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Effects of surface functionalization of hydrophilic NaYF(4) nanocrystals doped with Eu(3+) on glutamate and GABA transport in brain synaptosomes

Specific rare earth doped nanocrystals (NCs), a recent class of nanoparticles with fluorescent features, have great bioanalytical potential. Neuroactive properties of NaYF(4) nanocrystals doped with Eu(3+) were assessed based on the analysis of their effects on glutamate- and γ-aminobutyric acid (GA...

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Autores principales: Sojka, Bartlomiej, Kociołek, Daria, Banski, Mateusz, Borisova, Tatiana, Pozdnyakova, Natalia, Pastukhov, Artem, Borysov, Arsenii, Dudarenko, Marina, Podhorodecki, Artur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5543196/
https://www.ncbi.nlm.nih.gov/pubmed/28824289
http://dx.doi.org/10.1007/s11051-017-3958-8
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author Sojka, Bartlomiej
Kociołek, Daria
Banski, Mateusz
Borisova, Tatiana
Pozdnyakova, Natalia
Pastukhov, Artem
Borysov, Arsenii
Dudarenko, Marina
Podhorodecki, Artur
author_facet Sojka, Bartlomiej
Kociołek, Daria
Banski, Mateusz
Borisova, Tatiana
Pozdnyakova, Natalia
Pastukhov, Artem
Borysov, Arsenii
Dudarenko, Marina
Podhorodecki, Artur
author_sort Sojka, Bartlomiej
collection PubMed
description Specific rare earth doped nanocrystals (NCs), a recent class of nanoparticles with fluorescent features, have great bioanalytical potential. Neuroactive properties of NaYF(4) nanocrystals doped with Eu(3+) were assessed based on the analysis of their effects on glutamate- and γ-aminobutyric acid (GABA) transport process in nerve terminals isolated from rat brain (synaptosomes). Two types of hydrophilic NCs were examined in this work: (i) coated by polyethylene glycol (PEG) and (ii) with OH groups at the surface. It was found that NaYF(4):Eu(3+)-PEG and NaYF(4):Eu(3+)-OH within the concentration range of 0.5–3.5 and 0.5–1.5 mg/ml, respectively, did not influence Na(+)-dependent transporter-dependent l-[(14)C]glutamate and [(3)H]GABA uptake and the ambient level of the neurotransmitters in the synaptosomes. An increase in NaYF(4):Eu(3+)-PEG and NaYF(4):Eu(3+)-OH concentrations up to 7.5 and 3.5 mg/ml, respectively, led to the (1) attenuation of the initial velocity of uptake of l-[(14)C]glutamate and [(3)H]GABA and (2) elevation of ambient neurotransmitters in the suspension of nerve terminals. In the mentioned concentrations, nanocrystals did not influence acidification of synaptic vesicles that was shown with pH-sensitive fluorescent dye acridine orange, however, decreased the potential of the plasma membrane of synaptosomes. In comparison with other nanoparticles studied with similar methodological approach, NCs start to exhibit their effects on neurotransmitter transport at concentrations several times higher than those shown for carbon dots, detonation nanodiamonds and an iron storage protein ferritin, whose activity can be registered at 0.08, 0.5 and 0.08 mg/ml, respectively. Therefore, NCs can be considered lesser neurotoxic as compared to above nanoparticles.
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spelling pubmed-55431962017-08-17 Effects of surface functionalization of hydrophilic NaYF(4) nanocrystals doped with Eu(3+) on glutamate and GABA transport in brain synaptosomes Sojka, Bartlomiej Kociołek, Daria Banski, Mateusz Borisova, Tatiana Pozdnyakova, Natalia Pastukhov, Artem Borysov, Arsenii Dudarenko, Marina Podhorodecki, Artur J Nanopart Res Research Paper Specific rare earth doped nanocrystals (NCs), a recent class of nanoparticles with fluorescent features, have great bioanalytical potential. Neuroactive properties of NaYF(4) nanocrystals doped with Eu(3+) were assessed based on the analysis of their effects on glutamate- and γ-aminobutyric acid (GABA) transport process in nerve terminals isolated from rat brain (synaptosomes). Two types of hydrophilic NCs were examined in this work: (i) coated by polyethylene glycol (PEG) and (ii) with OH groups at the surface. It was found that NaYF(4):Eu(3+)-PEG and NaYF(4):Eu(3+)-OH within the concentration range of 0.5–3.5 and 0.5–1.5 mg/ml, respectively, did not influence Na(+)-dependent transporter-dependent l-[(14)C]glutamate and [(3)H]GABA uptake and the ambient level of the neurotransmitters in the synaptosomes. An increase in NaYF(4):Eu(3+)-PEG and NaYF(4):Eu(3+)-OH concentrations up to 7.5 and 3.5 mg/ml, respectively, led to the (1) attenuation of the initial velocity of uptake of l-[(14)C]glutamate and [(3)H]GABA and (2) elevation of ambient neurotransmitters in the suspension of nerve terminals. In the mentioned concentrations, nanocrystals did not influence acidification of synaptic vesicles that was shown with pH-sensitive fluorescent dye acridine orange, however, decreased the potential of the plasma membrane of synaptosomes. In comparison with other nanoparticles studied with similar methodological approach, NCs start to exhibit their effects on neurotransmitter transport at concentrations several times higher than those shown for carbon dots, detonation nanodiamonds and an iron storage protein ferritin, whose activity can be registered at 0.08, 0.5 and 0.08 mg/ml, respectively. Therefore, NCs can be considered lesser neurotoxic as compared to above nanoparticles. Springer Netherlands 2017-08-04 2017 /pmc/articles/PMC5543196/ /pubmed/28824289 http://dx.doi.org/10.1007/s11051-017-3958-8 Text en © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research Paper
Sojka, Bartlomiej
Kociołek, Daria
Banski, Mateusz
Borisova, Tatiana
Pozdnyakova, Natalia
Pastukhov, Artem
Borysov, Arsenii
Dudarenko, Marina
Podhorodecki, Artur
Effects of surface functionalization of hydrophilic NaYF(4) nanocrystals doped with Eu(3+) on glutamate and GABA transport in brain synaptosomes
title Effects of surface functionalization of hydrophilic NaYF(4) nanocrystals doped with Eu(3+) on glutamate and GABA transport in brain synaptosomes
title_full Effects of surface functionalization of hydrophilic NaYF(4) nanocrystals doped with Eu(3+) on glutamate and GABA transport in brain synaptosomes
title_fullStr Effects of surface functionalization of hydrophilic NaYF(4) nanocrystals doped with Eu(3+) on glutamate and GABA transport in brain synaptosomes
title_full_unstemmed Effects of surface functionalization of hydrophilic NaYF(4) nanocrystals doped with Eu(3+) on glutamate and GABA transport in brain synaptosomes
title_short Effects of surface functionalization of hydrophilic NaYF(4) nanocrystals doped with Eu(3+) on glutamate and GABA transport in brain synaptosomes
title_sort effects of surface functionalization of hydrophilic nayf(4) nanocrystals doped with eu(3+) on glutamate and gaba transport in brain synaptosomes
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5543196/
https://www.ncbi.nlm.nih.gov/pubmed/28824289
http://dx.doi.org/10.1007/s11051-017-3958-8
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