Visualizing adenosine to inosine RNA editing in single mammalian cells

Conversion of adenosine to inosine is a frequent type of RNA editing, but important details about its biology remain unknown due to a lack of imaging tools. We developed inoFISH to directly visualize and quantify adenosine-to-inosine edited transcripts in situ. We found that editing of GRIA2, EIF2AK...

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Detalles Bibliográficos
Autores principales: Mellis, Ian A., Gupte, Rohit, Raj, Arjun, Rouhanifard, Sara H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542810/
https://www.ncbi.nlm.nih.gov/pubmed/28604724
http://dx.doi.org/10.1038/nmeth.4332
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author Mellis, Ian A.
Gupte, Rohit
Raj, Arjun
Rouhanifard, Sara H.
author_facet Mellis, Ian A.
Gupte, Rohit
Raj, Arjun
Rouhanifard, Sara H.
author_sort Mellis, Ian A.
collection PubMed
description Conversion of adenosine to inosine is a frequent type of RNA editing, but important details about its biology remain unknown due to a lack of imaging tools. We developed inoFISH to directly visualize and quantify adenosine-to-inosine edited transcripts in situ. We found that editing of GRIA2, EIF2AK2, and NUP43 is uncorrelated with nuclear localization and paraspeckle association. Further, NUP43 exhibits constant editing levels between single cells while GRIA2 levels vary.
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spelling pubmed-55428102017-12-12 Visualizing adenosine to inosine RNA editing in single mammalian cells Mellis, Ian A. Gupte, Rohit Raj, Arjun Rouhanifard, Sara H. Nat Methods Article Conversion of adenosine to inosine is a frequent type of RNA editing, but important details about its biology remain unknown due to a lack of imaging tools. We developed inoFISH to directly visualize and quantify adenosine-to-inosine edited transcripts in situ. We found that editing of GRIA2, EIF2AK2, and NUP43 is uncorrelated with nuclear localization and paraspeckle association. Further, NUP43 exhibits constant editing levels between single cells while GRIA2 levels vary. 2017-06-12 2017-08 /pmc/articles/PMC5542810/ /pubmed/28604724 http://dx.doi.org/10.1038/nmeth.4332 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Mellis, Ian A.
Gupte, Rohit
Raj, Arjun
Rouhanifard, Sara H.
Visualizing adenosine to inosine RNA editing in single mammalian cells
title Visualizing adenosine to inosine RNA editing in single mammalian cells
title_full Visualizing adenosine to inosine RNA editing in single mammalian cells
title_fullStr Visualizing adenosine to inosine RNA editing in single mammalian cells
title_full_unstemmed Visualizing adenosine to inosine RNA editing in single mammalian cells
title_short Visualizing adenosine to inosine RNA editing in single mammalian cells
title_sort visualizing adenosine to inosine rna editing in single mammalian cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542810/
https://www.ncbi.nlm.nih.gov/pubmed/28604724
http://dx.doi.org/10.1038/nmeth.4332
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