inhibition was observed at a AFB(1) content of 500 ng/mL; degradation of AFB(1) in MEA supplemented with 5% wheat straw and 2.5% (w/v) maize flour was 71–94% after 30 days of growth. Further, AFB(1) degradation by P. eryngii strain ITEM 13681 was tested in a laboratory-scale mushroom cultivation. The mushroom growth medium contained 25% (w/w) of maize spiked with AFB(1) to the final content of 128 μg/kg. Pleurotus eryngii degraded up to 86% of the AFB(1) in 28 days, with no significant reduction of either biological efficiency or mushroom yield. Neither the biomass produced on the mushroom substrate nor the mature basidiocarps contained detectable levels of AFB(1) or its metabolite aflatoxicol, thus ruling out the translocation of these toxins through the fungal thallus. These findings make a contribution towards the development of a novel technology for remediation of AFB(1)- contaminated corn through the exploitation of the degradative capability of P. eryngii and its bioconversion into high nutritional value material intended for feed production.
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spelling pubmed-55427062017-08-12 Bioremediation of aflatoxin B1-contaminated maize by king oyster mushroom (Pleurotus eryngii) Branà, Maria Teresa Cimmarusti, Maria Teresa Haidukowski, Miriam Logrieco, Antonio Francesco Altomare, Claudio PLoS One Research Article Aflatoxin B1 (AFB(1)) is the most harmful mycotoxin that occurs as natural contaminant of agricultural commodities, particularly maize. Practical solutions for detoxification of contaminated staples and reduction of agricultural wastes are scarce. We investigated the capability of the white-rot and edible fungus Plerotus eryngii (king oyster mushroom) to degrade AFB(1) both in vitro and in a laboratory-scale mushroom cultivation, using a substrate similar to that routinely used in mushroom farms. In malt extract broth, degradation of AFB(1) (500 ng/mL) by nine isolates of P. eryngii ranged from 81 to 99% after 10 days growth, and reached 100% for all isolates after 30 days. The growth of P. eryngii on solid medium (malt extract-agar, MEA) was significantly reduced at concentrations of AFB(1) 500 ng/mL or higher. However, the addition of 5% wheat straw to the culture medium increased the tolerance of P. eryngii to AFB(1) and no inhibition was observed at a AFB(1) content of 500 ng/mL; degradation of AFB(1) in MEA supplemented with 5% wheat straw and 2.5% (w/v) maize flour was 71–94% after 30 days of growth. Further, AFB(1) degradation by P. eryngii strain ITEM 13681 was tested in a laboratory-scale mushroom cultivation. The mushroom growth medium contained 25% (w/w) of maize spiked with AFB(1) to the final content of 128 μg/kg. Pleurotus eryngii degraded up to 86% of the AFB(1) in 28 days, with no significant reduction of either biological efficiency or mushroom yield. Neither the biomass produced on the mushroom substrate nor the mature basidiocarps contained detectable levels of AFB(1) or its metabolite aflatoxicol, thus ruling out the translocation of these toxins through the fungal thallus. These findings make a contribution towards the development of a novel technology for remediation of AFB(1)- contaminated corn through the exploitation of the degradative capability of P. eryngii and its bioconversion into high nutritional value material intended for feed production. Public Library of Science 2017-08-03 /pmc/articles/PMC5542706/ /pubmed/28771640 http://dx.doi.org/10.1371/journal.pone.0182574 Text en © 2017 Branà et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Branà, Maria Teresa
Cimmarusti, Maria Teresa
Haidukowski, Miriam
Logrieco, Antonio Francesco
Altomare, Claudio
Bioremediation of aflatoxin B1-contaminated maize by king oyster mushroom (Pleurotus eryngii)
title Bioremediation of aflatoxin B1-contaminated maize by king oyster mushroom (Pleurotus eryngii)
title_full Bioremediation of aflatoxin B1-contaminated maize by king oyster mushroom (Pleurotus eryngii)
title_fullStr Bioremediation of aflatoxin B1-contaminated maize by king oyster mushroom (Pleurotus eryngii)
title_full_unstemmed Bioremediation of aflatoxin B1-contaminated maize by king oyster mushroom (Pleurotus eryngii)
title_short Bioremediation of aflatoxin B1-contaminated maize by king oyster mushroom (Pleurotus eryngii)
title_sort bioremediation of aflatoxin b1-contaminated maize by king oyster mushroom (pleurotus eryngii)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542706/
https://www.ncbi.nlm.nih.gov/pubmed/28771640
http://dx.doi.org/10.1371/journal.pone.0182574
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