Visualizing adenosine to inosine RNA editing in single mammalian cells

Conversion of adenosine to inosine is a frequent type of RNA editing, but important details about its biology remain unknown due to a lack of imaging tools. We developed inoFISH to directly visualize and quantify adenosine-to-inosine edited transcripts in situ. We found that editing of GRIA2, EIF2AK...

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Detalles Bibliográficos
Autores principales: Mellis, Ian A., Gupte, Rohit, Raj, Arjun, Rouhanifard, Sara H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542810/
https://www.ncbi.nlm.nih.gov/pubmed/28604724
http://dx.doi.org/10.1038/nmeth.4332
Descripción
Sumario:Conversion of adenosine to inosine is a frequent type of RNA editing, but important details about its biology remain unknown due to a lack of imaging tools. We developed inoFISH to directly visualize and quantify adenosine-to-inosine edited transcripts in situ. We found that editing of GRIA2, EIF2AK2, and NUP43 is uncorrelated with nuclear localization and paraspeckle association. Further, NUP43 exhibits constant editing levels between single cells while GRIA2 levels vary.

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